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Journal of Virology, May 1999, p. 4452-4455, Vol. 73, No. 5
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Immunization with Potato Plants Expressing VP60 Protein Protects against Rabbit Hemorrhagic Disease Virus

S. Castañón,1 M. S. Marín,2 J. M. Martín-Alonso,2 J. A. Boga,2 R. Casais,2 J. M. Humara,1 R. J. Ordás,1 and F. Parra2,*

Departamento de Biología de Organismos y Sistemas1 and Departamento de Bioquímica y Biología Molecular,2 Instituto Universitario de Biotecnología de Asturias (CSIC), Universidad de Oviedo, 33006 Oviedo, Spain

Received 26 October 1998/Accepted 9 February 1999

The major structural protein VP60 of rabbit hemorrhagic disease virus (RHDV) has been produced in transgenic potato plants under the control of a cauliflower mosaic virus 35S promoter or a modified 35S promoter that included two copies of a strong transcriptional enhancer. Both types of promoters allowed the production of specific mRNAs and detectable levels of recombinant VP60, which were higher for the constructs carrying the modified 35S promoter. Rabbits immunized with leaf extracts from plants carrying this modified 35S promoter showed high anti-VP60 antibody titers and were fully protected against the hemorrhagic disease.

* Corresponding author. Mailing address: Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain. Phone: 34 985 103563. Fax: 34 985 103157. E-mail: parra{at}biosun.quimica.uniovi.es.

Journal of Virology, May 1999, p. 4452-4455, Vol. 73, No. 5
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.