This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huber, M. T.
Right arrow Articles by Compton, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huber, M. T.
Right arrow Articles by Compton, T.

 Previous Article  |  Next Article 

Journal of Virology, May 1999, p. 3886-3892, Vol. 73, No. 5
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Intracellular Formation and Processing of the Heterotrimeric gH-gL-gO (gCIII) Glycoprotein Envelope Complex of Human Cytomegalovirus

Mary T. Huber and Teresa Compton*

Program in Cellular and Molecular Biology and Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin 53706

Received 7 December 1998/Accepted 1 February 1999

The human cytomegalovirus (HCMV) gCIII complex contains glycoprotein H (gH; gpUL75), glycoprotein L (gL; gpUL115), and glycoprotein O (gO; gpUL74). To examine how gH, gL, and gO interact within HCMV-infected cells to assemble the tripartite complex, pulse-chase experiments were performed. These analyses demonstrated that gH and gL associate by the end of the pulse period to form a disulfide dependent gH-gL complex. Subsequently, the gH-gL complex interacts with a 100-kDa precursor form of gO to form a 220-kDa precursor of the mature gH-gL-gO complex that contains a 125-kDa form of gO. The 220-kDa precursor complex (pgCIII) was sensitive to treatment with endoglycosidase H (endo H), while the mature gCIII complex was essentially resistant to digestion with this enzyme, suggesting that formation of pgCIII complex occurs in the endoplasmic reticulum (ER) and is processed to mature gH-gL-gO (gCIII) in a post-ER compartment. While the N-linked glycans on the 100-kDa form of gO were modified to endo H-resistant states as the 125-kDa gO formed, additional posttranslational modifications were detected on gO. These processing alterations were non-N-linked oligosaccharide modifications that could not be accounted for by phosphorylation or by O-glycosylation of the type sensitive to O-glycanase. Of gH, gL, gO, and the various complexes that they form, only the mature form of the complex was detectable at the infected cell membrane, as judged by surface biotinylation studies.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706. Phone: (608) 262-1474. Fax: (608) 262-8418. E-mail: tcompton{at}facstaff.wisc.edu.

Journal of Virology, May 1999, p. 3886-3892, Vol. 73, No. 5
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ryckman, B. J., Rainish, B. L., Chase, M. C., Borton, J. A., Nelson, J. A., Jarvis, M. A., Johnson, D. C. (2008). Characterization of the Human Cytomegalovirus gH/gL/UL128-131 Complex That Mediates Entry into Epithelial and Endothelial Cells. J. Virol. 82: 60-70 [Abstract] [Full Text]  
  • English, E. P., Chumanov, R. S., Gellman, S. H., Compton, T. (2006). Rational Development of beta-Peptide Inhibitors of Human Cytomegalovirus Entry. J. Biol. Chem. 281: 2661-2667 [Abstract] [Full Text]  
  • Wang, D., Shenk, T. (2005). Human cytomegalovirus virion protein complex required for epithelial and endothelial cell tropism. Proc. Natl. Acad. Sci. USA 102: 18153-18158 [Abstract] [Full Text]  
  • Kinzler, E. R., Compton, T. (2005). Characterization of Human Cytomegalovirus Glycoprotein-Induced Cell-Cell Fusion. J. Virol. 79: 7827-7837 [Abstract] [Full Text]  
  • Varnum, S. M., Streblow, D. N., Monroe, M. E., Smith, P., Auberry, K. J., Pasa-Tolic, L., Wang, D., Camp, D. G. II, Rodland, K., Wiley, S., Britt, W., Shenk, T., Smith, R. D., Nelson, J. A. (2004). Identification of Proteins in Human Cytomegalovirus (HCMV) Particles: the HCMV Proteome. J. Virol. 78: 10960-10966 [Abstract] [Full Text]  
  • Kattenhorn, L. M., Mills, R., Wagner, M., Lomsadze, A., Makeev, V., Borodovsky, M., Ploegh, H. L., Kessler, B. M. (2004). Identification of Proteins Associated with Murine Cytomegalovirus Virions. J. Virol. 78: 11187-11197 [Abstract] [Full Text]  
  • Akkapaiboon, P., Mori, Y., Sadaoka, T., Yonemoto, S., Yamanishi, K. (2004). Intracellular Processing of Human Herpesvirus 6 Glycoproteins Q1 and Q2 into Tetrameric Complexes Expressed on the Viral Envelope. J. Virol. 78: 7969-7983 [Abstract] [Full Text]  
  • Mori, Y., Akkapaiboon, P., Yonemoto, S., Koike, M., Takemoto, M., Sadaoka, T., Sasamoto, Y., Konishi, S., Uchiyama, Y., Yamanishi, K. (2004). Discovery of a Second Form of Tripartite Complex Containing gH-gL of Human Herpesvirus 6 and Observations on CD46. J. Virol. 78: 4609-4616 [Abstract] [Full Text]  
  • Mori, Y., Akkapaiboon, P., Yang, X., Yamanishi, K. (2003). The Human Herpesvirus 6 U100 Gene Product Is the Third Component of the gH-gL Glycoprotein Complex on the Viral Envelope. J. Virol. 77: 2452-2458 [Abstract] [Full Text]  
  • Rasmussen, L., Geissler, A., Cowan, C., Chase, A., Winters, M. (2002). The Genes Encoding the gCIII Complex of Human Cytomegalovirus Exist in Highly Diverse Combinations in Clinical Isolates. J. Virol. 76: 10841-10848 [Abstract] [Full Text]  
  • Theiler, R. N., Compton, T. (2002). Distinct Glycoprotein O Complexes Arise in a Post-Golgi Compartment of Cytomegalovirus-Infected Cells. J. Virol. 76: 2890-2898 [Abstract] [Full Text]  
  • Theiler, R. N., Compton, T. (2001). Characterization of the Signal Peptide Processing and Membrane Association of Human Cytomegalovirus Glycoprotein O. J. Biol. Chem. 276: 39226-39231 [Abstract] [Full Text]  
  • Bahr, U., Darai, G. (2001). Analysis and Characterization of the Complete Genome of Tupaia (Tree Shrew) Herpesvirus. J. Virol. 75: 4854-4870 [Abstract] [Full Text]  
  • Mach, M., Kropff, B., Dal Monte, P., Britt, W. (2000). Complex Formation by Human Cytomegalovirus Glycoproteins M (gpUL100) and N (gpUL73). J. Virol. 74: 11881-11892 [Abstract] [Full Text]  
  • Wang, S.-K., Duh, C.-Y., Chang, T.-T. (2000). Cloning and identification of regulatory gene UL76 of human cytomegalovirus. J. Gen. Virol. 81: 2407-2416 [Abstract] [Full Text]  
  • Hobom, U., Brune, W., Messerle, M., Hahn, G., Koszinowski, U. H. (2000). Fast Screening Procedures for Random Transposon Libraries of Cloned Herpesvirus Genomes: Mutational Analysis of Human Cytomegalovirus Envelope Glycoprotein Genes. J. Virol. 74: 7720-7729 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»