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Journal of Virology, May 1999, p. 4475-4480, Vol. 73, No. 5
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Monoclonal Antibodies against the Minimal DNA-Binding Domain in the Carboxyl-Terminal Region of Human Immunodeficiency Virus Type 1 Integrase

Tetsuya Ishikawa,1,2 Nobuo Okui,1,3 Noriko Kobayashi,1 Ryuta Sakuma,1 Tadaichi Kitamura,3 and Yoshihiro Kitamura1,*

Division of Molecular Genetics, National Institute of Infectious Diseases, Musashimurayama,1 and Department of Urology, Faculty of Medicine, University of Tokyo, Bunkyo-ku,3 Tokyo, and Institute of Biomedical Science, Terumo Corporation R. & D. Center, Ashigarakami-gun, Kanagawa,2 Japan

Received 21 October 1998/Accepted 13 February 1999

Integrase of human immunodeficiency virus type 1 (HIVIN) consists of 288 amino acids, and its minimum DNA-binding domain (MDBD) (amino acids [aa] 220 to 270) is required for the integration reaction. We produced and characterized four murine monoclonal antibodies (MAbs) to the MDBD of HIVIN (strain LAI). Immunoblot and enzyme-linked immunosorbent assays with truncated HIVINs showed that those MAbs recognized sequential epitopes within the MDBD (aa 228 to 236, 237 to 252, 253 to 261, and 262 to 270). Their binding to HIVIN inhibited terminal cleavage and strand transfer activities but not disintegration activity in vitro. This collection of MAbs is useful for studying the structure and function of the MDBD by complementing mutational analyses and other biochemical studies.

* Corresponding author. Mailing address: Division of Molecular Genetics, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan. Phone: 81-425-61-0771, ext. 370. Fax: 81-425-67-5632. E-mail: yochan{at}nih.go.jp.

Journal of Virology, May 1999, p. 4475-4480, Vol. 73, No. 5
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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