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Journal of Virology, February 1999, p. 1350-1361, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Interactions of the Cytoplasmic Domains of Human and Simian Retroviral Transmembrane Proteins with Components of the Clathrin Adaptor Complexes Modulate Intracellular and Cell Surface Expression of Envelope Glycoproteins

Clarisse Berlioz-Torrent,1 Barbara L. Shacklett,2 Lars Erdtmann,1 Lelia Delamarre,3 Isabelle Bouchaert,4 Pierre Sonigo,2 Marie Christine Dokhelar,3 and Richard Benarous1,*

CJF 97/03 INSERM, Interactions Moléculaires, Hôte-Pathogène,1 Génétique des virus CNRS UPR 0415,2 INSERM U332,3 and Service de Cytométrie,4 Institut Cochin de Génétique Moléculaire, 75014 Paris, France

Received 13 July 1998/Accepted 30 October 1998

The cytoplasmic domains of the transmembrane (TM) envelope proteins (TM-CDs) of most retroviruses have a Tyr-based motif, YXXØ, in their membrane-proximal regions. This signal is involved in the trafficking and endocytosis of membrane receptors via clathrin-associated AP-1 and AP-2 adaptor complexes. We have used CD8-TM-CD chimeras to investigate the role of the Tyr-based motif of human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus (SIV), and human T-leukemia virus type 1 (HTLV-1) TM-CDs in the cell surface expression of the envelope glycoprotein. Flow cytometry and confocal microscopy studies showed that this motif is a major determinant of the cell surface expression of the CD8-HTLV chimera. The YXXØ motif also plays a key role in subcellular distribution of the envelope of lentiviruses HIV-1 and SIV. However, these viruses, which encode TM proteins with a long cytoplasmic domain, have additional determinants distal to the YXXØ motif that participate in regulating cell surface expression. We have also used the yeast two-hybrid system and in vitro binding assays to demonstrate that all three retroviral YXXØ motifs interact with the µ1 and µ2 subunits of AP complexes and that the C-terminal regions of HIV-1 and SIV TM proteins interact with the beta 2 adaptin subunit. The TM-CDs of HTLV-1, HIV-1, and SIV also interact with the whole AP complexes. These results clearly demonstrate that the cell surface expression of retroviral envelope glycoproteins is governed by interactions with adaptor complexes. The YXXØ-based signal is the major determinant of this interaction for the HTLV-1 TM, which contains a short cytoplasmic domain, whereas the lentiviruses HIV-1 and SIV have additional determinants distal to this signal that are also involved.

* Corresponding author. Mailing address: CJF 97/03 INSERM, Interactions Moléculaires Hôte-Pathogène, Institut Cochin de Génétique Moléculaire (ICGM), 24 rue du faubourg St Jacques, 75014 Paris, France. Phone: 33 1 44 41 24 65 Fax: 33 1 44 41 23 99. E-mail: benarous{at}icgm.cochin.inserm.fr.

Journal of Virology, February 1999, p. 1350-1361, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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