This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gromeier, M.
Right arrow Articles by Wimmer, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gromeier, M.
Right arrow Articles by Wimmer, E.

 Previous Article  |  Next Article 

Journal of Virology, February 1999, p. 958-964, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Dual Stem Loops within the Poliovirus Internal Ribosomal Entry Site Control Neurovirulence

Matthias Gromeier,1,* Birgit Bossert,1 Mineo Arita,2 Akio Nomoto,2 and Eckard Wimmer1

Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook, Stony Brook, New York 11794,1 and Department of Microbiology, Institute of Medical Science, University of Tokyo, Tokyo, Japan2

Received 20 July 1998/Accepted 2 November 1998

In the human central nervous system, susceptibility to poliovirus (PV) infection is largely confined to a specific subpopulation of neuronal cells. PV tropism is likely to be determined by cell-external components such as the PV receptor CD155, as well as cell-internal constraints such as the availability of a suitable microenvironment for virus propagation. We reported previously that the exchange of the cognate internal ribosomal entry site (IRES) within the 5' nontranslated region of PV with its counterpart from human rhinovirus type 2 (HRV2) can eliminate the neuropathogenic phenotype in a transgenic mouse model for poliomyelitis without diminishing the growth properties in HeLa cells. We now show that attenuation of neurovirulence of PV/HRV2 chimeras is not confined to CD155 transgenic mice but is evident also after intraspinal inoculation into Cynomolgus monkeys. We have dissected the PV and HRV2 IRES elements to determine those structures responsible for neurovirulence (or attenuation) of these chimeric viruses. We report that two adjacent stem loop structures within the IRES cooperatively determine neuropathogenicity.

* Corresponding author. Mailing address: Dept. of Molecular Genetics and Microbiology, State University of New York at Stony Brook, Stony Brook, NY 11794. Phone: (516) 632-8806. Fax: (516) 632-8891. E-mail: gromeier{at}asterix.bio.sunysb.edu.

Journal of Virology, February 1999, p. 958-964, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • de Breyne, S., Yu, Y., Unbehaun, A., Pestova, T. V., Hellen, C. U. T. (2009). Direct functional interaction of initiation factor eIF4G with type 1 internal ribosomal entry sites. Proc. Natl. Acad. Sci. USA 106: 9197-9202 [Abstract] [Full Text]  
  • Jiang, Y., Langley, B., Lubin, F. D., Renthal, W., Wood, M. A., Yasui, D. H., Kumar, A., Nestler, E. J., Akbarian, S., Beckel-Mitchener, A. C. (2008). Epigenetics in the Nervous System. J. Neurosci. 28: 11753-11759 [Abstract] [Full Text]  
  • Arita, M., Nagata, N., Iwata, N., Ami, Y., Suzaki, Y., Mizuta, K., Iwasaki, T., Sata, T., Wakita, T., Shimizu, H. (2007). An Attenuated Strain of Enterovirus 71 Belonging to Genotype A Showed a Broad Spectrum of Antigenicity with Attenuated Neurovirulence in Cynomolgus Monkeys. J. Virol. 81: 9386-9395 [Abstract] [Full Text]  
  • de Sessions, P. F., Dobrikova, E., Gromeier, M. (2007). Genetic Adaptation to Untranslated Region-Mediated Enterovirus Growth Deficits by Mutations in the Nonstructural Proteins 3AB and 3CD. J. Virol. 81: 8396-8405 [Abstract] [Full Text]  
  • Toyoda, H., Yin, J., Mueller, S., Wimmer, E., Cello, J. (2007). Oncolytic Treatment and Cure of Neuroblastoma by a Novel Attenuated Poliovirus in a Novel Poliovirus-Susceptible Animal Model. Cancer Res. 67: 2857-2864 [Abstract] [Full Text]  
  • Campbell, S. A., Mulvey, M., Mohr, I., Gromeier, M. (2007). Attenuation of Herpes Simplex Virus Neurovirulence with Picornavirus cis-Acting Genetic Elements. J. Virol. 81: 791-799 [Abstract] [Full Text]  
  • Macadam, A. J., Ferguson, G., Stone, D. M., Meredith, J., Knowlson, S., Auda, G., Almond, J. W., Minor, P. D. (2006). Rational design of genetically stable, live-attenuated poliovirus vaccines of all three serotypes: relevance to poliomyelitis eradication.. J. Virol. 80: 8653-8663 [Abstract] [Full Text]  
  • Merrill, M. K., Gromeier, M. (2006). The Double-Stranded RNA Binding Protein 76:NF45 Heterodimer Inhibits Translation Initiation at the Rhinovirus Type 2 Internal Ribosome Entry Site. J. Virol. 80: 6936-6942 [Abstract] [Full Text]  
  • Merrill, M. K., Dobrikova, E. Y., Gromeier, M. (2006). Cell-Type-Specific Repression of Internal Ribosome Entry Site Activity by Double-Stranded RNA-Binding Protein 76.. J. Virol. 80: 3147-3156 [Abstract] [Full Text]  
  • Kauder, S., Kan, S., Racaniello, V. R. (2006). Age-Dependent Poliovirus Replication in the Mouse Central Nervous System Is Determined by Internal Ribosome Entry Site-Mediated Translation. J. Virol. 80: 2589-2595 [Abstract] [Full Text]  
  • DeJesus, N., Franco, D., Paul, A., Wimmer, E., Cello, J. (2005). Mutation of a Single Conserved Nucleotide between the Cloverleaf and Internal Ribosome Entry Site Attenuates Poliovirus Neurovirulence. J. Virol. 79: 14235-14243 [Abstract] [Full Text]  
  • Rattiner, L. M., Davis, M., Ressler, K. J. (2005). Brain-Derived Neurotrophic Factor in Amygdala-Dependent Learning. Neuroscientist 11: 323-333 [Abstract]  
  • Harvala, H., Kalimo, H., Bergelson, J., Stanway, G., Hyypia, T. (2005). Tissue tropism of recombinant coxsackieviruses in an adult mouse model. J. Gen. Virol. 86: 1897-1907 [Abstract] [Full Text]  
  • Campbell, S. A., Lin, J., Dobrikova, E. Y., Gromeier, M. (2005). Genetic Determinants of Cell Type-Specific Poliovirus Propagation in HEK 293 Cells. J. Virol. 79: 6281-6290 [Abstract] [Full Text]  
  • Wahid, R., Cannon, M. J., Chow, M. (2005). Dendritic Cells and Macrophages Are Productively Infected by Poliovirus. J. Virol. 79: 401-409 [Abstract] [Full Text]  
  • Rattiner, L. M., Davis, M., Ressler, K. J. (2004). Differential regulation of brain-derived neurotrophic factor transcripts during the consolidation of fear learning. Learn. Mem. 11: 727-731 [Abstract] [Full Text]  
  • Merrill, M. K., Bernhardt, G., Sampson, J. H., Wikstrand, C. J., Bigner, D. D., Gromeier, M. (2004). Poliovirus receptor CD155-targeted oncolysis of glioma. Neuro Oncol Duke 6: 208-217 [Abstract]  
  • Dobrikova, E., Florez, P., Bradrick, S., Gromeier, M. (2003). Activity of a type 1 picornavirus internal ribosomal entry site is determined by sequences within the 3' nontranslated region. Proc. Natl. Acad. Sci. USA 100: 15125-15130 [Abstract] [Full Text]  
  • Yanagiya, A., Ohka, S., Hashida, N., Okamura, M., Taya, C., Kamoshita, N., Iwasaki, K., Sasaki, Y., Yonekawa, H., Nomoto, A. (2003). Tissue-Specific Replicating Capacity of a Chimeric Poliovirus That Carries the Internal Ribosome Entry Site of Hepatitis C Virus in a New Mouse Model Transgenic for the Human Poliovirus Receptor. J. Virol. 77: 10479-10487 [Abstract] [Full Text]  
  • Ochs, K., Zeller, A., Saleh, L., Bassili, G., Song, Y., Sonntag, A., Niepmann, M. (2002). Impaired Binding of Standard Initiation Factors Mediates Poliovirus Translation Attenuation. J. Virol. 77: 115-122 [Abstract] [Full Text]  
  • Harvala, H., Kalimo, H., Dahllund, L., Santti, J., Hughes, P., Hyypia, T., Stanway, G. (2002). Mapping of tissue tropism determinants in coxsackievirus genomes. J. Gen. Virol. 83: 1697-1706 [Abstract] [Full Text]  
  • Ochs, K., Saleh, L., Bassili, G., Sonntag, V. H., Zeller, A., Niepmann, M. (2002). Interaction of Translation Initiation Factor eIF4B with the Poliovirus Internal Ribosome Entry Site. J. Virol. 76: 2113-2122 [Abstract] [Full Text]  
  • Bradrick, S. S., Lieben, E. A., Carden, B. M., Romero, J. R. (2001). A Predicted Secondary Structural Domain within the Internal Ribosome Entry Site of Echovirus 12 Mediates a Cell-Type-Specific Block to Viral Replication. J. Virol. 75: 6472-6481 [Abstract] [Full Text]  
  • Zhao, W. D., Lahser, F. C., Wimmer, E. (2000). Genetic Analysis of a Poliovirus/Hepatitis C Virus (HCV) Chimera: Interaction between the Poliovirus Cloverleaf and a Sequence in the HCV 5' Nontranslated Region Results in a Replication Phenotype. J. Virol. 74: 6223-6226 [Abstract] [Full Text]  
  • Gale, M. Jr., Tan, S.-L., Katze, M. G. (2000). Translational Control of Viral Gene Expression in Eukaryotes. Microbiol. Mol. Biol. Rev. 64: 239-280 [Abstract] [Full Text]  
  • Schneider-Schaulies, J. (2000). Cellular receptors for viruses: links to tropism and pathogenesis. J. Gen. Virol. 81: 1413-1429 [Full Text]  
  • Dunn, J. J., Chapman, N. M., Tracy, S., Romero, J. R. (2000). Genomic Determinants of Cardiovirulence in Coxsackievirus B3 Clinical Isolates: Localization to the 5' Nontranslated Region. J. Virol. 74: 4787-4794 [Abstract] [Full Text]  
  • Crotty, S., Lohman, B. L., Lu, F. X.-S., Tang, S., Miller, C. J., Andino, R. (1999). Mucosal Immunization of Cynomolgus Macaques with Two Serotypes of Live Poliovirus Vectors Expressing Simian Immunodeficiency Virus Antigens: Stimulation of Humoral, Mucosal, and Cellular Immunity. J. Virol. 73: 9485-9495 [Abstract] [Full Text]  
  • Kew, O., Morris-Glasgow, V., Landaverde, M., Burns, C., Shaw, J., Garib, Z., Andre, J., Blackman, E., Freeman, C. J., Jorba, J., Sutter, R., Tambini, G., Venczel, L., Pedreira, C., Laender, F., Shimizu, H., Yoneyama, T., Miyamura, T., van der Avoort, H., Oberste, M. S., Kilpatrick, D., Cochi, S., Pallansch, M., de Quadros, C. (2002). Outbreak of Poliomyelitis in Hispaniola Associated with Circulating Type 1 Vaccine-Derived Poliovirus. Science 296: 356-359 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»