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Journal of Virology, December 1999, p. 9789-9795, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Latent Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus Interacts with RING3, a Homolog of the Drosophila Female Sterile Homeotic (fsh) Gene

Georgina M. Platt,1 Guy R. Simpson,1 Sibylle Mittnacht,2 and Thomas F. Schulz1,*

Molecular Virology Group, Department of Medical Microbiology and Genitourinary Medicine, The University of Liverpool, Liverpool L69 3GA,1 and Cell and Molecular Biology Section, The Institute of Cancer Research, Chester Beatty Laboratories, London SW3 6JB,2 United Kingdom

Received 28 May 1999/Accepted 10 August 1999

Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) is the likely infectious cause of Kaposi's sarcoma, primary effusion lymphoma, and some cases of multicentric Castleman's disease. Its latent nuclear antigen (LANA) is expressed in the nuclei of latently infected cells and may play a role in the persistence of episomal viral DNA in dividing cells. Here we report that LANA interacts with RING3, a nuclear protein and member of the Drosophila fsh (female sterile homeotic) family of proteins, some of which have previously been implicated in controlling gene expression. Binding of RING3 to LANA involves the ET domain, characteristic of fsh-related proteins, suggesting that this highly conserved region is involved in protein-protein interactions. The interaction between RING3 and LANA results in phosphorylation of serine and threonine residues located between amino acids 951 and 1107 in the carboxy-terminal region of LANA. However, RING3 is not itself a kinase but appears to recruit an as yet unidentified serine/threonine protein kinase into the complex which it forms with LANA.

* Corresponding author. Mailing address: Molecular Virology Group, Department of Medical Microbiology and Genitourinary Medicine, The University of Liverpool, Liverpool L69 3GA, United Kingdom. Phone: 44 151 706 4382. Fax: 44 151 706 5805. E-mail: tschulz{at}liv.ac.uk.

Journal of Virology, December 1999, p. 9789-9795, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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