This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jan, J.-T.
Right arrow Articles by Griffin, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jan, J.-T.
Right arrow Articles by Griffin, D. E.

 Previous Article  |  Next Article 

Journal of Virology, June 1999, p. 4919-4924, Vol. 73, No. 6
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Characterization of a Chinese Hamster Ovary Cell Line Developed by Retroviral Insertional Mutagenesis That Is Resistant to Sindbis Virus Infection

Jia-Tsrong Jan,dagger Andrew P. Byrnes, and Diane E. Griffin*

Department of Molecular Microbiology and Immunology, Johns Hopkins School of Public Health, Baltimore, Maryland 21205

Received 22 December 1998/Accepted 16 March 1999

The alphavirus Sindbis virus (SV) has a wide host range and infects many types of cultured cells in vitro. The outcome of infection is dependent on the strain of virus used for infection and the properties of the cells infected. To identify cellular determinants of susceptibility to SV infection we mutagenized Chinese hamster ovary (CHO) cells by retroviral insertion with a vector containing the neomycin resistance gene that allowed selection for integration into transcriptionally active genes. Cells were then selected for survival after infection with SV. The most resistant cell line (CHO-18.4m) exhibited delayed virus replication and virus-induced cell death, had a single retroviral insertion, and was defective in SV binding to the cell surface. Further analysis revealed that CHO-18.4m cells were deficient in the expression of the sulfated glycosaminoglycans heparan sulfate and chondroitin sulfate. This further confirms the importance of heparan sulfate as an attachment molecule for SV in vitro and demonstrates the usefulness of this technique for identifying cellular genes that are important for virus replication.

* Corresponding author. Mailing address: Department of Molecular Microbiology and Immunology, Johns Hopkins School of Public Health, 615 N. Wolfe St., Rm. E5132, Baltimore, MD 21205. Phone: (410) 955-3459. Fax: (410) 955-0105. E-mail: dgriffin{at}jhsph.edu.

dagger Present address: National Defense Medical Center, Institute of Preventive Medicine, Taipei, Taiwan, Republic of China.

Journal of Virology, June 1999, p. 4919-4924, Vol. 73, No. 6
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Knight, R. L., Schultz, K. L. W., Kent, R. J., Venkatesan, M., Griffin, D. E. (2009). Role of N-Linked Glycosylation for Sindbis Virus Infection and Replication in Vertebrate and Invertebrate Systems. J. Virol. 83: 5640-5647 [Abstract] [Full Text]  
  • Stephens, R. S., Poteralski, J. M., Olinger, L. (2006). Interaction of Chlamydia trachomatis with Mammalian Cells Is Independent of Host Cell Surface Heparan Sulfate Glycosaminoglycans. Infect. Immun. 74: 1795-1799 [Abstract] [Full Text]  
  • Heil, M. L., Albee, A., Strauss, J. H., Kuhn, R. J. (2001). An Amino Acid Substitution in the Coding Region of the E2 Glycoprotein Adapts Ross River Virus To Utilize Heparan Sulfate as an Attachment Moiety. J. Virol. 75: 6303-6309 [Abstract] [Full Text]  
  • Mandl, C. W., Kroschewski, H., Allison, S. L., Kofler, R., Holzmann, H., Meixner, T., Heinz, F. X. (2001). Adaptation of Tick-Borne Encephalitis Virus to BHK-21 Cells Results in the Formation of Multiple Heparan Sulfate Binding Sites in the Envelope Protein and Attenuation In Vivo. J. Virol. 75: 5627-5637 [Abstract] [Full Text]  
  • Jan, J.-T., Chen, B.-H., Ma, S.-H., Liu, C.-I, Tsai, H.-P., Wu, H.-C., Jiang, S.-Y., Yang, K.-D., Shaio, M.-F. (2000). Potential Dengue Virus-Triggered Apoptotic Pathway in Human Neuroblastoma Cells: Arachidonic Acid, Superoxide Anion, and NF-kappa B Are Sequentially Involved. J. Virol. 74: 8680-8691 [Abstract] [Full Text]  
  • Byrnes, A. P., Griffin, D. E. (2000). Large-Plaque Mutants of Sindbis Virus Show Reduced Binding to Heparan Sulfate, Heightened Viremia, and Slower Clearance from the Circulation. J. Virol. 74: 644-651 [Abstract] [Full Text]  
  • Jan, J.-T., Griffin, D. E. (1999). Induction of Apoptosis by Sindbis Virus Occurs at Cell Entry and Does Not Require Virus Replication. J. Virol. 73: 10296-10302 [Abstract] [Full Text]  
  • Klimstra, W. B., Ryman, K. D., Bernard, K. A., Nguyen, K. B., Biron, C. A., Johnston, R. E. (1999). Infection of Neonatal Mice with Sindbis Virus Results in a Systemic Inflammatory Response Syndrome. J. Virol. 73: 10387-10398 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»