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Journal of Virology, April 1999, p. 2798-2802, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Single Amino Acid in the Adenovirus Type 37 Fiber Confers Binding to Human Conjunctival Cellsdagger

Shuang Huang, Vijay Reddy, Nabarun Dasgupta, and Glen R. Nemerow*

Departments of Immunology and Molecular Biology, The Scripps Research Institute, La Jolla, California

Received 18 September 1998/Accepted 15 December 1998

A 46-kDa receptor, coxsackievirus-adenovirus (Ad) receptor (CAR), mediates cell attachment of a number of different Ad serotypes; however, not all Ad serotypes utilize this receptor for infection. Moreover, the precise amino acid sequences in the Ad fiber protein that mediate cell attachment have yet to be identified. We investigated the interaction of subgroup D Ads with human ocular cells. Ad serotype 37 (Ad37), a virus associated with epidemic keratoconjunctivitis, but not a closely related virus serotype, Ad19p, exhibited preferential binding to and infection of human conjunctival cells. A single amino acid substitution in the Ad19p fiber distal domain (knob), Glu240 to Lys, conferred binding to conjunctival cells, while the reverse substitution in the Ad37 fiber abrogated cell binding. These findings provide new information on the fiber sequences that regulate Ad host cell tropism.

* Corresponding author. Mailing address: Department of Immunology, IMM19, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (619) 784-8072. Fax: (619) 784-8472. E-mail: gnemerow{at}scripps.edu.

dagger Manuscript no. 11946-IMM of The Scripps Research Institute.

Journal of Virology, April 1999, p. 2798-2802, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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