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Journal of Virology, April 1999, p. 3449-3454, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Human Immunodeficiency Virus Type 1 Derived from Cocultures of Immature Dendritic Cells with Autologous T Cells Carries T-Cell-Specific Molecules on Its Surface and Is Highly Infectious

Ines Frank,1 Laco Kacani,1 Heribert Stoiber,1 Hella Stössel,2 Martin Spruth,1 Franz Steindl,3 Nikolaus Romani,2 and Manfred P. Dierich1,*

Institute for Hygiene and Ludwig Boltzmann Institute for AIDS Research1 and Department of Dermatology,2 University of Innsbruck, Innsbruck, and Institute for Applied Microbiology, University of Agriculture, Vienna,3 Austria

Received 16 September 1998/Accepted 5 January 1999

During the budding process, human immunodeficiency virus type 1 (HIV-1) acquires cell surface molecules; thus, the viral surface of HIV-1 reflects the antigenic pattern of the host cell. To determine the source of HIV-1 released from cocultures of dendritic cells (DC) with T cells, immature DC (imDC), mature DC (mDC), T cells, and their cocultures were infected with different HIV-1 isolates. The macrophage-tropic HIV-1 isolate Ba-L allowed viral replication in both imDC and mDC, whereas the T-cell-line-tropic primary isolate PI21 replicated in mDC only. By a virus capture assay, HIV-1 was shown to carry a T-cell- or DC-specific cell surface pattern after production by T cells or DC, respectively. Upon cocultivation of HIV-1-pulsed DC with T cells, HIV-1 exclusively displayed a typical T-cell pattern. Additionally, functional analysis revealed that HIV-1 released from imDC-T-cell cocultures was more infectious than HIV-1 derived from mDC-T-cell cocultures and from cultures of DC, T cells, or peripheral blood mononuclear cells alone. Therefore, we conclude that the interaction of HIV-1-pulsed imDC with T cells in vivo might generate highly infectious virus which primarily originates from T cells.

* Corresponding author. Mailing address: Institut für Hygiene, Leopold-Franzens-Universität, Fritz-Pregl-Str. 3/III, A-6020 Innsbruck, Austria. Phone: 43-512-507-3401. Fax: 43-512-507-2870. E-mail: hygiene{at}uibk.ac.at.

Journal of Virology, April 1999, p. 3449-3454, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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