Journal of Virology, July 1999, p. 6141-6146, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Department of Microbiology,
Received 20 November 1998/Accepted 22 March 1999
One of the limitations of adenovirus vectors is the lack of
machinery necessary for their integration into host chromosomes, resulting in short-term gene expression in dividing cells. We analyzed
frequencies of integration and persistence of gene expression from
integrated adenovirus vectors. Both E1-substituted and helper-dependent adenovirus vectors achieved similar integration efficiencies of ~10
3 to 10
5 per cell, with the
helper-dependent vector showing slightly higher efficiencies. In stable
cell pools, gene expression of the integrated vector persisted for at
least 50 cell divisions without selection. However, some stable cell
clones showed changes in gene expression, which were accompanied by
structural changes in the integrated vector DNA.
*
Corresponding author. Mailing address: Department of
Microbiology, Immunology and Molecular Genetics, UCLA School of
Medicine, Box 951747, Los Angeles, CA 90095-1747. Phone: (310)
267-2031. Fax: (310) 206-3865. E-mail: mitani{at}ucla.edu.
This article has been cited by other articles:
| J. Bacteriol. | Mol. Cell. Biol. | Microbiol. Mol. Biol. Rev. |
|---|
| Clin. Vaccine Immunol. | ALL ASM JOURNALS |
|---|