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Journal of Virology, December 1999, p. 10546-10550, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of a Novel Sequence at the 3' End of the GB Virus B Genome

Andrea Sbardellati,1 Elisa Scarselli,1 Licia Tomei,1 Alexander S. Kekulé,2 and Cinzia Traboni1,*

Istituto di Ricerche di Biologia Molecolare P. Angeletti, 00040 Pomezia, Rome, Italy,1 and Institut für Medizinische Mikrobiologie, Martin-Luther-Universität Halle-Wittenberg, D-06097 Halle an der Saale, Germany2

Received 5 May 1999/Accepted 8 September 1999

GB virus B (GBV-B) is a virus of the family Flaviviridae that infects small primates (Saguinus sp. [tamarins]) and shows similarities to hepatitis C virus (HCV) in genome organization, protein function, tissue tropism, and pathogenicity. This suggests the possibility of using tamarins infected by GBV-B or GBV-B/HCV chimeric viruses as a surrogate animal model of HCV infection. To achieve the construction of such chimeric viruses, it is essential to produce a complete and infectious GBV-B genomic RNA. We have identified a novel sequence at the 3' end of the GBV-B genome and show that it can be arranged in a secondary structure resembling that of the 3' end of the HCV genome, which is known to be essential for infectivity.

* Corresponding author. Mailing address: Istituto di Ricerche di Biologia Molecolare P. Angeletti (IRBM), Via Pontina Km 30.600, 00040 Pomezia, Rome, Italy. Phone: (39.06)91093241. Fax: (39.06)91093225. E-mail: traboni{at}irbm.it.

Journal of Virology, December 1999, p. 10546-10550, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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