This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Michou, A.-I.
Right arrow Articles by Cotten, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Michou, A.-I.
Right arrow Articles by Cotten, M.

 Previous Article  |  Next Article 

Journal of Virology, February 1999, p. 1399-1410, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Mutational Analysis of the Avian Adenovirus CELO, Which Provides a Basis for Gene Delivery Vectors

Anne-Isabelle Michou, Heike Lehrmann, Mediyha Saltik, and Matt Cotten*

Institute for Molecular Pathology, 1030 Vienna, Austria

Received 23 July 1998/Accepted 20 October 1998

The avian adenovirus CELO is being developed as a gene transfer tool. Using homologous recombination in Escherichia coli, the CELO genome was screened for regions that could be deleted and would tolerate the insertion of a marker gene (luciferase or enhanced green fluorescent protein). For each mutant genome, the production of viable virus able to deliver the transgene to target cells was monitored. A series of mutants in the genome identified a set of open reading frames that could be deleted but which must be supplied in trans for virus replication. A region of the genome which is dispensable for viral replication and allows the insertion of an expression cassette was identified and a vector based on this mutation was evaluated as a gene delivery reagent. Transduction of avian cells occurs at 10- to 100-fold greater efficiency (per virus particle) than with an adenovirus type 5 (Ad5)-based vector carrying the same expression cassette. Most important for gene transfer applications, the CELO vector transduced mammalian cells as efficiently as an Ad5 vector. The CELO vector is exceptionally stable, can be grown inexpensively in chicken embryos, and provides a useful alternative to Ad5-based vectors.

* Corresponding author. Mailing address: Institute for Molecular Pathology, Dr. Bohr Gasse 7, 1030 Vienna, Austria. Phone: 43 1 797 30 526. Fax: 43 1 798 71 53. E-mail: Cotten{at}nt.imp.univie.ac.at.

Journal of Virology, February 1999, p. 1399-1410, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jaggi, F., Cabrita, M. A., Perl, A.-K. T., Christofori, G. (2008). Modulation of Endocrine Pancreas Development but not {beta}-Cell Carcinogenesis by Sprouty4. Mol Cancer Res 6: 468-482 [Abstract] [Full Text]  
  • Logunov, D. Y., Zubkova, O. V., Karyagina-Zhulina, A. S., Shuvalova, E. A., Karpov, A. P., Shmarov, M. M., Tutykhina, I. L., Alyapkina, Y. S., Grezina, N. M., Zinovieva, N. A., Ernst, L. K., Gintsburg, A. L., Naroditsky, B. S. (2007). Identification of HI-Like Loop in CELO Adenovirus Fiber for Incorporation of Receptor Binding Motifs. J. Virol. 81: 9641-9652 [Abstract] [Full Text]  
  • Cabrita, M. A., Jaggi, F., Widjaja, S. P., Christofori, G. (2006). A Functional Interaction between Sprouty Proteins and Caveolin-1. J. Biol. Chem. 281: 29201-2912 [Abstract] [Full Text]  
  • Le Goff, F., Mederle-Mangeot, I., Jestin, A., Langlois, P. (2005). Deletion of open reading frames 9, 10 and 11 from the avian adenovirus CELO genome: effect on biodistribution and humoral responses. J. Gen. Virol. 86: 2019-2027 [Abstract] [Full Text]  
  • Eickhoff, J. E., Cotten, M. (2005). NF-{kappa}B activation can mediate inhibition of human cytomegalovirus replication. J. Gen. Virol. 86: 285-295 [Abstract] [Full Text]  
  • Vadivelu, S. K., Kurzbauer, R., Dieplinger, B., Zweyer, M., Schafer, R., Wernig, A., Vietor, I., Huber, L. A. (2004). Muscle Regeneration and Myogenic Differentiation Defects in Mice Lacking TIS7. Mol. Cell. Biol. 24: 3514-3525 [Abstract] [Full Text]  
  • Eickhoff, J., Hanke, M., Stein-Gerlach, M., Kiang, T. P., Herzberger, K., Habenberger, P., Muller, S., Klebl, B., Marschall, M., Stamminger, T., Cotten, M. (2004). RICK Activates a NF-{kappa}B-dependent Anti-human Cytomegalovirus Response. J. Biol. Chem. 279: 9642-9652 [Abstract] [Full Text]  
  • Ursu, K., Harrach, B., Matiz, K., Benko, M. (2004). DNA sequencing and analysis of the right-hand part of the genome of the unique bovine adenovirus type 10. J. Gen. Virol. 85: 593-601 [Abstract] [Full Text]  
  • Cotten, M., Stegmueller, K., Eickhoff, J., Hanke, M., Herzberger, K., Herget, T., Choidas, A., Daub, H., Godl, K. (2003). Exploiting features of adenovirus replication to support mammalian kinase production. Nucleic Acids Res 31: e128-e128 [Abstract] [Full Text]  
  • Kumin, D., Hofmann, C., Rudolph, M., Both, G. W., Loser, P. (2002). Biology of Ovine Adenovirus Infection of Nonpermissive Cells. J. Virol. 76: 10882-10893 [Abstract] [Full Text]  
  • Daub, H., Blencke, S., Habenberger, P., Kurtenbach, A., Dennenmoser, J., Wissing, J., Ullrich, A., Cotten, M. (2002). Identification of SRPK1 and SRPK2 as the Major Cellular Protein Kinases Phosphorylating Hepatitis B Virus Core Protein. J. Virol. 76: 8124-8137 [Abstract] [Full Text]  
  • Tan, P. K., Michou, A.-I., Bergelson, J. M., Cotten, M. (2001). Defining CAR as a cellular receptor for the avian adenovirus CELO using a genetic analysis of the two viral fibre proteins. J. Gen. Virol. 82: 1465-1472 [Abstract] [Full Text]  
  • François, A., Eterradossi, N., Delmas, B., Payet, V., Langlois, P. (2001). Construction of Avian Adenovirus CELO Recombinants in Cosmids. J. Virol. 75: 5288-5301 [Abstract] [Full Text]  
  • Impagnatiello, M.-A., Weitzer, S., Gannon, G., Compagni, A., Cotten, M., Christofori, G. (2001). Mammalian Sprouty-1 and -2 Are Membrane-Anchored Phosphoprotein Inhibitors of Growth Factor Signaling in Endothelial Cells. JCB 152: 1087-1098 [Abstract] [Full Text]  
  • Glotzer, J. B., Michou, A.-I., Baker, A., Saltik, M., Cotten, M. (2001). Microtubule-Independent Motility and Nuclear Targeting of Adenoviruses with Fluorescently Labeled Genomes. J. Virol. 75: 2421-2434 [Abstract] [Full Text]  
  • Compagni, A., Wilgenbus, P., Impagnatiello, M.-A., Cotten, M., Christofori, G. (2000). Fibroblast Growth Factors Are Required for Efficient Tumor Angiogenesis. Cancer Res. 60: 7163-7169 [Abstract] [Full Text]  
  • Soudais, C., Boutin, S., Hong, S. S., Chillon, M., Danos, O., Bergelson, J. M., Boulanger, P., Kremer, E. J. (2000). Canine Adenovirus Type 2 Attachment and Internalization: Coxsackievirus-Adenovirus Receptor, Alternative Receptors, and an RGD-Independent Pathway. J. Virol. 74: 10639-10649 [Abstract] [Full Text]  
  • Russell, W. C. (2000). Update on adenovirus and its vectors. J. Gen. Virol. 81: 2573-2604 [Full Text]  
  • Kremer, E. J., Boutin, S., Chillon, M., Danos, O. (2000). Canine Adenovirus Vectors: an Alternative for Adenovirus-Mediated Gene Transfer. J. Virol. 74: 505-512 [Abstract] [Full Text]  
  • Lehrmann, H., Cotten, M. (1999). Characterization of CELO Virus Proteins That Modulate the pRb/E2F Pathway. J. Virol. 73: 6517-6525 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»