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Journal of Virology, November 1999, p. 9664-9668, Vol. 73, No. 11
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

rab5 GTPase Regulates Adenovirus Endocytosis

Tanja Rauma,1 Juha Tuukkanen,2 Jeffrey M. Bergelson,3 Gerene Denning,4 and Timo Hautala1,5,*

Department of Pharmacology and Toxicology and Biocenter Oulu,1 Department of Internal Medicine,5 and Department of Anatomy,2 University of Oulu, 90220 Oulu, Finland; Department of Internal Medicine, University of Iowa College of Medicine, and The Veterans Affairs Medical Center, Iowa City, Iowa4; and Division of Immunologic and Infectious Diseases, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 191043

Received 3 May 1999/Accepted 9 August 1999

Adenovirus interaction with alpha v integrins is important for virus entry. We have examined the effects of adenovirus attachment on intracellular signaling in HeLa cells, with an emphasis on pathways known to be activated following integrin interaction with other ligands. We found no evidence for [Ca2+]c-mediated signaling or for tyrosine phosphorylation of pp125FAK, p130CAS, and paxillin. However, adenovirus attachment is known to activate phosphatidylinositol-3 kinase, which in turn may regulate endocytosis via rab5 GTPase. We found that adenovirus uptake was increased by overexpression of wild-type rab5 and decreased by dominant-negative rab5. These results indicate a role for rab5 in adenovirus entry.

* Corresponding author. Mailing address: University of Oulu, Department of Pharmacology and Toxicology, Kajaanintie 52D, 90220 Oulu, Finland. Phone: (358)-(0)8-537 5240. Fax: (358)-(0)8-537 5247. E-mail: thautala{at}sun3.oulu.fi.

Journal of Virology, November 1999, p. 9664-9668, Vol. 73, No. 11
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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