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Journal of Virology, February 1999, p. 1341-1349, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Identification and Characterization of Kaposi's
Sarcoma-Associated Herpesvirus K8.1 Virion Glycoprotein
Mengtao
Li,1
John
MacKey,2
Susan C.
Czajak,1
Ronald C.
Desrosiers,1
Andrew A.
Lackner,2 and
Jae U.
Jung1,*
Department of Microbiology and Molecular
Genetics1 and
Department of
Pathology,2 New England Regional Primate
Research Center, Harvard Medical School, Southborough, Massachusetts
01772-9102
Received 28 August 1998/Accepted 9 November 1998
Kaposi's sarcoma-associated herpesvirus (KSHV) has been
consistently identified in Kaposi's sarcomas (KS), body cavity-based lymphomas (BCBL), and some forms of Castleman's disease. Previous serological tests with KS patient sera have detected lytic-cycle polypeptides from KSHV-infected BCBL cells. We have found that these
polypeptides are predominantly encoded by the K8.1 open reading frame,
which is present in the same genomic position as virion envelope
glycoproteins of other gammaherpesviruses. The cDNA of K8.1 from BCBL-1
cells was found to encode a glycosylated protein with an apparent
molecular mass of 37 kDa. K8.1 was found to be expressed during lytic
KSHV replication in BCBL-1 cells and was localized on the surface of
cells and virions. The results of immunofluorescence and immunoelectron
microscopy suggest that KSHV acquires K8.1 protein on its virion
surface during the process of budding at the plasma cell membrane. When
KSHV K8.1 derived from mammalian cells was used as an antigen in
immunoblot tests, antibodies to K8.1 were detected in 18 of 20 KS
patients and in 0 of 10 KS-negative control subjects. These results
demonstrate that the K8.1 gene encodes a KSHV virion-associated
glycoprotein and suggest that antibodies to K8.1 may prove useful as
contributory serological markers for infection by KSHV.
*
Corresponding author. Mailing address: New England
Regional Primate Research Center, Harvard Medical School, P.O. Box
9102, Southborough, MA 01772-9102. Phone: (508) 624-8083. Fax: (508) 624-8190. E-mail: jjung{at}warren.med.harvard.edu.
Journal of Virology, February 1999, p. 1341-1349, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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