This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bussière, F.
Right arrow Articles by Perreault, J.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bussière, F.
Right arrow Articles by Perreault, J.-P.

 Previous Article  |  Next Article 

Journal of Virology, August 1999, p. 6353-6360, Vol. 73, No. 8
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Subcellular Localization and Rolling Circle Replication of Peach Latent Mosaic Viroid: Hallmarks of Group A Viroids

F. Bussière,1 J. Lehoux,1 D. A. Thompson,2 L. J. Skrzeczkowski,3 and J.-P. Perreault1,*

Département de biochimie, Faculté de médecine, Université de Sherbrooke, Sherbrooke, Québec J1H 5N4,1 and Centre for Plant Health, CFIA, Sidney, British Columbia V8L 1H3,2 Canada, and Department of Plant Pathology, Washington State University, Prosser, Washington 993503

Received 15 December 1998/Accepted 23 April 1999

We characterized the peach latent mosaic viroid (PLMVd) replication intermediates that accumulate in infected peach leaves and determined the tissue and subcellular localization of the RNA species. Using in situ hybridization, we showed that PLMVd strands of both plus and minus polarities concentrate in the cells forming the palisade parenchyma. At the cellular level, PLMVd was found to accumulate predominantly in chloroplasts. Northern blot analyses demonstrated that PLMVd replicates via a symmetric mode involving the accumulation of both circular and linear monomeric strands of both polarities. No multimeric conformer was detected, indicating that both strands self-cleave efficiently via their hammerhead sequences. Dot blot hybridizations revealed that PLMVd strands of both polarities accumulate equally but that the relative concentrations vary by more than 50-fold between peach cultivars. Taken together these results establish two hallmarks for the classification of viroids. Group A viroids (e.g., PLMVd), which possess hammerhead structures, replicate in the chloroplasts via the symmetric mode. By contrast, group B viroids, which share a conserved central region, replicate in the nucleus via an asymmetric mechanism. This is an important difference between self-cleaving and non-self-cleaving viroids, and the implications for the evolutionary origin and replication are discussed.

* Corresponding author. Mailing address: Département de biochimie, Université de Sherbrooke, 3001 12e Ave., Sherbrooke, Québec J1H 5N4, Canada. Phone: (819) 564-5310. Fax: (819) 564-5340. E-mail: jperre01{at}courrier.usherb.ca.

Journal of Virology, August 1999, p. 6353-6360, Vol. 73, No. 8
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Martinez, F., Marques, J., Salvador, M. L., Daros, J.-A. (2009). Mutational analysis of eggplant latent viroid RNA processing in Chlamydomonas reinhardtii chloroplast. J. Gen. Virol. 90: 3057-3065 [Abstract] [Full Text]  
  • Dube, A., Bisaillon, M., Perreault, J.-P. (2009). Identification of Proteins from Prunus persica That Interact with Peach Latent Mosaic Viroid. J. Virol. 83: 12057-12067 [Abstract] [Full Text]  
  • Rodio, M.-E., Delgado, S., De Stradis, A., Gomez, M.-D., Flores, R., Di Serio, F. (2007). A Viroid RNA with a Specific Structural Motif Inhibits Chloroplast Development. Plant Cell 19: 3610-3626 [Abstract] [Full Text]  
  • Hernandez, C., Di Serio, F., Ambros, S., Daros, J.-A., Flores, R. (2006). An Element of the Tertiary Structure of Peach Latent Mosaic Viroid RNA Revealed by UV Irradiation.. J. Virol. 80: 9336-9340 [Abstract] [Full Text]  
  • Delgado, S., Martinez de Alba, A. E., Hernandez, C., Flores, R. (2005). A Short Double-Stranded RNA Motif of Peach Latent Mosaic Viroid Contains the Initiation and the Self-Cleavage Sites of Both Polarity Strands. J. Virol. 79: 12934-12943 [Abstract] [Full Text]  
  • Landry, P., Perreault, J.-P. (2005). Identification of a Peach Latent Mosaic Viroid Hairpin Able To Act as a Dicer-Like Substrate. J. Virol. 79: 6540-6543 [Abstract] [Full Text]  
  • Daros, J.-A., Flores, R. (2004). Arabidopsis thaliana has the enzymatic machinery for replicating representative viroid species of the family Pospiviroidae. Proc. Natl. Acad. Sci. USA 101: 6792-6797 [Abstract] [Full Text]  
  • Schrader, O., Baumstark, T., Riesner, D. (2003). A Mini-RNA containing the tetraloop, wobble-pair and loop E motifs of the central conserved region of potato spindle tuber viroid is processed into a minicircle. Nucleic Acids Res 31: 988-998 [Abstract] [Full Text]  
  • Côté, F., Lévesque, D., Perreault, J.-P. (2001). Natural 2',5'-Phosphodiester Bonds Found at the Ligation Sites of Peach Latent Mosaic Viroid. J. Virol. 75: 19-25 [Abstract] [Full Text]  
  • Bussière, F., Ouellet, J., Côté, F., Lévesque, D., Perreault, J. P. (2000). Mapping in Solution Shows the Peach Latent Mosaic Viroid To Possess a New Pseudoknot in a Complex, Branched Secondary Structure. J. Virol. 74: 2647-2654 [Abstract] [Full Text]  
  • De la Pena, M., Flores, R. (2001). An Extra Nucleotide in the Consensus Catalytic Core of a Viroid Hammerhead Ribozyme. IMPLICATIONS FOR THE DESIGN OF MORE EFFICIENT RIBOZYMES. J. Biol. Chem. 276: 34586-34593 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»