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Journal of Virology, July 1999, p. 6114-6116, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Retroviral Vectors Pseudotyped with Lymphocytic Choriomeningitis Virus

Hrvoje Miletic,1 Michael Bruns,1 Konstantinos Tsiakas,1 Birgit Vogt,1 Roya Rezai,1 Christopher Baum,1 Klaus Kühlke,2 François-Loïc Cosset,3 Wolfram Ostertag,1 Heinz Lother,1 and Dorothee von Laer1,*

Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, 20251 Hamburg,1 and Eufets, 55743 Idar-Oberstein,2 Germany, and Centre de Génétique Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, UMR5534, UCB Lyon-1, 69622 Villeurbanne Cedex, France3

Received 9 December 1998/Accepted 16 March 1999

Pseudotyping can improve retroviral vector stability and transduction efficiency. Here, we describe a novel pseudotype of murine leukemia virus packaged with lymphocytic choriomeningitis virus (LCMV). This pseudotype was stable during ultracentrifugation and infected several cell lines from different species. Moreover, LCMV glycoproteins were not cell toxic.

* Corresponding author. Mailing address: Heinrich-Pette-Institut, Martinistr. 52, D-20251 Hamburg, Germany. Phone: 49-40-48051274. Fax: 49-40-48051187. E-mail: laer{at}hpi.uni-hamburg.de.

Journal of Virology, July 1999, p. 6114-6116, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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