This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bassett, S. E.
Right arrow Articles by Lanford, R. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bassett, S. E.
Right arrow Articles by Lanford, R. E.

 Previous Article  |  Next Article 

Journal of Virology, February 1999, p. 1118-1126, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Viral Persistence, Antibody to E1 and E2, and Hypervariable Region 1 Sequence Stability in Hepatitis C Virus-Inoculated Chimpanzees

Suzanne E. Bassett,1,2 David L. Thomas,3 Kathleen M. Brasky,4 and Robert E. Lanford1,2,*

Department of Virology and Immunology1 and Department of Laboratory and Animal Medicine,4 Southwest Foundation for Biomedical Research, San Antonio, Texas 78227; Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 782842; and Division of Infectious Diseases, The Johns Hopkins School of Medicine, Baltimore, Maryland3

Received 22 May 1998/Accepted 26 October 1998

The relationship of viral persistence, the immune response to hepatitis C virus (HCV) envelope proteins, and envelope sequence variability was examined in chimpanzees. Antibody reactivity to the HCV envelope proteins E1 or E2 was detected by enzyme-linked immunosorbent assay (ELISA) in more than 90% of a human serum panel. Although the ELISAs appeared to be sensitive indicators of HCV infection in human serum panels, the results of a cross-sectional study revealed that a low percentage of HCV-inoculated chimpanzees had detectable antibody to E1 (22%) and E2 (15%). Viral clearance, which was recognized in 28 (61%) of the chimpanzees, was not associated with an antibody response to E1 or E2. On the contrary, antibody to E2 was observed only in viremic chimpanzees. A longitudinal study of animals that cleared the viral infection or became chronically infected confirmed the low level of antibody to E1, E2, and the HVR-1. In 10 chronically infected animals, the sequence variation in the E2 hypervariable region (HVR-1) was minimal and did not coincide with antibody to E2 or to the HVR-1. In addition, low nucleotide and amino acid sequence variation was observed in the E1 and E2 regions from two chronically infected chimpanzees. These results suggest that mechanisms in addition to the emergence of HVR-1 antibody escape variants are involved in maintaining viral persistence. The significance of antibodies to E1 and E2 in the chimpanzee animal model is discussed.

* Corresponding author. Mailing address: Department of Virology and Immunology, Southwest Foundation for Biomedical Research, 7620 N.W. Loop 410, San Antonio, TX 78228. Phone: (210) 670-3245. Fax: (210) 670-3329. E-mail: rlanford{at}icarus.sfbr.org.

Journal of Virology, February 1999, p. 1118-1126, Vol. 73, No. 2
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ait-Goughoulte, M., Kanda, T., Meyer, K., Ryerse, J. S., Ray, R. B., Ray, R. (2008). Hepatitis C Virus Genotype 1a Growth and Induction of Autophagy. J. Virol. 82: 2241-2249 [Abstract] [Full Text]  
  • Wrobel, B., Torres-Puente, M., Jimenez, N., Bracho, M. A., Garcia-Robles, I., Moya, A., Gonzalez-Candelas, F. (2006). Analysis of the Overdispersed Clock in the Short-Term Evolution of Hepatitis C Virus: Using the E1/E2 Gene Sequences to Infer Infection Dates in a Single Source Outbreak. Mol Biol Evol 23: 1242-1253 [Abstract] [Full Text]  
  • Nainan, O. V., Lu, L., Gao, F.-X., Meeks, E., Robertson, B. H., Margolis, H. S. (2006). Selective transmission of hepatitis C virus genotypes and quasispecies in humans and experimentally infected chimpanzees. J. Gen. Virol. 87: 83-91 [Abstract] [Full Text]  
  • Meunier, J.-C., Engle, R. E., Faulk, K., Zhao, M., Bartosch, B., Alter, H., Emerson, S. U., Cosset, F.-L., Purcell, R. H., Bukh, J. (2005). Evidence for cross-genotype neutralization of hepatitis C virus pseudo-particles and enhancement of infectivity by apolipoprotein C1. Proc. Natl. Acad. Sci. USA 102: 4560-4565 [Abstract] [Full Text]  
  • Girard, C., Ravallec, M., Mariller, M., Bossy, J.-P., Cahour, A., Lopez-Ferber, M., Devauchelle, G., Inchauspe, G., Duonor-Cerutti, M. (2004). Effect of the 5' non-translated region on self-assembly of hepatitis C virus genotype 1a structural proteins produced in insect cells. J. Gen. Virol. 85: 3659-3670 [Abstract] [Full Text]  
  • Logvinoff, C., Major, M. E., Oldach, D., Heyward, S., Talal, A., Balfe, P., Feinstone, S. M., Alter, H., Rice, C. M., McKeating, J. A. (2004). Neutralizing antibody response during acute and chronic hepatitis C virus infection. Proc. Natl. Acad. Sci. USA 101: 10149-10154 [Abstract] [Full Text]  
  • Martin, A., Bodola, F., Sangar, D. V., Goettge, K., Popov, V., Rijnbrand, R., Lanford, R. E., Lemon, S. M. (2003). Chronic hepatitis associated with GB virus B persistence in a tamarin after intrahepatic inoculation of synthetic viral RNA. Proc. Natl. Acad. Sci. USA 100: 9962-9967 [Abstract] [Full Text]  
  • Henderson, D. K. (2003). Managing Occupational Risks for Hepatitis C Transmission in the Health Care Setting. Clin. Microbiol. Rev. 16: 546-568 [Abstract] [Full Text]  
  • Bergqvist, A., Sundstrom, S., Dimberg, L. Y., Gylfe, E., Masucci, M. G. (2003). The Hepatitis C Virus Core Protein Modulates T Cell Responses by Inducing Spontaneous and Altering T-cell Receptor-triggered Ca2+ Oscillations. J. Biol. Chem. 278: 18877-18883 [Abstract] [Full Text]  
  • Thomson, M., Nascimbeni, M., Havert, M. B., Major, M., Gonzales, S., Alter, H., Feinstone, S. M., Murthy, K. K., Rehermann, B., Liang, T. J. (2002). The Clearance of Hepatitis C Virus Infection in Chimpanzees May Not Necessarily Correlate with the Appearance of Acquired Immunity. J. Virol. 77: 862-870 [Abstract] [Full Text]  
  • Bigger, C. B., Brasky, K. M., Lanford, R. E. (2001). DNA Microarray Analysis of Chimpanzee Liver during Acute Resolving Hepatitis C Virus Infection. J. Virol. 75: 7059-7066 [Abstract] [Full Text]  
  • Weiner, A. J., Paliard, X., Selby, M. J., Medina-Selby, A., Coit, D., Nguyen, S., Kansopon, J., Arian, C. L., Ng, P., Tucker, J., Lee, C.-T., Polakos, N. K., Han, J., Wong, S., Lu, H.-H., Rosenberg, S., Brasky, K. M., Chien, D., Kuo, G., Houghton, M. (2001). Intrahepatic Genetic Inoculation of Hepatitis C Virus RNA Confers Cross-Protective Immunity. J. Virol. 75: 7142-7148 [Abstract] [Full Text]  
  • Lanford, R. E., Lee, H., Chavez, D., Guerra, B., Brasky, K. M. (2001). Infectious cDNA clone of the hepatitis C virus genotype 1 prototype sequence. J. Gen. Virol. 82: 1291-1297 [Abstract] [Full Text]  
  • Lu, L., Nakano, T., Orito, E., Mizokami, M., Robertson, B. H. (2001). Evaluation of Accumulation of Hepatitis C Virus Mutations in a Chronically Infected Chimpanzee: Comparison of the Core, E1, HVR1, and NS5b Regions. J. Virol. 75: 3004-3009 [Abstract] [Full Text]  
  • Bergqvist, A., Rice, C. M. (2001). Transcriptional Activation of the Interleukin-2 Promoter by Hepatitis C Virus Core Protein. J. Virol. 75: 772-781 [Abstract] [Full Text]  
  • Ray, S. C., Mao, Q., Lanford, R. E., Bassett, S., Laeyendecker, O., Wang, Y.-M., Thomas, D. L. (2000). Hypervariable Region 1 Sequence Stability during Hepatitis C Virus Replication in Chimpanzees. J. Virol. 74: 3058-3066 [Abstract] [Full Text]  
  • Kolykhalov, A. A., Mihalik, K., Feinstone, S. M., Rice, C. M. (2000). Hepatitis C Virus-Encoded Enzymatic Activities and Conserved RNA Elements in the 3' Nontranslated Region Are Essential for Virus Replication In Vivo. J. Virol. 74: 2046-2051 [Abstract] [Full Text]  
  • Liang, T. J., Rehermann, B., Seeff, L. B., Hoofnagle, J. H. (2000). Pathogenesis, Natural History, Treatment, and Prevention of Hepatitis C. ANN INTERN MED 132: 296-305 [Abstract] [Full Text]  
  • Feuer, R., Boone, J. D., Netski, D., Morzunov, S. P., St. Jeor, S. C. (1999). Temporal and Spatial Analysis of Sin Nombre Virus Quasispecies in Naturally Infected Rodents. J. Virol. 73: 9544-9554 [Abstract] [Full Text]  
  • Ray, S. C., Wang, Y.-M., Laeyendecker, O., Ticehurst, J. R., Villano, S. A., Thomas, D. L. (1999). Acute Hepatitis C Virus Structural Gene Sequences as Predictors of Persistent Viremia: Hypervariable Region 1 as a Decoy. J. Virol. 73: 2938-2946 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»