This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Asada, H.
Right arrow Articles by Blauvelt, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Asada, H.
Right arrow Articles by Blauvelt, A.

 Previous Article  |  Next Article 

Journal of Virology, May 1999, p. 4019-4028, Vol. 73, No. 5
0022-538X/99/$04.00+0

Human Herpesvirus 6 Infects Dendritic Cells and Suppresses Human Immunodeficiency Virus Type 1 Replication in Coinfected Cultures

Hideo Asada,1 Vera Klaus-Kovtun,1 Hana Golding,2 Stephen I. Katz,1 and Andrew Blauvelt1,*

Dermatology Branch, National Cancer Institute,1 and Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration,2 Bethesda, Maryland 20892

Received 23 October 1998/Accepted 1 February 1999

Human herpesvirus 6 (HHV-6) has been implicated as a cofactor in the progressive loss of CD4+ T cells observed in AIDS patients. Because dendritic cells (DC) play an important role in the immunopathogenesis of human immunodeficiency virus (HIV) disease, we studied the infection of DC by HHV-6 and coinfection of DC by HHV-6 and HIV. Purified immature DC (derived from adherent peripheral blood mononuclear cells in the presence of granulocyte-macrophage colony-stimulating factor and interleukin-4) could be infected with HHV-6, as determined by PCR analyses, intracellular monoclonal antibody staining, and presence of virus in culture supernatants. However, HHV-6-infected DC demonstrated neither cytopathic changes nor functional defects. Interestingly, HHV-6 markedly suppressed HIV replication and syncytium formation in coinfected DC cultures. This HHV-6-mediated anti-HIV effect was DC specific, occurred when HHV-6 was added either before or after HIV, and was not due to decreased surface expression or function of CD4, CXCR4, or CCR5. Conversely, HIV had no demonstrable effect on HHV-6 replication. These findings suggest that HHV-6 may protect DC from HIV-induced cytopathicity in AIDS patients. We also demonstrate that interactions between HIV and herpesviruses are complex and that the observable outcome of dual infection is dependent on the target cell type.

* Corresponding author. Mailing address: Dermatology Branch, National Cancer Institute, Building 10/Room 12N238, 10 Center Dr. MSC 1908, Bethesda, MD 20892-1908. Phone: (301) 402-4167. Fax: (301) 402-1439. E-mail: Andrew_Blauvelt{at}nih.gov.

Journal of Virology, May 1999, p. 4019-4028, Vol. 73, No. 5
0022-538X/99/$04.00+0


This article has been cited by other articles:

  • De Bolle, L., Naesens, L., De Clercq, E. (2005). Update on Human Herpesvirus 6 Biology, Clinical Features, and Therapy. Clin. Microbiol. Rev. 18: 217-245 [Abstract] [Full Text]  
  • Borenstein, R., Singer, O., Moseri, A., Frenkel, N. (2004). Use of Amplicon-6 Vectors Derived from Human Herpesvirus 6 for Efficient Expression of Membrane-Associated and -Secreted Proteins in T Cells. J. Virol. 78: 4730-4743 [Abstract] [Full Text]  
  • Dockrell, D.H. (2003). Human herpesvirus 6: molecular biology and clinical features. J Med Microbiol 52: 5-18 [Abstract] [Full Text]  
  • Kakimoto, M., Hasegawa, A., Fujita, S., Yasukawa, M. (2002). Phenotypic and Functional Alterations of Dendritic Cells Induced by Human Herpesvirus 6 Infection. J. Virol. 76: 10338-10345 [Abstract] [Full Text]  
  • Lawn, S. D., Butera, S. T., Folks, T. M. (2001). Contribution of Immune Activation to the Pathogenesis and Transmission of Human Immunodeficiency Virus Type 1 Infection. Clin. Microbiol. Rev. 14: 753-777 [Abstract] [Full Text]  
  • Xiang, J., Wunschmann, S., Diekema, D. J., Klinzman, D., Patrick, K. D., George, S. L., Stapleton, J. T. (2001). Effect of Coinfection with GB Virus C on Survival among Patients with HIV Infection. NEJM 345: 707-714 [Abstract] [Full Text]  
  • Abendroth, A., Morrow, G., Cunningham, A. L., Slobedman, B. (2001). Varicella-Zoster Virus Infection of Human Dendritic Cells and Transmission to T Cells: Implications for Virus Dissemination in the Host. J. Virol. 75: 6183-6192 [Abstract] [Full Text]  
  • Pinto, L. A., Blazevic, V., Patterson, B. K., Mac Trubey, C., Dolan, M. J., Shearer, G. M. (2000). Inhibition of Human Immunodeficiency Virus Type 1 Replication prior to Reverse Transcription by Influenza Virus Stimulation. J. Virol. 74: 4505-4511 [Abstract] [Full Text]  
  • Huang, L.-M., Chao, M.-F., Chen, M.-Y., Shih, H.-m., Chiang, Y.-P., Chuang, C.-Y., Lee, C.-Y. (2001). Reciprocal Regulatory Interaction between Human Herpesvirus 8 and Human Immunodeficiency Virus Type 1. J. Biol. Chem. 276: 13427-13432 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»