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Journal of Virology, July 1999, p. 6111-6113, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Interaction of Eukaryotic Initiation Factor eIF4B with the Internal Ribosome Entry Site of Foot-and-Mouth Disease Virus Is Independent of the Polypyrimidine Tract-Binding Protein

René C. Rust,dagger Kerstin Ochs, Karsten Meyer,Dagger Ewald Beck, and Michael Niepmann*

Institute of Biochemistry, D-35392 Giessen, Germany

Received 4 November 1998/Accepted 6 April 1999

Eukaryotic translation initiation factor 4B (eIF4B) binds directly to the internal ribosome entry site (IRES) of foot-and-mouth disease virus (FMDV). Mutations in all three subdomains of the IRES stem-loop 4 reduce binding of eIF4B and translation efficiency in parallel, indicating that eIF4B is functionally involved in FMDV translation initiation. In reticulocyte lysate devoid of polypyrimidine tract-binding protein (PTB), eIF4B still bound well to the wild-type IRES, even after removal of the major PTB-binding site. In conclusion, the interaction of eIF4B with the FMDV IRES is essential for IRES function but independent of PTB.

* Corresponding author. Mailing address: Institute of Biochemistry, Friedrichstrasse 24, D-35392 Giessen, Germany. Phone: 49-641-99-47421. Fax: 49-641-99-47429. E-mail: michael.niepmann{at}biochemie.med.uni-giessen.de.

dagger Present address: Institute of Medical Microbiology, University of Basel, CH-4003 Basel, Switzerland.

Dagger Present address: NOVARTIS Pharma AG, Basel St. Johann, Switzerland.

Journal of Virology, July 1999, p. 6111-6113, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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