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Journal of Virology, November 1999, p. 9679-9682, Vol. 73, No. 11
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rescue of Influenza A Virus from Recombinant DNA

Ervin Fodor,1,2 Louise Devenish,1 Othmar G. Engelhardt,2,dagger Peter Palese,2 George G. Brownlee,1,* and Adolfo García-Sastre2

Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom,1 and Department of Microbiology, Mount Sinai School of Medicine, New York, New York 100292

Received 13 July 1999/Accepted 5 August 1999

We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.

* Corresponding author. Mailing address: Chemical Pathology Unit, Sir William Dunn School of Pathology, University of Oxford, South Parks Rd., Oxford OX1 3RE, United Kingdom. Phone: 44 (01865) 275559. Fax: 44 (1865) 275556. E-mail: George.Brownlee{at}path.ox.ac.uk.

dagger Present address: Department of Virology, University of Freiburg, D-79008 Freiburg, Germany.

Journal of Virology, November 1999, p. 9679-9682, Vol. 73, No. 11
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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  • Bancroft, C. T., Parslow, T. G. (2002). Evidence for Segment-Nonspecific Packaging of the Influenza A Virus Genome. J. Virol. 76: 7133-7139 [Abstract] [Full Text]  
  • Portela, A., Digard, P. (2002). The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication. J. Gen. Virol. 83: 723-734 [Abstract] [Full Text]  
  • Duhaut, S. D., Dimmock, N. J. (2002). Defective segment 1 RNAs that interfere with production of infectious influenza A virus require at least 150 nucleotides of 5' sequence: evidence from a plasmid-driven system. J. Gen. Virol. 83: 403-411 [Abstract] [Full Text]  
  • Ferko, B., Stasakova, J., Sereinig, S., Romanova, J., Katinger, D., Niebler, B., Katinger, H., Egorov, A. (2001). Hyperattenuated Recombinant Influenza A Virus Nonstructural-Protein-Encoding Vectors Induce Human Immunodeficiency Virus Type 1 Nef-Specific Systemic and Mucosal Immune Responses in Mice. J. Virol. 75: 8899-8908 [Abstract] [Full Text]  
  • Wagner, E., Engelhardt, O. G., Gruber, S., Haller, O., Kochs, G. (2001). Rescue of Recombinant Thogoto Virus from Cloned cDNA. J. Virol. 75: 9282-9286 [Abstract] [Full Text]  
  • Goto, H., Wells, K., Takada, A., Kawaoka, Y. (2001). Plasminogen-Binding Activity of Neuraminidase Determines the Pathogenicity of Influenza A Virus. J. Virol. 75: 9297-9301 [Abstract] [Full Text]  
  • Schultz-Cherry, S., Dybdahl-Sissoko, N., Neumann, G., Kawaoka, Y., Hinshaw, V. S. (2001). Influenza Virus NS1 Protein Induces Apoptosis in Cultured Cells. J. Virol. 75: 7875-7881 [Abstract] [Full Text]  
  • Perez, D. R., Donis, R. O. (2001). Functional Analysis of PA Binding by Influenza A Virus PB1: Effects on Polymerase Activity and Viral Infectivity. J. Virol. 75: 8127-8136 [Abstract] [Full Text]  
  • Paragas, J., Talon, J., O'Neill, R. E., Anderson, D. K., Garcia-Sastre, A., Palese, P. (2001). Influenza B and C Virus NEP (NS2) Proteins Possess Nuclear Export Activities. J. Virol. 75: 7375-7383 [Abstract] [Full Text]  
  • Tourdot, S., Herath, S., Gould, K. G. (2001). Characterization of a new H-2Dk-restricted epitope prominent in primary influenza A virus infection. J. Gen. Virol. 82: 1749-1755 [Abstract] [Full Text]  
  • Watanabe, T., Watanabe, S., Ito, H., Kida, H., Kawaoka, Y. (2001). Influenza A Virus Can Undergo Multiple Cycles of Replication without M2 Ion Channel Activity. J. Virol. 75: 5656-5662 [Abstract] [Full Text]  
  • Yao, Y., Mingay, L. J., McCauley, J. W., Barclay, W. S. (2001). Sequences in Influenza A Virus PB2 Protein That Determine Productive Infection for an Avian Influenza Virus in Mouse and Human Cell Lines. J. Virol. 75: 5410-5415 [Abstract] [Full Text]  
  • Hughes, M. T., McGregor, M., Suzuki, T., Suzuki, Y., Kawaoka, Y. (2001). Adaptation of Influenza A Viruses to Cells Expressing Low Levels of Sialic Acid Leads to Loss of Neuraminidase Activity. J. Virol. 75: 3766-3770 [Abstract] [Full Text]  
  • Basler, C. F., Reid, A. H., Dybing, J. K., Janczewski, T. A., Fanning, T. G., Zheng, H., Salvatore, M., Perdue, M. L., Swayne, D. E., Garcia-Sastre, A., Palese, P., Taubenberger, J. K. (2001). From the Cover: Sequence of the 1918 pandemic influenza virus nonstructural gene (NS) segment and characterization of recombinant viruses bearing the 1918 NS genes. Proc. Natl. Acad. Sci. USA 98: 2746-2751 [Abstract] [Full Text]  
  • Flick, R., Pettersson, R. F. (2001). Reverse Genetics System for Uukuniemi Virus (Bunyaviridae): RNA Polymerase I-Catalyzed Expression of Chimeric Viral RNAs. J. Virol. 75: 1643-1655 [Abstract] [Full Text]  
  • Wang, X., Li, M., Zheng, H., Muster, T., Palese, P., Beg, A. A., García-Sastre, A. (2000). Influenza A Virus NS1 Protein Prevents Activation of NF-kappa B and Induction of Alpha/Beta Interferon. J. Virol. 74: 11566-11573 [Abstract] [Full Text]  
  • Hoffmann, E., Webster, R. G. (2000). Unidirectional RNA polymerase I-polymerase II transcription system for the generation of influenza A virus from eight plasmids. J. Gen. Virol. 81: 2843-2847 [Abstract] [Full Text]  


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