This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Woodberry, T.
Right arrow Articles by Suhrbier, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Woodberry, T.
Right arrow Articles by Suhrbier, A.

 Previous Article  |  Next Article 

Journal of Virology, July 1999, p. 5320-5325, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Immunogenicity of a Human Immunodeficiency Virus (HIV) Polytope Vaccine Containing Multiple HLA A2 HIV CD8+ Cytotoxic T-Cell Epitopes

T. Woodberry,1 J. Gardner,1 L. Mateo,1 D. Eisen,2 J. Medveczky,3 I. A. Ramshaw,3 S. A. Thomson,1 R. A. Ffrench,4 S. L. Elliott,1 H. Firat,5 F. A. Lemonnier,5 and A. Suhrbier1,*

Australian Centre for International & Tropical Health & Nutrition, Cooperative Research Centre for Vaccine Technology, Queensland Institute of Medical Research,1 and Infectious Diseases Unit, Royal Brisbane Hospital,2 Brisbane, and Division of Immunology and Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra,3 and Paediatric Research Laboratories, Sydney Children's Hospital, Randwick,4 Australia, and Département SIDA-Rétrovirus, Unité d'Immunite Cellulaire Antivirale, Institut Pasteur, Paris, France5

Received 14 December 1998/Accepted 18 March 1999

Compelling evidence now suggests that alpha beta CD8 cytotoxic T lymphocytes (CTL) have an important role in preventing human immunodeficiency virus (HIV) infection and/or slowing progression to AIDS. Here, we describe an HIV type 1 CTL polyepitope, or polytope, vaccine comprising seven contiguous minimal HLA A2-restricted CD8 CTL epitopes conjoined in a single artificial construct. Epitope-specific CTL lines derived from HIV-infected individuals were able to recognize every epitope within the construct, and HLA A2-transgenic mice immunized with a recombinant virus vaccine coding for the HIV polytope also generated CTL specific for different epitopes. Each epitope in the polytope construct was therefore processed and presented, illustrating the feasibility of the polytope approach for HIV vaccine design. By simultaneously inducing CTL specific for different epitopes, an HIV polytope vaccine might generate activity against multiple challenge isolates and/or preempt the formation of CTL escape mutants.

* Corresponding author. Mailing address: Queensland Institute of Medical Research, Post Office Royal Brisbane Hospital, Brisbane, Qld. 4029, Australia. Phone: 61-7-33620415. Fax: 61-7-33620107. E-mail: andreasS{at}qimr.edu.au.

Journal of Virology, July 1999, p. 5320-5325, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hu, J., Peng, X., Schell, T. D., Budgeon, L. R., Cladel, N. M., Christensen, N. D. (2006). An HLA-A2.1-Transgenic Rabbit Model to Study Immunity to Papillomavirus Infection. J. Immunol. 177: 8037-8045 [Abstract] [Full Text]  
  • Rueda, P., Moron, G., Sarraseca, J., Leclerc, C., Casal, J. I. (2004). Influence of flanking sequences on presentation efficiency of a CD8+ cytotoxic T-cell epitope delivered by parvovirus-like particles. J. Gen. Virol. 85: 563-572 [Abstract] [Full Text]  
  • Daftarian, P., Ali, S., Sharan, R., Lacey, S. F., La Rosa, C., Longmate, J., Buck, C., Siliciano, R. F., Diamond, D. J. (2003). Immunization with Th-CTL Fusion Peptide and Cytosine-Phosphate-Guanine DNA in Transgenic HLA-A2 Mice Induces Recognition of HIV-Infected T Cells and Clears Vaccinia Virus Challenge. J. Immunol. 171: 4028-4039 [Abstract] [Full Text]  
  • Boissonnas, A., Bonduelle, O., Antzack, A., Lone, Y.-C., Gache, C., Debre, P., Autran, B., Combadiere, B. (2002). In Vivo Priming Of HIV-Specific CTLs Determines Selective Cross-Reactive Immune Responses Against Poorly Immunogenic HIV-Natural Variants. J. Immunol. 169: 3694-3699 [Abstract] [Full Text]  
  • VanLith, M. L., Kohlgraf, K. G., Sivinski, C. L., Tempero, R. M., Hollingsworth, M. A. (2002). MUC1-specific anti-tumor responses: molecular requirements for CD4-mediated responses. Int Immunol 14: 873-882 [Abstract] [Full Text]  
  • Brinster, C., Chen, M., Boucreux, D., Paranhos-Baccala, G., Liljestrom, P., Lemmonier, F., Inchauspe, G. (2002). Hepatitis C virus non-structural protein 3-specific cellular immune responses following single or combined immunization with DNA or recombinant Semliki Forest virus particles. J. Gen. Virol. 83: 369-381 [Abstract] [Full Text]  
  • Smith, S. G., Patel, P. M., Porte, J., Selby, P. J., Jackson, A. M. (2001). Human Dendritic Cells Genetically Engineered to Express a Melanoma Polyepitope DNA Vaccine Induce Multiple Cytotoxic T-Cell Responses. Clin. Cancer Res. 7: 4253-4261 [Abstract] [Full Text]  
  • Ferko, B., Stasakova, J., Sereinig, S., Romanova, J., Katinger, D., Niebler, B., Katinger, H., Egorov, A. (2001). Hyperattenuated Recombinant Influenza A Virus Nonstructural-Protein-Encoding Vectors Induce Human Immunodeficiency Virus Type 1 Nef-Specific Systemic and Mucosal Immune Responses in Mice. J. Virol. 75: 8899-8908 [Abstract] [Full Text]  
  • Allen, T. M., Mothé, B. R., Sidney, J., Jing, P., Dzuris, J. L., Liebl, M. E., Vogel, T. U., O'Connor, D. H., Wang, X., Wussow, M. C., Thomson, J. A., Altman, J. D., Watkins, D. I., Sette, A. (2001). CD8+ Lymphocytes from Simian Immunodeficiency Virus-Infected Rhesus Macaques Recognize 14 Different Epitopes Bound by the Major Histocompatibility Complex Class I Molecule Mamu-A*01: Implications for Vaccine Design and Testing. J. Virol. 75: 738-749 [Abstract] [Full Text]  
  • Loirat, D., Lemonnier, F. A., Michel, M.-L. (2000). Multiepitopic HLA-A*0201-Restricted Immune Response Against Hepatitis B Surface Antigen After DNA-Based Immunization. J. Immunol. 165: 4748-4755 [Abstract] [Full Text]  
  • Goulder, P. J. R., Brander, C., Annamalai, K., Mngqundaniso, N., Govender, U., Tang, Y., He, S., Hartman, K. E., O'Callaghan, C. A., Ogg, G. S., Altfeld, M. A., Rosenberg, E. S., Cao, H., Kalams, S. A., Hammond, M., Bunce, M., Pelton, S. I., Burchett, S. A., McIntosh, K., Coovadia, H. M., Walker, B. D. (2000). Differential Narrow Focusing of Immunodominant Human Immunodeficiency Virus Gag-Specific Cytotoxic T-Lymphocyte Responses in Infected African and Caucasoid Adults and Children. J. Virol. 74: 5679-5690 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»