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Journal of Virology, June 1999, p. 5181-5185, Vol. 73, No. 6
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Epstein-Barr Virus Encodes a Novel Homolog of the bcl-2 Oncogene That Inhibits Apoptosis and Associates with Bax and Bak

William L. Marshall,1,* Ching Yim,1 Erik Gustafson,2 Thomas Graf,1 David R. Sage,2 Katherine Hanify,1 Louisa Williams,1 Joyce Fingeroth,2 and Robert W. Finberg1

Division of Infectious Disease, Department of Adult Oncology, Dana-Farber Cancer Institute,1 and Department of Infectious Disease and Experimental Medicine, Beth Israel-Deaconess Medical Center, 2 Boston, Massachusetts 02115

Received 23 February 1999/Accepted 2 March 1999

The sequenced gammaherpesviruses each contain a single viral bcl-2 homolog (v-bcl-2) which may encode a protein that functions in preventing the apoptotic death of virus-infected cells. Epstein-Barr virus (EBV), a gammaherpesvirus associated with several lymphoid and epithelial malignancies, encodes the v-Bcl-2 homolog BHRF1. In this report the previously uncharacterized BALF1 open reading frame in EBV is identified as having significant sequence similarity to other v-bcl-2 homologs and cellular bcl-2. Transfection of cells with a BALF1 cDNA conferred apoptosis resistance. Furthermore, a recombinant green fluorescent protein-BALF1 fusion protein suppressed apoptosis and associated with Bax and Bak. These results indicate that EBV encodes a second functional v-bcl-2.

* Corresponding author. Mailing address: Department of Adult Oncology, Dana-Farber Cancer Institute, Boston, MA 02115. Phone: (617) 632-4273. Fax: (617) 632-4257. E-mail: wmarshall{at}partners.org.

Journal of Virology, June 1999, p. 5181-5185, Vol. 73, No. 6
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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