This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shimoike, T.
Right arrow Articles by Miyamura, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shimoike, T.
Right arrow Articles by Miyamura, T.

 Previous Article  |  Next Article 

Journal of Virology, December 1999, p. 9718-9725, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Interaction of Hepatitis C Virus Core Protein with Viral Sense RNA and Suppression of Its Translation

Takashi Shimoike, Shigetaka Mimori, Hideki Tani, Yoshiharu Matsuura, and Tatsuo Miyamura*

Department of Virology II, National Institute of Infectious Diseases, Tokyo 162-8640, Japan

Received 14 May 1999/Accepted 18 August 1999

To clarify the binding properties of hepatitis C virus (HCV) core protein and its viral RNA for the encapsidation, morphogenesis, and replication of HCV, the specific interaction of HCV core protein with its genomic RNA synthesized in vitro was examined in an in vivo system. The positive-sense RNA from the 5' end to nucleotide (nt) 2327, which covers the 5' untranslated region (5'UTR) and a part of the coding region of HCV structural proteins, interacted with HCV core protein, while no interaction was observed in the same region of negative-sense RNA and in other regions of viral and antiviral sense RNAs. The internal ribosome entry site (IRES) exists around the 5'UTR of HCV; therefore, the interaction of the core protein with this region of HCV RNA suggests that there is some effect on its cap-independent translation. Cells expressing HCV core protein were transfected with reporter RNAs consisting of nt 1 to 709 of HCV RNA (the 5'UTR of HCV and about two-thirds of the core protein coding regions) followed by a firefly luciferase gene (HCV07Luc RNA). The translation of HCV07Luc RNA was suppressed in cells expressing the core protein, whereas no significant suppression was observed in the case of a reporter RNA possessing the IRES of encephalomyocarditis virus followed by a firefly luciferase. This suppression by the core protein occurred in a dose-dependent manner. The expression of the E1 envelope protein of HCV or beta -galactosidase did not suppress the translation of both HCV and EMCV reporter RNAs. We then examined the regions that are important for suppression of translation by the core protein and found that the region from nt 1 to 344 was enough to exert this suppression. These results suggest that the HCV core protein interacts with viral genomic RNA at a specific region to form nucleocapsids and regulates the expression of HCV by interacting with the 5'UTR.

* Corresponding author. Mailing address: Department of Virology II, National Institute of Infectious Diseases, 1-23-1, Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. Phone: 81-3-5285-1111. Fax: 81-3-5285-1161. E-mail: tmiyam{at}nih.go.jp.

Journal of Virology, December 1999, p. 9718-9725, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Xiao, M., Wang, Y., Zhu, Z., Yu, J., Wan, L., Chen, J. (2009). Influence of NS5A protein of classical swine fever virus (CSFV) on CSFV internal ribosome entry site-dependent translation. J. Gen. Virol. 90: 2923-2928 [Abstract] [Full Text]  
  • Friebe, P., Bartenschlager, R. (2009). Role of RNA Structures in Genome Terminal Sequences of the Hepatitis C Virus for Replication and Assembly. J. Virol. 83: 11989-11995 [Abstract] [Full Text]  
  • Yi, G., Letteney, E., Kim, C.-H., Kao, C. C. (2009). Brome mosaic virus capsid protein regulates accumulation of viral replication proteins by binding to the replicase assembly RNA element. RNA 15: 615-626 [Abstract] [Full Text]  
  • Jaffrelo, L., Chabas, S., Reigadas, S., Pflieger, A., Wychowski, C., Rumi, J., Ventura, M., Toulme, J.-J., Staedel, C. (2008). A functional selection of viral genetic elements in cultured cells to identify hepatitis C virus RNA translation inhibitors. Nucleic Acids Res 36: e95-e95 [Abstract] [Full Text]  
  • Steinmann, E., Brohm, C., Kallis, S., Bartenschlager, R., Pietschmann, T. (2008). Efficient trans-Encapsidation of Hepatitis C Virus RNAs into Infectious Virus-Like Particles. J. Virol. 82: 7034-7046 [Abstract] [Full Text]  
  • Wolf, M., Dimitrova, M., Baumert, T. F., Schuster, C. (2008). The major form of hepatitis C virus alternate reading frame protein is suppressed by core protein expression. Nucleic Acids Res 36: 3054-3064 [Abstract] [Full Text]  
  • Xiao, M., Bai, Y., Xu, H., Geng, X., Chen, J., Wang, Y., Chen, J., Li, B. (2008). Effect of NS3 and NS5B proteins on classical swine fever virus internal ribosome entry site-mediated translation and its host cellular translation. J. Gen. Virol. 89: 994-999 [Abstract] [Full Text]  
  • Murray, C. L., Jones, C. T., Tassello, J., Rice, C. M. (2007). Alanine Scanning of the Hepatitis C Virus Core Protein Reveals Numerous Residues Essential for Production of Infectious Virus. J. Virol. 81: 10220-10231 [Abstract] [Full Text]  
  • Miyamoto, H., Moriishi, K., Moriya, K., Murata, S., Tanaka, K., Suzuki, T., Miyamura, T., Koike, K., Matsuura, Y. (2007). Involvement of the PA28{gamma}-Dependent Pathway in Insulin Resistance Induced by Hepatitis C Virus Core Protein. J. Virol. 81: 1727-1735 [Abstract] [Full Text]  
  • Nakai, K., Okamoto, T., Kimura-Someya, T., Ishii, K., Lim, C. K., Tani, H., Matsuo, E., Abe, T., Mori, Y., Suzuki, T., Miyamura, T., Nunberg, J. H., Moriishi, K., Matsuura, Y. (2006). Oligomerization of Hepatitis C Virus Core Protein Is Crucial for Interaction with the Cytoplasmic Domain of E1 Envelope Protein. J. Virol. 80: 11265-11273 [Abstract] [Full Text]  
  • Li, P. W.-L., Li, J., Timmerman, S. L., Krushel, L. A., Martin, S. L. (2006). The dicistronic RNA from the mouse LINE-1 retrotransposon contains an internal ribosome entry site upstream of each ORF: implications for retrotransposition. Nucleic Acids Res 34: 853-864 [Abstract] [Full Text]  
  • Ivanyi-Nagy, R., Kanevsky, I., Gabus, C., Lavergne, J.-P., Ficheux, D., Penin, F., Fosse, P., Darlix, J.-L. (2006). Analysis of hepatitis C virus RNA dimerization and core-RNA interactions.. Nucleic Acids Res 34: 2618-2633 [Abstract] [Full Text]  
  • Zhang, J., Yamada, O., Sakamoto, T., Yoshida, H., Araki, H., Shimotohno, K. (2005). Exploiting cis-Acting Replication Elements To Direct Hepatitis C Virus-Dependent Transgene Expression. J. Virol. 79: 5923-5932 [Abstract] [Full Text]  
  • Boni, S., Lavergne, J.-P., Boulant, S., Cahour, A. (2005). Hepatitis C Virus Core Protein Acts as a trans-Modulating Factor on Internal Translation Initiation of the Viral RNA. J. Biol. Chem. 280: 17737-17748 [Abstract] [Full Text]  
  • Kalliampakou, K. I., Kalamvoki, M., Mavromara, P. (2005). Hepatitis C virus (HCV) NS5A protein downregulates HCV IRES-dependent translation. J. Gen. Virol. 86: 1015-1025 [Abstract] [Full Text]  
  • Suzuki, R., Sakamoto, S., Tsutsumi, T., Rikimaru, A., Tanaka, K., Shimoike, T., Moriishi, K., Iwasaki, T., Mizumoto, K., Matsuura, Y., Miyamura, T., Suzuki, T. (2005). Molecular Determinants for Subcellular Localization of Hepatitis C Virus Core Protein. J. Virol. 79: 1271-1281 [Abstract] [Full Text]  
  • Girard, C., Ravallec, M., Mariller, M., Bossy, J.-P., Cahour, A., Lopez-Ferber, M., Devauchelle, G., Inchauspe, G., Duonor-Cerutti, M. (2004). Effect of the 5' non-translated region on self-assembly of hepatitis C virus genotype 1a structural proteins produced in insect cells. J. Gen. Virol. 85: 3659-3670 [Abstract] [Full Text]  
  • Ogino, T., Fukuda, H., Imajoh-Ohmi, S., Kohara, M., Nomoto, A. (2004). Membrane Binding Properties and Terminal Residues of the Mature Hepatitis C Virus Capsid Protein in Insect Cells. J. Virol. 78: 11766-11777 [Abstract] [Full Text]  
  • Li, D., Lott, W. B., Martyn, J., Haqshenas, G., Gowans, E. J. (2004). Differential Effects on the Hepatitis C Virus (HCV) Internal Ribosome Entry Site by Vitamin B12 and the HCV Core Protein. J. Virol. 78: 12075-12081 [Abstract] [Full Text]  
  • Wang, S.-H., Syu, W.-J., Hu, S.-T. (2004). Identification of the homotypic interaction domain of the core protein of dengue virus type 2. J. Gen. Virol. 85: 2307-2314 [Abstract] [Full Text]  
  • Cristofari, G., Ivanyi-Nagy, R., Gabus, C., Boulant, S., Lavergne, J.-P., Penin, F., Darlix, J.-L. (2004). The hepatitis C virus Core protein is a potent nucleic acid chaperone that directs dimerization of the viral (+) strand RNA in vitro. Nucleic Acids Res 32: 2623-2631 [Abstract] [Full Text]  
  • Kronke, J., Kittler, R., Buchholz, F., Windisch, M. P., Pietschmann, T., Bartenschlager, R., Frese, M. (2004). Alternative Approaches for Efficient Inhibition of Hepatitis C Virus RNA Replication by Small Interfering RNAs. J. Virol. 78: 3436-3446 [Abstract] [Full Text]  
  • Meignin, C., Bailly, J.-L., Arnaud, F., Dastugue, B., Vaury, C. (2003). The 5' Untranslated Region and Gag product of Idefix, a Long Terminal Repeat-Retrotransposon from Drosophila melanogaster, Act Together To Initiate a Switch between Translated and Untranslated States of the Genomic mRNA. Mol. Cell. Biol. 23: 8246-8254 [Abstract] [Full Text]  
  • Moriishi, K., Okabayashi, T., Nakai, K., Moriya, K., Koike, K., Murata, S., Chiba, T., Tanaka, K., Suzuki, R., Suzuki, T., Miyamura, T., Matsuura, Y. (2003). Proteasome Activator PA28{gamma}-Dependent Nuclear Retention and Degradation of Hepatitis C Virus Core Protein. J. Virol. 77: 10237-10249 [Abstract] [Full Text]  
  • VYAS, J., ELIA, A., CLEMENS, M. J. (2003). Inhibition of the protein kinase PKR by the internal ribosome entry site of hepatitis C virus genomic RNA. RNA 9: 858-870 [Abstract] [Full Text]  
  • Imbert, I., Dimitrova, M., Kien, F., Kieny, M. P., Schuster, C. (2003). Hepatitis C virus IRES efficiency is unaffected by the genomic RNA 3'NTR even in the presence of viral structural or non-structural proteins. J. Gen. Virol. 84: 1549-1557 [Abstract] [Full Text]  
  • Li, D., Takyar, S. T., Lott, W. B., Gowans, E. J. (2003). Amino acids 1-20 of the hepatitis C virus (HCV) core protein specifically inhibit HCV IRES-dependent translation in HepG2 cells, and inhibit both HCV IRES- and cap-dependent translation in HuH7 and CV-1 cells. J. Gen. Virol. 84: 815-825 [Abstract] [Full Text]  
  • Chen, S.-Y., Kao, C.-F., Chen, C.-M., Shih, C.-M., Hsu, M.-J., Chao, C.-H., Wang, S.-H., You, L.-R., Lee, Y.-H. W. (2003). Mechanisms for Inhibition of Hepatitis B Virus Gene Expression and Replication by Hepatitis C Virus Core Protein. J. Biol. Chem. 278: 591-607 [Abstract] [Full Text]  
  • Ma, H.-C., Ke, C.-H., Hsieh, T.-Y., Lo, S.-Y. (2002). The first hydrophobic domain of the hepatitis C virus E1 protein is important for interaction with the capsid protein. J. Gen. Virol. 83: 3085-3092 [Abstract] [Full Text]  
  • Wang, S.-H., Syu, W.-J., Huang, K.-J., Lei, H.-Y., Yao, C.-W., King, C.-C., Hu, S.-T. (2002). Intracellular localization and determination of a nuclear localization signal of the core protein of dengue virus. J. Gen. Virol. 83: 3093-3102 [Abstract] [Full Text]  
  • Isoyama, T., Kuge, S., Nomoto, A. (2002). The Core Protein of Hepatitis C Virus Is Imported into the Nucleus by Transport Receptor Kap123p but Inhibits Kap121p-dependent Nuclear Import of Yeast AP1-like Transcription Factor in Yeast Cells. J. Biol. Chem. 277: 39634-39641 [Abstract] [Full Text]  
  • Clayton, R. F., Owsianka, A., Aitken, J., Graham, S., Bhella, D., Patel, A. H. (2002). Analysis of Antigenicity and Topology of E2 Glycoprotein Present on Recombinant Hepatitis C Virus-Like Particles. J. Virol. 76: 7672-7682 [Abstract] [Full Text]  
  • Blanchard, E., Brand, D., Trassard, S., Goudeau, A., Roingeard, P. (2002). Hepatitis C Virus-Like Particle Morphogenesis. J. Virol. 76: 4073-4079 [Abstract] [Full Text]  
  • Lu, W., Strohecker, A., Ou, J.-h. (2001). Post-translational Modification of the Hepatitis C Virus Core Protein by Tissue Transglutaminase. J. Biol. Chem. 276: 47993-47999 [Abstract] [Full Text]  
  • Gigou, M., Roque-Afonso, A. M., Falissard, B., Penin, F., Dussaix, E., Feray, C. (2001). Genetic Clustering of Hepatitis C Virus Strains and Severity of Recurrent Hepatitis after Liver Transplantation. J. Virol. 75: 11292-11297 [Abstract] [Full Text]  
  • Lott, W. B., Takyar, S. S., Tuppen, J., Crawford, D. H. G., Harrison, M., Sloots, T. P., Gowans, E. J. (2001). Vitamin B12 and hepatitis C: Molecular biology and human pathology. Proc. Natl. Acad. Sci. USA 10.1073/pnas.081072798v1 [Abstract] [Full Text]  
  • Kunkel, M., Lorinczi, M., Rijnbrand, R., Lemon, S. M., Watowich, S. J. (2001). Self-Assembly of Nucleocapsid-Like Particles from Recombinant Hepatitis C Virus Core Protein. J. Virol. 75: 2119-2129 [Abstract] [Full Text]  
  • Wang, T.-H., Rijnbrand, R. C. A., Lemon, S. M. (2000). Core Protein-Coding Sequence, but Not Core Protein, Modulates the Efficiency of Cap-Independent Translation Directed by the Internal Ribosome Entry Site of Hepatitis C Virus. J. Virol. 74: 11347-11358 [Abstract] [Full Text]  
  • Bartenschlager, R., Lohmann, V. (2000). Replication of hepatitis C virus. J. Gen. Virol. 81: 1631-1648 [Full Text]  
  • Lott, W. B., Takyar, S. S., Tuppen, J., Crawford, D. H. G., Harrison, M., Sloots, T. P., Gowans, E. J. (2001). Vitamin B12 and hepatitis C: Molecular biology and human pathology. Proc. Natl. Acad. Sci. USA 98: 4916-4921 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»