Human Erythrocyte Glycosphingolipids as Alternative Cofactors for Human Immunodeficiency Virus Type 1 (HIV-1) Entry: Evidence for CD4-Induced Interactions between HIV-1 gp120 and Reconstituted Membrane Microdomains of Glycosphingolipids (Gb3 and GM3)

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Journal of Virology, June 1999, p. 5244-5248, Vol. 73, No. 6
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Human Erythrocyte Glycosphingolipids as Alternative Cofactors for Human Immunodeficiency Virus Type 1 (HIV-1) Entry: Evidence for CD4-Induced Interactions between HIV-1 gp120 and Reconstituted Membrane Microdomains of Glycosphingolipids (Gb3 and GM3)

Djilali Hammache,¹ Nouara Yahi,² Marc Maresca,¹ Gérard Piéroni,³ and Jacques Fantini¹^,^*

Laboratoire de Biochimie et Biologie de la Nutrition, ESA-CNRS 6033, Faculté des Sciences de St Jérôme, 13397 Marseille Cedex 20,¹ Laboratoire de Virologie, UF SIDA, Hôpital de la Timone, 13005 Marseille,² and INSERM U130, 13009 Marseille,³ France

Received 26 October 1998/Accepted 5 March 1999

Glycosphingolipids from human erythrocytes mediate CD4-dependent fusion with cells expressing human immunodeficiency virustype 1 (HIV-1) envelope glycoproteins. To identify the glycosphingolipid(s)which participates in the fusion process, we have analyzed theinteraction of HIV-1 gp120 (X4 and R5X4 isolates) with reconstitutedmembrane microdomains of human erythrocyte glycosphingolipids.We identified globotriaosylceramide (Gb3) and ganglioside GM3as the main glycosphingolipids recognized by gp120. In the presenceof CD4, Gb3 interacted preferentially with the X4 gp120, whereasGM3 interacted exclusively with the R5X4 gp120. These data suggestthat glycosphingolipid microdomains are required in CD4-dependentfusion and that Gb3 and/or GM3 may function as alternative entrycofactors for selected HIV-1isolates.

^* Corresponding author. Mailing address: Laboratoire de Biochimie et Biologie de la Nutrition, ESA-CNRS 6033, Faculté des Sciencesde St Jérôme, 13397 Marseille cedex 20, France. Phone: 33 491-288-761.Fax: 33 491-288-440. E-mail: JACQUES.FANTINI{at}LBBN.u-3mrs.fr.

Journal of Virology, June 1999, p. 5244-5248, Vol. 73, No. 6
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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