This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gómez del Moral, M.
Right arrow Articles by Domínguez, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gómez del Moral, M.
Right arrow Articles by Domínguez, J.

 Previous Article  |  Next Article 

Journal of Virology, March 1999, p. 2173-2180, Vol. 73, No. 3
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

African Swine Fever Virus Infection Induces Tumor Necrosis Factor Alpha Production: Implications in Pathogenesis

M. Gómez del Moral,1 E. Ortuño,1 P. Fernández-Zapatero,2 F. Alonso,1 C. Alonso,2 A. Ezquerra,1,* and J. Domínguez1

Departamento de Sanidad Animal, Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid,1 and Departamento de Biotecnología y Mejora Genética, INIA, 28040 Madrid,2 Spain

Received 30 July 1998/Accepted 2 December 1998

We have analyzed the production of tumor necrosis factor alpha (TNF-alpha ) induced by in vitro infection with African swine fever (ASF) virus (ASFV) and the systemic and local release of this inflammatory cytokine upon in vivo infection. An early increase in TNF-alpha mRNA expression was detected in ASFV-infected alveolar macrophages, and high levels of TNF-alpha protein were detected by ELISA in culture supernatants from these cells. When animals were experimentally infected with a virulent isolate (E-75), enhanced TNF-alpha expression in mainly affected organs correlated with viral protein expression. Finally, elevated levels of TNF-alpha were detected in serum, corresponding to the onset of clinical signs. TNF-alpha has been reported to be critically involved in the pathogenesis of major clinical events in ASF, such as intravascular coagulation, tissue injury, apoptosis, and shock. In the present study, TNF-alpha containing supernatants from ASFV-infected cultures induced apoptosis in uninfected lymphocytes; this effect was partially abrogated by preincubation with an anti-TNF-alpha specific antibody. These results suggest a relevant role for TNF-alpha in the pathogenesis of ASF.

* Corresponding author. Mailing address: Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, 28130 Madrid, Spain. Phone: 34-91-6202300. Fax: 34-91-6202247. E-mail: ezquerr{at}inia.es.

Journal of Virology, March 1999, p. 2173-2180, Vol. 73, No. 3
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pedrera, M., Gomez-Villamandos, J. C., Romero-Trevejo, J. L., Risalde, M. A., Molina, V., Sanchez-Cordon, P. J. (2009). Apoptosis in lymphoid tissues of calves inoculated with non-cytopathic bovine viral diarrhea virus genotype 1: activation of effector caspase-3 and role of macrophages. J. Gen. Virol. 90: 2650-2659 [Abstract] [Full Text]  
  • Zhang, F., Hopwood, P., Abrams, C. C., Downing, A., Murray, F., Talbot, R., Archibald, A., Lowden, S., Dixon, L. K. (2006). Macrophage Transcriptional Responses following In Vitro Infection with a Highly Virulent African Swine Fever Virus Isolate.. J. Virol. 80: 10514-10521 [Abstract] [Full Text]  
  • Granja, A. G., Nogal, M. L., Hurtado, C., del Aguila, C., Carrascosa, A. L., Salas, M. L., Fresno, M., Revilla, Y. (2006). The Viral Protein A238L Inhibits TNF-{alpha} Expression through a CBP/p300 Transcriptional Coactivators Pathway. J. Immunol. 176: 451-462 [Abstract] [Full Text]  
  • Sanchez-Cordon, P. J., Nunez, A., Salguero, F. J., Pedrera, M., Fernandez de Marco, M., Gomez-Villamandos, J. C. (2005). Lymphocyte Apoptosis and Thrombocytopenia in Spleen during Classical Swine Fever: Role of Macrophages and Cytokines. Vet Pathol 42: 477-488 [Abstract] [Full Text]  
  • Sanchez-Cordon, P. J., Romanini, S., Salguero, F. J., Ruiz-Villamor, E., Carrasco, L., Gomez-Villamandos, J. C. (2003). A Histopathologic, Immunohistochemical, and Ultrastructural Study of the Intestine in Pigs Inoculated with Classical Swine Fever Virus. Vet Pathol 40: 254-262 [Abstract] [Full Text]  
  • Nakamoto, Y., Kaneko, S., Kobayashi, K. (2002). Increased susceptibility to apoptosis and attenuated Bcl-2 expression in T lymphocytes and monocytes from patients with advanced chronic hepatitis C. J. Leukoc. Biol. 72: 49-55 [Abstract] [Full Text]  
  • Vallee, I., Tait, S. W. G., Powell, P. P. (2001). African Swine Fever Virus Infection of Porcine Aortic Endothelial Cells Leads to Inhibition of Inflammatory Responses, Activation of the Thrombotic State, and Apoptosis. J. Virol. 75: 10372-10382 [Abstract] [Full Text]  
  • Perkins, L. E. L., Swayne, D. E. (2001). Pathobiology of A/Chicken/Hong Kong/220/97 (H5N1) Avian Influenza Virus in Seven Gallinaceous Species. Vet Pathol 38: 149-164 [Abstract] [Full Text]  
  • Herbein, G., O'brien, W. A. (2000). Tumor Necrosis Factor (TNF)-{alpha} and TNF Receptors in Viral Pathogenesis. Exp. Biol. Med. 223: 241-257 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»