Previous Article | Next Article 
Journal of Virology, December 1999, p. 10173-10182, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
A Single Rep Protein Initiates Replication of
Multiple Genome Components of Faba Bean Necrotic Yellows Virus, a
Single-Stranded DNA Virus of Plants
Tatiana
Timchenko,1,*
Françoise
de Kouchkovsky,1
Lina
Katul,2
Chantal
David,1
Heinrich Josef
Vetten,2 and
Bruno
Gronenborn1
Institut des Sciences Végétales,
CNRS, 91198 Gif sur Yvette, France,1 and
Biologische Bundesanstalt für Land und
Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie
und Biologische Sicherheit, D-38104 Braunschweig,
Germany2
Received 15 March 1999/Accepted 2 September 1999
Faba bean necrotic yellows virus (FBNYV) belongs to the
nanoviruses, plant viruses whose genome consists of multiple circular single-stranded DNA components. Eleven distinct DNAs, 5 of which encode
different replication initiator (Rep) proteins, have been identified in two FBNYV isolates. Origin-specific DNA cleavage and
nucleotidyl transfer activities were shown for Rep1 and Rep2 proteins
in vitro, and their essential tyrosine residues that catalyze these reactions were identified by site-directed mutagenesis. In addition, we showed that Rep1 and Rep2 proteins
hydrolyze ATP, and by changing the key lysine residue in the proteins'
nucleoside triphosphate binding sites, demonstrated that this ATPase
activity is essential for multiplication of virus DNA in vivo. Each of the five FBNYV Rep proteins initiated replication of the DNA molecule by which it was encoded, but only Rep2 was able to initiate replication of all the six other genome components. Furthermore, of the five rep components, only the Rep2-encoding DNA was always
detected in 55 FBNYV samples from eight countries. These data
provide experimental evidence for a master replication protein encoded
by a multicomponent single-stranded DNA virus.
*
Corresponding author. Mailing address: Institut des
Sciences Végétales, CNRS, 91198 Gif sur Yvette, France.
Phone: 33 1 69 82 38 13. Fax: 33 1 69 82 36 95. E-mail:
taniat{at}isv.cnrs-gif.fr.
Journal of Virology, December 1999, p. 10173-10182, Vol. 73, No. 12
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Grigoras, I., Timchenko, T., Gronenborn, B.
(2008). Transcripts encoding the nanovirus master replication initiator proteins are terminally redundant. J. Gen. Virol.
89: 583-593
[Abstract]
[Full Text]
-
Steinfeldt, T., Finsterbusch, T., Mankertz, A.
(2007). Functional Analysis of cis- and trans-Acting Replication Factors of Porcine Circovirus Type 1. J. Virol.
81: 5696-5704
[Abstract]
[Full Text]
-
Lageix, S., Catrice, O., Deragon, J.-M., Gronenborn, B., Pelissier, T., Ramirez, B. C.
(2007). The Nanovirus-Encoded Clink Protein Affects Plant Cell Cycle Regulation through Interaction with the Retinoblastoma-Related Protein. J. Virol.
81: 4177-4185
[Abstract]
[Full Text]
-
Hu, J.-M., Fu, H.-C., Lin, C.-H., Su, H.-J., Yeh, H.-H.
(2007). Reassortment and Concerted Evolution in Banana Bunchy Top Virus Genomes. J. Virol.
81: 1746-1761
[Abstract]
[Full Text]
-
Herrera-Valencia, V. A., Dugdale, B., Harding, R. M., Dale, J. L.
(2006). An iterated sequence in the genome of Banana bunchy top virus is essential for efficient replication.. J. Gen. Virol.
87: 3409-3412
[Abstract]
[Full Text]
-
Steinfeldt, T., Finsterbusch, T., Mankertz, A.
(2006). Demonstration of nicking/joining activity at the origin of DNA replication associated with the rep and rep' proteins of porcine circovirus type 1.. J. Virol.
80: 6225-6234
[Abstract]
[Full Text]
-
Timchenko, T., Katul, L., Aronson, M., Vega-Arreguin, J. C., Ramirez, B. C., Vetten, H. J., Gronenborn, B.
(2006). Infectivity of nanovirus DNAs: induction of disease by cloned genome components of Faba bean necrotic yellows virus. J. Gen. Virol.
87: 1735-1743
[Abstract]
[Full Text]
-
Vega-Arreguin, J. C., Timchenko, T., Gronenborn, B., Ramirez, B. C.
(2005). A Functional Histidine-Tagged Replication Initiator Protein: Implications for the Study of Single-Stranded DNA Virus Replication In Planta. J. Virol.
79: 8422-8430
[Abstract]
[Full Text]
-
Saunders, K., Bedford, I. D., Stanley, J.
(2002). Adaptation from whitefly to leafhopper transmission of an autonomously replicating nanovirus-like DNA component associated with ageratum yellow vein disease. J. Gen. Virol.
83: 907-913
[Abstract]
[Full Text]
-
Horser, C. L., Harding, R. M., Dale, J. L.
(2001). Banana bunchy top nanovirus DNA-1 encodes the 'master' replication initiation protein. J. Gen. Virol.
82: 459-464
[Abstract]
[Full Text]
-
Merits, A., Fedorkin, O. N., Guo, D., Kalinina, N. O., Morozov, S. Yu.
(2000). Activities associated with the putative replication initiation protein of Coconut foliar decay virus, a tentative member of the genus Nanovirus. J. Gen. Virol.
81: 3099-3106
[Abstract]
[Full Text]
-
Saunders, K., Bedford, I. D., Briddon, R. W., Markham, P. G., Wong, S. M., Stanley, J.
(2000). A unique virus complex causes Ageratum yellow vein disease. Proc. Natl. Acad. Sci. USA
97: 6890-6895
[Abstract]
[Full Text]
-
Aronson, M. N., Meyer, A. D., Györgyey, J., Katul, L., Vetten, H. J., Gronenborn, B., Timchenko, T.
(2000). Clink, a Nanovirus-Encoded Protein, Binds both pRB and SKP1. J. Virol.
74: 2967-2972
[Abstract]
[Full Text]