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Journal of Virology, March 1999, p. 2006-2015, Vol. 73, No. 3
Department of Developmental and Molecular
Biology, Albert Einstein College of Medicine, Bronx, New York 10461
Received 23 July 1998/Accepted 7 December 1998
During herpes simplex virus (HSV) assembly, immature procapsids
must expel their internal scaffold proteins, transform their outer
shell to form mature polyhedrons, and become packaged with the viral
double-stranded (ds) DNA genome. A large number of virally encoded
proteins are required for successful completion of these events, but
their molecular roles are poorly understood. By analogy with the dsDNA
bacteriophage we reasoned that HSV DNA packaging might be an
ATP-requiring process and tested this hypothesis by adding an ATP
depletion cocktail to cells accumulating unpackaged procapsids due to
the presence of a temperature-sensitive lesion in the HSV maturational
protease UL26. Following return to permissive temperature, HSV capsids
were found to be unable to package DNA, suggesting that this process is
indeed ATP dependent. Surprisingly, however, the display of epitopes
indicative of capsid maturation was also inhibited. We conclude that
either formation of these epitopes directly requires ATP or capsid
maturation is normally arrested by a proofreading mechanism until DNA
packaging has been successfully completed.
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
ATP Depletion Blocks Herpes Simplex Virus DNA
Packaging and Capsid Maturation
*
Corresponding author. Mailing address: Department of
Developmental and Molecular Biology, Albert Einstein College of
Medicine, 1300 Morris Park Ave., Bronx, NY 10461. Phone: (718)
430-2305. Fax: (718) 430-8567. E-mail:
wilson{at}aecom.yu.edu.
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