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Journal of Virology, December 1999, p. 10508-10513, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Cellular Motor Protein KIF-4 Associates with
Retroviral Gag
Yao
Tang,1
Ulrike
Winkler,1
Eric O.
Freed,2
Ted A.
Torrey,1
Wankee
Kim,3
Henry
Li,4
Stephen P.
Goff,4 and
Herbert C.
Morse III1,*
Laboratory of
Immunopathology1 and Laboratory of
Molecular Microbiology,2 National Institute of
Allergy and Infectious Diseases, National Institutes of Health,
Bethesda, Maryland 20892; Institute for Medical Science,
School of Medicine, Ajou University, Suwon,
Korea3; and Department of Biochemistry
and Molecular Biophysics, Columbia University College of Physicians
and Surgeons, New York, New York4
Received 26 March 1999/Accepted 24 August 1999
Previously we demonstrated that murine retroviral Gag proteins
associate with a cellular motor protein, KIF-4. Using the yeast two-hybrid assay, we also found an association of KIF-4 with Gag proteins of Mason-Pfizer monkey virus (MPMV), simian immunodeficiency virus (SIV), and human immunodeficiency virus type 1 (HIV-1). Studies
performed with mammalian cell systems confirmed that the HIV-1 Gag
protein associates with KIF-4. Soluble cytoplasmic proteins from cells
infected with recombinant vaccinia virus expressing the entire Gag-Pol
precursor protein of HIV-1 or transfected with HIV-1 molecular clone
pNL4-3 were fractionated by sucrose gradient centrifugation and further
separated by size-exclusion and anion-exchange chromatographies. KIF-4
and HIV-1 Gag cofractionated in both chromatographic separations.
Immunoprecipitation assays have also verified the KIF-4-Gag
association. KIF-4 binds mainly to the Gag precursor (Pr55 Gag) and a
matrix-capsid processing intermediate (Pr42) but not to other processed
Gag products. The binding of Gag is mediated by a domain of KIF-4
proximal to the C terminus. These results, and our previous studies,
raise the possibility that KIF-4 may play an important role in
retrovirus Gag protein transport.
*
Corresponding author. Mailing address: LIP,
NIAID, NIH, Building 7, Room 304, MSC 0760, Bethesda, MD 20892-0760. Phone: (301) 496-6379. Fax: (301) 402-0077. E-mail:
hm16c{at}nih.gov.
Journal of Virology, December 1999, p. 10508-10513, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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