This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Plotnicky-Gilquin, H.
Right arrow Articles by Power, U. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Plotnicky-Gilquin, H.
Right arrow Articles by Power, U. F.

 Previous Article  |  Next Article 

Journal of Virology, July 1999, p. 5637-5645, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Multiple Protective Epitopes (Protectopes) in the Central Conserved Domain of a Prototype Human Respiratory Syncytial Virus G Protein

Hélène Plotnicky-Gilquin, Liliane Goetsch, Thierry Huss, Thierry Champion, Alain Beck, Jean-François Haeuw, Thien Ngoc Nguyen, Jean-Yves Bonnefoy, Nathalie Corvaïa, and Ultan F. Power*

Centre d'Immunologie Pierre Fabre, 74164 Saint-Julien-en-Genevois Cedex, France

Received 22 December 1998/Accepted 14 April 1999

A recombinant fusion protein (BBG2Na) comprising the central conserved domain of the respiratory syncytial virus subgroup A (RSV-A) (Long) G protein (residues 130 to 230) and an albumin binding domain of streptococcal protein G was shown previously to protect mouse upper (URT) and lower (LRT) respiratory tracts against intranasal RSV challenge (U. F. Power, H. Plotnicky-Gilquin, T. Huss, A. Robert, M. Trudel, S. Stahl, M. Uhlén, T. N. Nguyen, and H. Binz, Virology 230:155-166, 1997). Panels of monoclonal antibodies (MAbs) and synthetic peptides were generated to facilitate dissection of the structural elements of this domain implicated in protective efficacy. All MAbs recognized native RSV-A antigens, and five linear B-cell epitopes were identified; these mapped to residues 152 to 163, 165 to 172, 171 to 187 (two overlapping epitopes), and 196 to 204, thereby covering the highly conserved cysteine noose domain. Antibody passive-transfer and peptide immunization studies revealed that all epitopes were implicated in protection of the LRT, but not likely the URT, against RSV-A challenge. Pepscan analyses of anti-RSV-A and anti-BBG2Na murine polyclonal sera revealed lower-level epitope usage within the central conserved region in the former, suggesting diminished immunogenicity of the implicated epitopes in the context of the whole virus. However, Pepscan analyses of RSV-seropositive human sera revealed that all of the murine B-cell protective epitopes (protectopes) that mapped to the central conserved domain were recognized in man. Should these murine protectopes also be implicated in human LRT protection, their clustering around the highly conserved cysteine noose region will have important implications for the development of RSV vaccines.

* Corresponding author. Mailing address: Centre d'Immunologie Pierre Fabre, 5 Ave. Napolean II, BP 497, 74164 Saint-Julien-en-Genevois Cedex, France. Phone: (33) 450.35.35.49. Fax: (33) 450.35.35.90. E-mail: ultan.power{at}pierre-fabre.com.

Journal of Virology, July 1999, p. 5637-5645, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Miao, C., Radu, G. U., Caidi, H., Tripp, R. A., Anderson, L. J., Haynes, L. M. (2009). Treatment with respiratory syncytial virus G glycoprotein monoclonal antibody or F(ab')2 components mediates reduced pulmonary inflammation in mice. J. Gen. Virol. 90: 1119-1123 [Abstract] [Full Text]  
  • Mekseepralard, C., Toms, G. L., Routledge, E. G. (2006). Protection of mice against Human respiratory syncytial virus by wild-type and aglycosyl mouse-human chimaeric IgG antibodies to subgroup-conserved epitopes on the G glycoprotein.. J. Gen. Virol. 87: 1267-1273 [Abstract] [Full Text]  
  • Elliott, M. B., Pryharski, K. S., Yu, Q., Parks, C. L., Laughlin, T. S., Gupta, C. K., Lerch, R. A., Randolph, V. B., LaPierre, N. A., Dack, K. M. H., Hancock, G. E. (2004). Recombinant Respiratory Syncytial Viruses Lacking the C-Terminal Third of the Attachment (G) Protein Are Immunogenic and Attenuated In Vivo and In Vitro. J. Virol. 78: 5773-5783 [Abstract] [Full Text]  
  • Goetsch, L., Haeuw, J. F., Champion, T., Lacheny, C., N'Guyen, T., Beck, A., Corvaia, N. (2003). Identification of B- and T-Cell Epitopes of BB, a Carrier Protein Derived from the G Protein of Streptococcus Strain G148. CVI 10: 125-132 [Abstract] [Full Text]  
  • Plotnicky-Gilquin, H., Cyblat-Chanal, D., Aubry, J.-P., Champion, T., Beck, A., Nguyen, T., Bonnefoy, J.-Y., Corvaia, N. (2002). Gamma Interferon-Dependent Protection of the Mouse Upper Respiratory Tract following Parenteral Immunization with a Respiratory Syncytial Virus G Protein Fragment. J. Virol. 76: 10203-10210 [Abstract] [Full Text]  
  • Power, U. F., Huss, T., Michaud, V., Plotnicky-Gilquin, H., Bonnefoy, J.-Y., Nguyen, T. N. (2001). Differential Histopathology and Chemokine Gene Expression in Lung Tissues following Respiratory Syncytial Virus (RSV) Challenge of Formalin-Inactivated RSV- or BBG2Na-Immunized Mice. J. Virol. 75: 12421-12430 [Abstract] [Full Text]  
  • Goetsch, L., Gonzalez, A., Plotnicky-Gilquin, H., Haeuw, J. F., Aubry, J. P., Beck, A., Bonnefoy, J. Y., Corvaia, N. (2001). Targeting of Nasal Mucosa-Associated Antigen-Presenting Cells In Vivo with an Outer Membrane Protein A Derived from Klebsiella pneumoniae. Infect. Immun. 69: 6434-6444 [Abstract] [Full Text]  
  • Yip, Y. L., Smith, G., Koch, J., Dubel, S., Ward, R. L. (2001). Identification of Epitope Regions Recognized by Tumor Inhibitory and Stimulatory Anti-ErbB-2 Monoclonal Antibodies: Implications for Vaccine Design. J. Immunol. 166: 5271-5278 [Abstract] [Full Text]  
  • Plotnicky-Gilquin, H., Robert, A., Chevalet, L., Haeuw, J.-F., Beck, A., Bonnefoy, J.-Y., Brandt, C., Siegrist, C.-A., Nguyen, T. N., Power, U. F. (2000). CD4+ T-Cell-Mediated Antiviral Protection of the Upper Respiratory Tract in BALB/c Mice following Parenteral Immunization with a Recombinant Respiratory Syncytial Virus G Protein Fragment. J. Virol. 74: 3455-3463 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»