Cloning and Characterization of Adeno-Associated Virus Type 5

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Chiorini, J. A.
	Articles by Kotin, R. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Chiorini, J. A.
	Articles by Kotin, R. M.

Previous Article | Next Article

Journal of Virology, February 1999, p. 1309-1319, Vol. 73, No. 2
0022-538X/99/$00.00+0

Cloning and Characterization of Adeno-Associated Virus Type 5

John A. Chiorini, Frank Kim, Linda Yang, and Robert M. Kotin^*

Molecular Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, Maryland 20892

Received 26 August 1998/Accepted 30 October 1998

Adeno-associated virus type 5 (AAV5) is distinct from other dependovirus serotypes based on DNA hybridization and serologicaldata. To better understand the biology of AAV5, we have clonedand sequenced its genome and generated recombinant AAV5 particles.The single-stranded DNA genome is similar in length and geneticorganization to that of AAV2. The rep gene of AAV5 is 67% homologousto AAV2, with the majority of the changes occurring in the carboxyland amino termini. This homology is much less than that observedwith other reported AAV serotypes. The inverted terminal repeats(ITRs) are also unique compared to those of the other AAV serotypes.While the characteristic AAV hairpin structure and the Rep DNAbinding site are retained, the consensus terminal resolution siteis absent. These differences in the Rep proteins and the ITRsresult in a lack of cross-complementation between AAV2 and AAV5as measured by the production of recombinant AAV particles. Alignmentof the cap open reading frame with that of the other AAV serotypesidentifies both conserved and variable regions which could affecttissue tropism and particle stability. Comparison of transductionefficiencies in a variety of cells lines and a lack of inhibitionby soluble heparin indicate that AAV5 may utilize a distinct mechanismof uptake compared toAAV2.

^* Corresponding author. Mailing address: Molecular Hematology Branch, NIH, NHLBI, Bldg. 10, 7D18, 10 Center Dr. MSC 1654, Bethesda,MD 20892-1654. Phone: (301) 496-1594. Fax: (301) 496-9985. E-mail:Kotinr{at}fido.nhlbi.nih.gov.

Journal of Virology, February 1999, p. 1309-1319, Vol. 73, No. 2
0022-538X/99/$00.00+0

This article has been cited by other articles:

Schmidt, M., Govindasamy, L., Afione, S., Kaludov, N., Agbandje-McKenna, M., Chiorini, J. A. (2008). Molecular Characterization of the Heparin-Dependent Transduction Domain on the Capsid of a Novel Adeno-Associated Virus Isolate, AAV(VR-942). J. Virol. 82: 8911-8916 [Abstract] [Full Text]
Sumner-Jones, S. G., Gill, D. R., Hyde, S. C. (2007). Lack of Repeat Transduction by Recombinant Adeno-Associated Virus Type 5/5 Vectors in the Mouse Airway. J. Virol. 81: 12360-12367 [Abstract] [Full Text]
Allocca, M., Mussolino, C., Garcia-Hoyos, M., Sanges, D., Iodice, C., Petrillo, M., Vandenberghe, L. H., Wilson, J. M., Marigo, V., Surace, E. M., Auricchio, A. (2007). Novel Adeno-Associated Virus Serotypes Efficiently Transduce Murine Photoreceptors. J. Virol. 81: 11372-11380 [Abstract] [Full Text]
Wang, J., Xie, J., Lu, H., Chen, L., Hauck, B., Samulski, R. J., Xiao, W. (2007). Existence of transient functional double-stranded DNA intermediates during recombinant AAV transduction. Proc. Natl. Acad. Sci. USA 104: 13104-13109 [Abstract] [Full Text]
Ye, C., Pintel, D. J. (2007). Upstream AP1- and CREB-Binding Sites Confer High Basal Activity on the Adeno-Associated Virus Type 5 Capsid Gene Promoter. J. Virol. 81: 2605-2613 [Abstract] [Full Text]
Xin, K.-Q., Mizukami, H., Urabe, M., Toda, Y., Shinoda, K., Yoshida, A., Oomura, K., Kojima, Y., Ichino, M., Klinman, D., Ozawa, K., Okuda, K. (2006). Induction of Robust Immune Responses against Human Immunodeficiency Virus Is Supported by the Inherent Tropism of Adeno-Associated Virus Type 5 for Dendritic Cells. J. Virol. 80: 11899-11910 [Abstract] [Full Text]
Govindasamy, L., Padron, E., McKenna, R., Muzyczka, N., Kaludov, N., Chiorini, J. A., Agbandje-McKenna, M. (2006). Structurally Mapping the Diverse Phenotype of Adeno-Associated Virus Serotype 4. J. Virol. 80: 11556-11570 [Abstract] [Full Text]
Qiu, J., Cheng, F., Pintel, D. J. (2006). Expression profiles of bovine adeno-associated virus and avian adeno-associated virus display significant similarity to that of adeno-associated virus type 5.. J. Virol. 80: 5482-5493 [Abstract] [Full Text]
Nathwani, A. C., Gray, J. T., Ng, C. Y. C., Zhou, J., Spence, Y., Waddington, S. N., Tuddenham, E. G. D., Kemball-Cook, G., McIntosh, J., Boon-Spijker, M., Mertens, K., Davidoff, A. M. (2006). Self-complementary adeno-associated virus vectors containing a novel liver-specific human factor IX expression cassette enable highly efficient transduction of murine and nonhuman primate liver. Blood 107: 2653-2661 [Abstract] [Full Text]
Urabe, M., Nakakura, T., Xin, K.-Q., Obara, Y., Mizukami, H., Kume, A., Kotin, R. M., Ozawa, K. (2006). Scalable Generation of High-Titer Recombinant Adeno-Associated Virus Type 5 in Insect Cells. J. Virol. 80: 1874-1885 [Abstract] [Full Text]
Grimm, D., Pandey, K., Nakai, H., Storm, T. A., Kay, M. A. (2006). Liver Transduction with Recombinant Adeno-Associated Virus Is Primarily Restricted by Capsid Serotype Not Vector Genotype. J. Virol. 80: 426-439 [Abstract] [Full Text]
Arbetman, A. E., Lochrie, M., Zhou, S., Wellman, J., Scallan, C., Doroudchi, M. M., Randlev, B., Patarroyo-White, S., Liu, T., Smith, P., Lehmkuhl, H., Hobbs, L. A., Pierce, G. F., Colosi, P. (2005). Novel Caprine Adeno-Associated Virus (AAV) Capsid (AAV-Go.1) Is Closely Related to the Primate AAV-5 and Has Unique Tropism and Neutralization Properties. J. Virol. 79: 15238-15245 [Abstract] [Full Text]
Zhu, T., Zhou, L., Mori, S., Wang, Z., McTiernan, C. F., Qiao, C., Chen, C., Wang, D. W., Li, J., Xiao, X. (2005). Sustained Whole-Body Functional Rescue in Congestive Heart Failure and Muscular Dystrophy Hamsters by Systemic Gene Transfer. Circulation 112: 2650-2659 [Abstract] [Full Text]
Streck, C. J., Dickson, P. V., Ng, C. Y.C., Zhou, J., Gray, J. T., Nathwani, A. C., Davidoff, A. M. (2005). Adeno-Associated Virus Vector-Mediated Systemic Delivery of IFN-{beta} Combined with Low-Dose Cyclophosphamide Affects Tumor Regression in Murine Neuroblastoma Models. Clin. Cancer Res. 11: 6020-6029 [Abstract] [Full Text]
Wang, L., Calcedo, R., Nichols, T. C., Bellinger, D. A., Dillow, A., Verma, I. M., Wilson, J. M. (2005). Sustained correction of disease in naive and AAV2-pretreated hemophilia B dogs: AAV2/8-mediated, liver-directed gene therapy. Blood 105: 3079-3086 [Abstract] [Full Text]
Le Meur, G., Weber, M., Pereon, Y., Mendes-Madeira, A., Nivard, D., Deschamps, J.-Y., Moullier, P., Rolling, F. (2005). Postsurgical Assessment and Long-term Safety of Recombinant Adeno-Associated Virus-Mediated Gene Transfer Into the Retinas of Dogs and Primates. Arch Ophthalmol 123: 500-506 [Abstract] [Full Text]
Jang, M. Y., Yarborough, O. H. III, Conyers, G. B., McPhie, P., Owens, R. A. (2005). Stable Secondary Structure near the Nicking Site for Adeno-Associated Virus Type 2 Rep Proteins on Human Chromosome 19. J. Virol. 79: 3544-3556 [Abstract] [Full Text]
Ostedgaard, L. S., Rokhlina, T., Karp, P. H., Lashmit, P., Afione, S., Schmidt, M., Zabner, J., Stinski, M. F., Chiorini, J. A., Welsh, M. J. (2005). A shortened adeno-associated virus expression cassette for CFTR gene transfer to cystic fibrosis airway epithelia. Proc. Natl. Acad. Sci. USA 102: 2952-2957 [Abstract] [Full Text]
Yan, Z., Zak, R., Zhang, Y., Engelhardt, J. F. (2005). Inverted Terminal Repeat Sequences Are Important for Intermolecular Recombination and Circularization of Adeno-Associated Virus Genomes. J. Virol. 79: 364-379 [Abstract] [Full Text]
Sandalon, Z., Bruckheimer, E. M., Lustig, K. H., Rogers, L. C., Peluso, R. W., Burstein, H. (2004). Secretion of a TNFR:Fc Fusion Protein following Pulmonary Administration of Pseudotyped Adeno-Associated Virus Vectors. J. Virol. 78: 12355-12365 [Abstract] [Full Text]
Cressant, A., Desmaris, N., Verot, L., Brejot, T., Froissart, R., Vanier, M.-T., Maire, I., Heard, J. M. (2004). Improved Behavior and Neuropathology in the Mouse Model of Sanfilippo Type IIIB Disease after Adeno-Associated Virus-Mediated Gene Transfer in the Striatum. J. Neurosci. 24: 10229-10239 [Abstract] [Full Text]
Paterna, J.-C., Feldon, J., Bueler, H. (2004). Transduction Profiles of Recombinant Adeno-Associated Virus Vectors Derived from Serotypes 2 and 5 in the Nigrostriatal System of Rats. J. Virol. 78: 6808-6817 [Abstract] [Full Text]
Schmidt, M., Katano, H., Bossis, I., Chiorini, J. A. (2004). Cloning and Characterization of a Bovine Adeno-Associated Virus. J. Virol. 78: 6509-6516 [Abstract] [Full Text]
Rabinowitz, J. E., Bowles, D. E., Faust, S. M., Ledford, J. G., Cunningham, S. E., Samulski, R. J. (2004). Cross-Dressing the Virion: the Transcapsidation of Adeno-Associated Virus Serotypes Functionally Defines Subgroups. J. Virol. 78: 4421-4432 [Abstract] [Full Text]
Iwata, N., Mizukami, H., Shirotani, K., Takaki, Y., Muramatsu, S.-i., Lu, B., Gerard, N. P., Gerard, C., Ozawa, K., Saido, T. C. (2004). Presynaptic Localization of Neprilysin Contributes to Efficient Clearance of Amyloid-{beta} Peptide in Mouse Brain. J. Neurosci. 24: 991-998 [Abstract] [Full Text]
Qiu, J., Nayak, R., Pintel, D. J. (2004). Alternative Polyadenylation of Adeno-Associated Virus Type 5 RNA within an Internal Intron Is Governed by both a Downstream Element within the Intron 3' Splice Acceptor and an Element Upstream of the P41 Initiation Site. J. Virol. 78: 83-93 [Abstract] [Full Text]
Grimm, D., Zhou, S., Nakai, H., Thomas, C. E., Storm, T. A., Fuess, S., Matsushita, T., Allen, J., Surosky, R., Lochrie, M., Meuse, L., McClelland, A., Colosi, P., Kay, M. A. (2003). Preclinical in vivo evaluation of pseudotyped adeno-associated virus vectors for liver gene therapy. Blood 102: 2412-2419 [Abstract] [Full Text]
Davidoff, A. M., Ng, C. Y. C., Zhou, J., Spence, Y., Nathwani, A. C. (2003). Sex significantly influences transduction of murine liver by recombinant adeno-associated viral vectors through an androgen-dependent pathway. Blood 102: 480-488 [Abstract] [Full Text]
Bossis, I., Chiorini, J. A. (2003). Cloning of an Avian Adeno-Associated Virus (AAAV) and Generation of Recombinant AAAV Particles. J. Virol. 77: 6799-6810 [Abstract] [Full Text]
Opie, S. R., Warrington, K. H. Jr., Agbandje-McKenna, M., Zolotukhin, S., Muzyczka, N. (2003). Identification of Amino Acid Residues in the Capsid Proteins of Adeno-Associated Virus Type 2 That Contribute to Heparan Sulfate Proteoglycan Binding. J. Virol. 77: 6995-7006 [Abstract] [Full Text]
Passini, M. A., Watson, D. J., Vite, C. H., Landsburg, D. J., Feigenbaum, A. L., Wolfe, J. H. (2003). Intraventricular Brain Injection of Adeno-Associated Virus Type 1 (AAV1) in Neonatal Mice Results in Complementary Patterns of Neuronal Transduction to AAV2 and Total Long-Term Correction of Storage Lesions in the Brains of {beta}-Glucuronidase-Deficient Mice. J. Virol. 77: 7034-7040 [Abstract] [Full Text]
Gao, G., Alvira, M. R., Somanathan, S., Lu, Y., Vandenberghe, L. H., Rux, J. J., Calcedo, R., Sanmiguel, J., Abbas, Z., Wilson, J. M. (2003). Adeno-associated viruses undergo substantial evolution in primates during natural infections. Proc. Natl. Acad. Sci. USA 100: 6081-6086 [Abstract] [Full Text]
Hauck, B., Xiao, W. (2003). Characterization of Tissue Tropism Determinants of Adeno-Associated Virus Type 1. J. Virol. 77: 2768-2774 [Abstract] [Full Text]
Yoon-Robarts, M., Linden, R. M. (2003). Identification of Active Site Residues of the Adeno-associated Virus Type 2 Rep Endonuclease. J. Biol. Chem. 278: 4912-4918 [Abstract] [Full Text]
Bowles, D. E., Rabinowitz, J. E., Samulski, R. J. (2002). Marker Rescue of Adeno-Associated Virus (AAV) Capsid Mutants: a Novel Approach for Chimeric AAV Production. J. Virol. 77: 423-432 [Abstract] [Full Text]
Qiu, J., Nayak, R., Tullis, G. E., Pintel, D. J. (2002). Characterization of the Transcription Profile of Adeno-Associated Virus Type 5 Reveals a Number of Unique Features Compared to Previously Characterized Adeno-Associated Viruses. J. Virol. 76: 12435-12447 [Abstract] [Full Text]
Mastakov, M. Y., Baer, K., Symes, C. W., Leichtlein, C. B., Kotin, R. M., During, M. J. (2002). Immunological Aspects of Recombinant Adeno-Associated Virus Delivery to the Mammalian Brain. J. Virol. 76: 8446-8454 [Abstract] [Full Text]
Yang, G. S., Schmidt, M., Yan, Z., Lindbloom, J. D., Harding, T. C., Donahue, B. A., Engelhardt, J. F., Kotin, R., Davidson, B. L. (2002). Virus-Mediated Transduction of Murine Retina with Adeno-Associated Virus: Effects of Viral Capsid and Genome Size. J. Virol. 76: 7651-7660 [Abstract] [Full Text]
Walters, R. W., Pilewski, J. M., Chiorini, J. A., Zabner, J. (2002). Secreted and Transmembrane Mucins Inhibit Gene Transfer with AAV4 More Efficiently than AAV5. J. Biol. Chem. 277: 23709-23713 [Abstract] [Full Text]
Rabinowitz, J. E., Rolling, F., Li, C., Conrath, H., Xiao, W., Xiao, X., Samulski, R. J. (2002). Cross-Packaging of a Single Adeno-Associated Virus (AAV) Type 2 Vector Genome into Multiple AAV Serotypes Enables Transduction with Broad Specificity. J. Virol. 76: 791-801 [Abstract] [Full Text]
Ding, C., Urabe, M., Bergoin, M., Kotin, R. M. (2002). Biochemical Characterization of Junonia coenia Densovirus Nonstructural Protein NS-1. J. Virol. 76: 338-345 [Abstract] [Full Text]
Auricchio, A., Kobinger, G., Anand, V., Hildinger, M., O'Connor, E., Maguire, A. M., Wilson, J. M., Bennett, J. (2001). Exchange of surface proteins impacts on viral vector cellular specificity and transduction characteristics: the retina as a model. Hum Mol Genet 10: 3075-3081 [Abstract] [Full Text]
Duan, D., Yan, Z., Yue, Y., Ding, W., Engelhardt, J. F. (2001). Enhancement of Muscle Gene Delivery with Pseudotyped Adeno-Associated Virus Type 5 Correlates with Myoblast Differentiation. J. Virol. 75: 7662-7671 [Abstract] [Full Text]
Kaludov, N., Brown, K. E., Walters, R. W., Zabner, J., Chiorini, J. A. (2001). Adeno-Associated Virus Serotype 4 (AAV4) and AAV5 Both Require Sialic Acid Binding for Hemagglutination and Efficient Transduction but Differ in Sialic Acid Linkage Specificity. J. Virol. 75: 6884-6893 [Abstract] [Full Text]
Halbert, C. L., Allen, J. M., Miller, A. D. (2001). Adeno-Associated Virus Type 6 (AAV6) Vectors Mediate Efficient Transduction of Airway Epithelial Cells in Mouse Lungs Compared to That of AAV2 Vectors. J. Virol. 75: 6615-6624 [Abstract] [Full Text]
Hildinger, M., Auricchio, A., Gao, G., Wang, L., Chirmule, N., Wilson, J. M. (2001). Hybrid Vectors Based on Adeno-Associated Virus Serotypes 2 and 5 for Muscle-Directed Gene Transfer. J. Virol. 75: 6199-6203 [Abstract] [Full Text]
Yoon, M., Smith, D. H., Ward, P., Medrano, F. J., Aggarwal, A. K., Linden, R. M. (2001). Amino-Terminal Domain Exchange Redirects Origin-Specific Interactions of Adeno-Associated Virus Rep78 In Vitro. J. Virol. 75: 3230-3239 [Abstract] [Full Text]
Lukashov, V. V., Goudsmit, J. (2001). Evolutionary Relationships among Parvoviruses: Virus-Host Coevolution among Autonomous Primate Parvoviruses and Links between Adeno-Associated and Avian Parvoviruses. J. Virol. 75: 2729-2740 [Abstract] [Full Text]
Flotte, T., Agarwal, A., Wang, J., Song, S., Fenjves, E. S., Inverardi, L., Chesnut, K., Afione, S., Loiler, S., Wasserfall, C., Kapturczak, M., Ellis, T., Nick, H., Atkinson, M. (2001). Efficient Ex Vivo Transduction of Pancreatic Islet Cells With Recombinant Adeno-Associated Virus Vectors. Diabetes 50: 515-520 [Abstract] [Full Text]
Handa, A., Muramatsu, S.-i., Qiu, J., Mizukami, H., Brown, K. E. (2000). Adeno-associated virus (AAV)-3-based vectors transduce haematopoietic cells not susceptible to transduction with AAV-2-based vectors. J. Gen. Virol. 81: 2077-2084 [Abstract] [Full Text]
Zabner, J., Seiler, M., Walters, R., Kotin, R. M., Fulgeras, W., Davidson, B. L., Chiorini, J. A. (2000). Adeno-Associated Virus Type 5 (AAV5) but Not AAV2 Binds to the Apical Surfaces of Airway Epithelia and Facilitates Gene Transfer. J. Virol. 74: 3852-3858 [Abstract] [Full Text]
Walters, R. W., Duan, D., Engelhardt, J. F., Welsh, M. J. (2000). Incorporation of Adeno-Associated Virus in a Calcium Phosphate Coprecipitate Improves Gene Transfer to Airway Epithelia In Vitro and In Vivo. J. Virol. 74: 535-540 [Abstract] [Full Text]
Gavin, D. K., Young, S. M. Jr., Xiao, W., Temple, B., Abernathy, C. R., Pereira, D. J., Muzyczka, N., Samulski, R. J. (1999). Charge-to-Alanine Mutagenesis of the Adeno-Associated Virus Type 2 Rep78/68 Proteins Yields Temperature-Sensitive and Magnesium-Dependent Variants. J. Virol. 73: 9433-9445 [Abstract] [Full Text]
Russell, D. W., Kay, M. A. (1999). Adeno-Associated Virus Vectors and Hematology. Blood 94: 864-874 [Full Text]
Chiorini, J. A., Afione, S., Kotin, R. M. (1999). Adeno-Associated Virus (AAV) Type 5�Rep Protein Cleaves a Unique Terminal Resolution Site Compared with Other AAV Serotypes. J. Virol. 73: 4293-4298 [Abstract] [Full Text]
Walters, R. W., Yi, S. M. P., Keshavjee, S., Brown, K. E., Welsh, M. J., Chiorini, J. A., Zabner, J. (2001). Binding of Adeno-associated Virus Type 5 to 2,3-Linked Sialic Acid Is Required for Gene Transfer. J. Biol. Chem. 276: 20610-20616 [Abstract] [Full Text]
Davidson, B. L., Stein, C. S., Heth, J. A., Martins, I., Kotin, R. M., Derksen, T. A., Zabner, J., Ghodsi, A., Chiorini, J. A. (2000). From the Cover: Recombinant adeno-associated virus type 2, 4, and 5 vectors: Transduction of variant cell types and regions in the mammalian central nervous system. Proc. Natl. Acad. Sci. USA 97: 3428-3432 [Abstract] [Full Text]

J. Bacteriol.	Mol. Cell. Biol.	Microbiol. Mol. Biol. Rev.

Clin. Vaccine Immunol.	ALL ASM JOURNALS