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Journal of Virology, November 1999, p. 9632-9637, Vol. 73, No. 11
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Association of Murine Leukemia Virus Pol with Virions, Independent of Gag-Pol Expression

Gary L. Buchschacher Jr.,1 Lei Yu,2 Fukashi Murai,2,dagger Theodore Friedmann,2,* and Atsushi Miyanohara2

Division of Hematology/Oncology, Department of Medicine,1 and Department of Pediatrics,2 Center for Molecular Genetics, University of California---San Diego, La Jolla, California 92093-0634

Received 4 March 1999/Accepted 3 August 1999

During the replication cycle of murine leukemia virus (MLV), Pol is normally synthesized as part of a Gag-Pol fusion protein. In this study, the ability of free MLV Pol to be incorporated into virions was examined. When MLV Gag and MLV Pol were coexpressed from separate plasmids in cells, reverse transcriptase (RT) activity associated with Gag core particles at a slightly lower level than did RT activity generated from wild-type Gag-Pol expression. Particles produced in this manner were somewhat less infectious than those produced with wild-type Gag-Pol. A smaller amount of MLV Pol also associated with heterologous human immunodeficiency virus type 1 Gag cores.

* Corresponding author. Mailing address: Department of Pediatrics, Center for Molecular Genetics, University of California---San Diego, 9500 Gilman Dr., La Jolla, CA 92093-0634. Phone: (619) 534-4268. Fax: (619) 534-1422. E-mail: tfriedmann{at}ucsd.edu.

dagger Present address: Pioneer Bioscience Institute, Shinjuku-ku, Tokyo 160-0004, Japan.

Journal of Virology, November 1999, p. 9632-9637, Vol. 73, No. 11
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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