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Journal of Virology, July 1999, p. 6182-6187, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Subgenus-Specific C-Terminal Region of Protein IX Is Located on the Surface of the Adenovirus Capsid

Abebe Akalu,1,dagger Herbert Liebermann,1 Ulrike Bauer,1 Harald Granzow,2 and Werner Seidel1,*

Institute of Medical Microbiology, Ernst-Moritz-Arndt-University, D-17487 Greifswald,1 and Institute of Applied Virology, Friedrich-Loeffler-Institutes, Federal Research Center for Virus Diseases of Animals, D-17498 Insel Riems,2 Germany

Received 28 December 1998/Accepted 30 March 1999

We have investigated the antigenicity of the C- and N-terminal halves of pIX of human adenovirus types 2 and 3 (Ad2 and Ad3) as well as their orientations in virions. We found that only the C-terminal halves of Ad2 pIX and Ad3 pIX reacted in a subgenus-specific manner by enzyme-linked immunosorbent assay and immunoblot analysis. Based on immunoelectron microscopy experiments, pIX in viral capsids appears to be positioned such that the C-terminal part of pIX constitutes the surface domain whereas the N terminus of the protein makes up the internal domain in icosahedral Ad capsids.

* Corresponding author. Mailing address: Abteilung Virologie, Institut für Medizinische Mikrobiologie, Martin-Luther-Str. 6, D-17487 Greifswald, Germany. Phone: 49-(0) 3834 865567. Fax: 49-(0) 3834 865568. E-mail: seidel{at}rz.uni-greifswald.de.

dagger Present address: Institute for Genetic Medicine and Department of Biochemistry and Molecular Biology, USC School of Medicine, Los Angeles, CA 90033.

Journal of Virology, July 1999, p. 6182-6187, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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