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Journal of Virology, February 1999, p. 1630-1636, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Expression of Epstein-Barr Virus Nuclear Antigen 1 Is Associated with Enhanced Expression of CD25 in the Hodgkin Cell Line L428

D. Kube,1,2,* M. Vockerodt,1,2 O. Weber,3 K. Hell,4 J. Wolf,1 B. Haier,1 F. A. Grässer,5 N. Müller-Lantzsch,5 E. Kieff,6 V. Diehl,1 and H. Tesch1

Klinik für Innere Medizin I,1 Zentrum für Molekulare Medizin der Universität Köln,2 and Institut für Pathologie der Universität Köln,4 Cologne, Institut für Virologie der Universität Homburg, Homburg,5 and Bayer AG, Wuppertal,3 Germany, and Department of Medicine and Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts6

Received 30 June 1998/Accepted 20 October 1998

Epstein-Barr virus is associated with several human malignancies including Burkitt's lymphoma, nasopharyngeal carcinoma, and Hodgkin's disease (HD). To examine the effect of Epstein-Barr virus nuclear antigen 1 (EBNA-1) in the pathogenesis of HD, we transfected the gene into the HD cell line L428. EBNA-1 expression was associated with significantly enhanced CD25 expression (interleukin 2 [IL-2]-receptor alpha  chain) in transient and stably transfected L428 cells but did not affect the expression of IL-2 receptor beta  and gamma  chains. There was no up-regulation of the B-cell activation molecules CD23, CD30, CD39, CD40, CD44, CD71, and CD54 (intercellular adhesion molecule 1) or enhanced production of IL-6, IL-10, lymphotoxin alpha, and the soluble form of CD25. Stable EBNA-1-expressing L428 cells were nontumorigenic in SCID mice but showed enhanced lymphoma development in nonobese diabetic-SCID mice compared to mock-transfected cells.

* Corresponding author. Present address: Universität Tübingen, Institut für Tropenmedizin Sektion Humanparasitologie, Wilhelmstrasse 27, D-72074 Tübingen, Germany. Phone: 49 7071 2980 240. Fax: 49 7071 295189. E-mail: ail14{at}pop.rrz.uni-koeln.de.

Journal of Virology, February 1999, p. 1630-1636, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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