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Journal of Virology, December 1999, p. 10531-10535, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Open Reading Frame 1 of the Norwalk-Like Virus Camberwell: Completion of Sequence and Expression in Mammalian Cells

Ee Ling Seah,1 John A. Marshall,2 and Peter J. Wright1,*

Department of Microbiology, Monash University, Clayton, Victoria 3168,1 and Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria 3051,2 Australia

Received 11 May 1999/Accepted 27 August 1999

The ORF1 sequence was determined for Camberwell virus, a genogroup 2 Norwalk-like virus, completing the full genome of 7,555 nucleotides. ORF1 cDNA was cloned into a simian virus 40-based expression vector, and the viral proteins synthesized following transfection into COS cells were analyzed. By using antisera directed against the helicase, protease, or polymerase regions, eight polypeptides ranging in size from 19 to 117 kDa were detected by radioimmunoprecipitation. The cleavage sites determining the amino and carboxy termini of the 3C-like protease were identified at E1008/A and E1189/G, respectively.

* Corresponding author. Mailing address: Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia. Phone: 61 3 9905 4828. Fax: 61 3 9905 4811. E-mail: Peter.Wright{at}med.monash.edu.au.

Journal of Virology, December 1999, p. 10531-10535, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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