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Journal of Virology, March 1999, p. 2288-2297, Vol. 73, No. 3
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Tomato Spotted Wilt Virus Particle Morphogenesis in Plant Cells

Marjolein Kikkert, Jan Van Lent, Marc Storms, Pentcho Bodegom, Richard Kormelink, and Rob Goldbach*

Laboratory of Virology, Wageningen Agricultural University, Wageningen, The Netherlands

Received 11 May 1998/Accepted 30 November 1998

A model for the maturation of tomato spotted wilt virus (TSWV) particles is proposed, mainly based on results with a protoplast infection system, in which the chronology of different maturation events could be determined. By using specific monoclonal and polyclonal antisera in immunofluorescence and electron microscopy, the site of TSWV particle morphogenesis was determined to be the Golgi system. The viral glycoproteins G1 and G2 accumulate in the Golgi prior to a process of wrapping, by which the viral nucleocapsids obtain a double membrane. In a later stage of the maturation, these doubly enveloped particles fuse to each other and to the endoplasmic reticulum to form singly enveloped particles clustered in membranes. Similarities and differences between the maturation of animal-infecting (bunya)viruses and plant-infecting tospoviruses are discussed.

* Corresponding author. Mailing address: Laboratory of Virology, WAU, Binnenhaven 11, 6709 PD Wageningen, The Netherlands. Phone: 31-317-483090. Fax: 31-317-484820. E-mail: Rob.Goldbach{at}medew.viro.wau.nl.

Journal of Virology, March 1999, p. 2288-2297, Vol. 73, No. 3
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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