This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Simon, J. H. M.
Right arrow Articles by Malim, M. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Simon, J. H. M.
Right arrow Articles by Malim, M. H.

 Previous Article  |  Next Article 

Journal of Virology, April 1999, p. 2667-2674, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Vif and the p55Gag Polyprotein of Human Immunodeficiency Virus Type 1 Are Present in Colocalizing Membrane-Free Cytoplasmic Complexes

James H. M. Simon,1 Elise A. Carpenter,2 Ron A. M. Fouchier,2 and Michael H. Malim1,2,3,*

Howard Hughes Medical Institute2 and Departments of Microbiology1 and Medicine,3 University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6148

Received 30 July 1998/Accepted 9 December 1998

The Vif protein of human immunodeficiency virus type 1 (HIV-1) is a potent regulator of viral infectivity. Current data posit that Vif functions late in replication to modulate assembly, budding, and/or maturation. Consistent with this model, earlier indirect immunofluorescence analyses of HIV-1-infected cells demonstrated that Vif and Gag colocalize to a substantial degree (J. H. M. Simon, R. A. M. Fouchier, T. E. Southerling, C. B. Guerra, C. K. Grant, and M. H. Malim, J. Virol. 71:5259-5267, 1997). Here, we describe a series of subcellular fractionation studies which indicate that Vif and the p55Gag polyprotein are present in membrane-free cytoplasmic complexes that copurify in sucrose density gradients and are stable in nonionic detergents. Both Vif and Gag are targeted to these complexes independent of each other, and their association with them appears to be mediated by protein-protein interactions. We propose that these complexes may represent viral assembly intermediates and that Vif is appropriately localized to influence the final stages of the viral life cycle and, therefore, the infectivity of progeny virions.

* Corresponding author. Mailing address: Departments of Microbiology and Medicine, University of Pennsylvania School of Medicine, Clinical Research Bldg., Room 347B, 415 Curie Blvd., Philadelphia, PA 19104-6148. Phone: (215) 573-3493. Fax: (215) 573-2172. E-mail: malim{at}mail.med.upenn.edu.

Journal of Virology, April 1999, p. 2667-2674, Vol. 73, No. 4
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Henriet, S., Mercenne, G., Bernacchi, S., Paillart, J.-C., Marquet, R. (2009). Tumultuous Relationship between the Human Immunodeficiency Virus Type 1 Viral Infectivity Factor (Vif) and the Human APOBEC-3G and APOBEC-3F Restriction Factors. Microbiol. Mol. Biol. Rev. 73: 211-232 [Abstract] [Full Text]  
  • Blay, W. M., Kasprzyk, T., Misher, L., Richardson, B. A., Haigwood, N. L. (2007). Mutations in Envelope gp120 Can Impact Proteolytic Processing of the gp160 Precursor and Thereby Affect Neutralization Sensitivity of Human Immunodeficiency Virus Type 1 Pseudoviruses. J. Virol. 81: 13037-13049 [Abstract] [Full Text]  
  • Bernacchi, S., Henriet, S., Dumas, P., Paillart, J.-C., Marquet, R. (2007). RNA and DNA Binding Properties of HIV-1 Vif Protein: A FLUORESCENCE STUDY. J. Biol. Chem. 282: 26361-26368 [Abstract] [Full Text]  
  • Jager, S., Gottwein, E., Krausslich, H.-G. (2007). Ubiquitination of Human Immunodeficiency Virus Type 1 Gag Is Highly Dependent on Gag Membrane Association. J. Virol. 81: 9193-9201 [Abstract] [Full Text]  
  • Henriet, S., Sinck, L., Bec, G., Gorelick, R. J., Marquet, R., Paillart, J.-C. (2007). Vif is a RNA chaperone that could temporally regulate RNA dimerization and the early steps of HIV-1 reverse transcription. Nucleic Acids Res 35: 5141-5153 [Abstract] [Full Text]  
  • Scheifele, L. Z., Ryan, E. P., Parent, L. J. (2005). Detailed Mapping of the Nuclear Export Signal in the Rous Sarcoma Virus Gag Protein. J. Virol. 79: 8732-8741 [Abstract] [Full Text]  
  • Ribeiro, A. C., Maia e Silva, A., Santa-Marta, M., Pombo, A., Moniz-Pereira, J., Goncalves, J., Barahona, I. (2005). Functional Analysis of Vif Protein Shows Less Restriction of Human Immunodeficiency Virus Type 2 by APOBEC3G. J. Virol. 79: 823-833 [Abstract] [Full Text]  
  • Luo, K., Liu, B., Xiao, Z., Yu, Y., Yu, X., Gorelick, R., Yu, X.-F. (2004). Amino-Terminal Region of the Human Immunodeficiency Virus Type 1 Nucleocapsid Is Required for Human APOBEC3G Packaging. J. Virol. 78: 11841-11852 [Abstract] [Full Text]  
  • Bennett, E. M., Lever, A. M. L., Allen, J. F. (2004). Human Immunodeficiency Virus Type 2 Gag Interacts Specifically with PRP4, a Serine-Threonine Kinase, and Inhibits Phosphorylation of Splicing Factor SF2. J. Virol. 78: 11303-11312 [Abstract] [Full Text]  
  • Feng, F., Davis, A., Lake, J.-A., Carr, J., Xia, W., Burrell, C., Li, P. (2004). Ring Finger Protein ZIN Interacts with Human Immunodeficiency Virus Type 1 Vif. J. Virol. 78: 10574-10581 [Abstract] [Full Text]  
  • Cen, S., Guo, F., Niu, M., Saadatmand, J., Deflassieux, J., Kleiman, L. (2004). The Interaction between HIV-1 Gag and APOBEC3G. J. Biol. Chem. 279: 33177-33184 [Abstract] [Full Text]  
  • Halwani, R., Cen, S., Javanbakht, H., Saadatmand, J., Kim, S., Shiba, K., Kleiman, L. (2004). Cellular Distribution of Lysyl-tRNA Synthetase and Its Interaction with Gag during Human Immunodeficiency Virus Type 1 Assembly. J. Virol. 78: 7553-7564 [Abstract] [Full Text]  
  • Dooher, J. E., Lingappa, J. R. (2004). Conservation of a Stepwise, Energy-Sensitive Pathway Involving HP68 for Assembly of Primate Lentivirus Capsids in Cells. J. Virol. 78: 1645-1656 [Abstract] [Full Text]  
  • Liu, B., Yu, X., Luo, K., Yu, Y., Yu, X.-F. (2004). Influence of Primate Lentiviral Vif and Proteasome Inhibitors on Human Immunodeficiency Virus Type 1 Virion Packaging of APOBEC3G. J. Virol. 78: 2072-2081 [Abstract] [Full Text]  
  • Gaddis, N. C., Chertova, E., Sheehy, A. M., Henderson, L. E., Malim, M. H. (2003). Comprehensive Investigation of the Molecular Defect in vif-Deficient Human Immunodeficiency Virus Type 1 Virions. J. Virol. 77: 5810-5820 [Abstract] [Full Text]  
  • Halwani, R., Khorchid, A., Cen, S., Kleiman, L. (2003). Rapid Localization of Gag/GagPol Complexes to Detergent-Resistant Membrane during the Assembly of Human Immunodeficiency Virus Type 1. J. Virol. 77: 3973-3984 [Abstract] [Full Text]  
  • Kao, S., Akari, H., Khan, M. A., Dettenhofer, M., Yu, X.-F., Strebel, K. (2002). Human Immunodeficiency Virus Type 1 Vif Is Efficiently Packaged into Virions during Productive but Not Chronic Infection. J. Virol. 77: 1131-1140 [Abstract] [Full Text]  
  • Madani, N., Millette, R., Platt, E. J., Marin, M., Kozak, S. L., Bloch, D. B., Kabat, D. (2002). Implication of the Lymphocyte-Specific Nuclear Body Protein Sp140 in an Innate Response to Human Immunodeficiency Virus Type 1. J. Virol. 76: 11133-11138 [Abstract] [Full Text]  
  • Goncalves, J., Silva, F., Freitas-Vieira, A., Santa-Marta, M., Malho, R., Yang, X., Gabuzda, D., Barbas, C. III (2002). Functional Neutralization of HIV-1 Vif Protein by Intracellular Immunization Inhibits Reverse Transcription and Viral Replication. J. Biol. Chem. 277: 32036-32045 [Abstract] [Full Text]  
  • Khorchid, A., Halwani, R., Wainberg, M. A., Kleiman, L. (2002). Role of RNA in Facilitating Gag/Gag-Pol Interaction. J. Virol. 76: 4131-4137 [Abstract] [Full Text]  
  • Bardy, M., Gay, B., Pebernard, S., Chazal, N., Courcoul, M., Vigne, R., Decroly, E., Boulanger, P. (2001). Interaction of human immunodeficiency virus type 1 Vif with Gag and Gag-Pol precursors: co-encapsidation and interference with viral protease-mediated Gag processing. J. Gen. Virol. 82: 2719-2733 [Abstract] [Full Text]  
  • Mouland, A. J., Xu, H., Cui, H., Krueger, W., Munro, T. P., Prasol, M., Mercier, J., Rekosh, D., Smith, R., Barbarese, E., Cohen, E. A., Carson, J. H. (2001). RNA Trafficking Signals in Human Immunodeficiency Virus Type 1. Mol. Cell. Biol. 21: 2133-2143 [Abstract] [Full Text]  
  • Henzler, T., Harmache, A., Herrmann, H., Spring, H., Suzan, M., Audoly, G., Panek, T., Bosch, V. (2001). Fully functional, naturally occurring and C-terminally truncated variant human immunodeficiency virus (HIV) Vif does not bind to HIV Gag but influences intermediate filament structure. J. Gen. Virol. 82: 561-573 [Abstract] [Full Text]  
  • Öhagen, A., Gabuzda, D. (2000). Role of Vif in Stability of the Human Immunodeficiency Virus Type 1 Core. J. Virol. 74: 11055-11066 [Abstract] [Full Text]  
  • Rousso, I., Mixon, M. B., Chen, B. K., Kim, P. S. (2000). Palmitoylation of the HIV-1 envelope glycoprotein is critical for viral infectivity. Proc. Natl. Acad. Sci. USA 10.1073/pnas.240459697v1 [Abstract] [Full Text]  
  • Dettenhofer, M., Cen, S., Carlson, B. A., Kleiman, L., Yu, X.-F. (2000). Association of Human Immunodeficiency Virus Type 1 Vif with RNA and Its Role in Reverse Transcription. J. Virol. 74: 8938-8945 [Abstract] [Full Text]  
  • Zhang, H., Pomerantz, R. J., Dornadula, G., Sun, Y. (2000). Human Immunodeficiency Virus Type 1 Vif Protein Is an Integral Component of an mRNP Complex of Viral RNA and Could Be Involved in the Viral RNA Folding and Packaging Process. J. Virol. 74: 8252-8261 [Abstract] [Full Text]  
  • Tritel, M., Resh, M. D. (2000). Kinetic Analysis of Human Immunodeficiency Virus Type 1 Assembly Reveals the Presence of Sequential Intermediates. J. Virol. 74: 5845-5855 [Abstract] [Full Text]  
  • Simon, J. H. M., Sheehy, A. M., Carpenter, E. A., Fouchier, R. A. M., Malim, M. H. (1999). Mutational Analysis of the Human Immunodeficiency Virus Type 1 Vif Protein. J. Virol. 73: 2675-2681 [Abstract] [Full Text]  
  • Yang, S., Sun, Y., Zhang, H. (2001). The Multimerization of Human Immunodeficiency Virus Type I Vif Protein. A REQUIREMENT FOR Vif FUNCTION IN THE VIRAL LIFE CYCLE. J. Biol. Chem. 276: 4889-4893 [Abstract] [Full Text]  
  • Rousso, I., Mixon, M. B., Chen, B. K., Kim, P. S. (2000). Palmitoylation of the HIV-1 envelope glycoprotein is critical for viral infectivity. Proc. Natl. Acad. Sci. USA 97: 13523-13525 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»