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Journal of Virology, July 1999, p. 6104-6110, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Dengue Virus Type 1 Nonstructural Glycoprotein NS1 Is Secreted from Mammalian Cells as a Soluble Hexamer in a Glycosylation-Dependent Fashion

Marie Flamand,1,* Françoise Megret,1 Magali Mathieu,2,dagger Jean Lepault,3 Félix A. Rey,2,dagger and Vincent Deubel1

Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, 75724 Paris Cedex 15,1 and Laboratoire d'Enzymologie et de Biochimie Structurales, CNRS UPR 9063,2 and Centre de Génétique Moléculaire, CNRS UPR 9061,3 91198 Gif-sur-Yvette Cedex, France

Received 21 December 1998/Accepted 1 April 1999

Nonstructural glycoprotein NS1, specified by dengue virus type 1 (Den-1), is secreted from infected green monkey kidney (Vero) cells in a major soluble form characterized by biochemical and biophysical means as a unique hexameric species. This noncovalently bound oligomer is formed by three dimeric subunits and has a molecular mass of 310 kDa and a Stokes radius of 64.4 Å. During protein export, one of the two oligosaccharides of NS1 is processed into an endo-beta -N-acetylglucosaminidase F-resistant complex-type sugar while the other remains of the polymannose type, protected in the dimeric subunit from the action of maturation enzymes. Complete processing of the complex-type sugar appears to be required for efficient release of soluble NS1 into the culture fluid of infected cells, as suggested by the repressive effects of the N-glycan processing inhibitors swainsonine and deoxymannojyrimicin. These results, together with observations related to the absence of secretion of NS1 from Den-infected insect cells, suggest that maturation and secretion of hexameric NS1 depend on the glycosylation status of the host cell.

* Corresponding author. Mailing address: Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France. Phone: (33) 1 40 61 35 63. Fax: (33) 1 40 61 37 74. E-mail: mflamand{at}pasteur.fr.

dagger Present address: Laboratoire de Génétique des Virus, CNRS UPR 9053, 91198 Gif-sur-Yvette Cedex, France.

Journal of Virology, July 1999, p. 6104-6110, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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