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Journal of Virology, November 1999, p. 9369-9376, Vol. 73, No. 11
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Amino Acid Changes at Positions 173 and 187 in the Human T-Cell Leukemia Virus Type 1 Surface Glycoprotein Induce Specific Neutralizing Antibodies

Sophie Blanchard,1 Thérèse Astier-Gin,1,* Béatrice Tallet,1,2 Daniel Moynet,3 Danielle Londos-Gagliardi,2 and Bernard Guillemain2

EP630 CNRS-Université Victor Ségalen Bordeaux 2, 33077 Bordeaux Cedex,1 and Laboratoire d'Immunologie3 and Laboratoire de Virologie,2 Université Victor Ségalen Bordeaux 2, 33076 Bordeaux Cedex, France

Received 29 March 1999/Accepted 23 July 1999

The nucleotide sequence of human T-cell leukemia virus type 1 (HTLV-1) is highly conserved, most strains sharing at least 95% sequence identity. This sequence conservation is also found in the viral env gene, which codes for the two envelope glycoproteins that play a major role in the induction of a protective immune response against the virus. However, recent reports have indicated that some variations in env sequences may induce incomplete cross-reactivity between HTLV-1 strains. To identify the amino acid changes that might be involved in the antigenicity of neutralizable epitopes, we constructed expression vectors coding for the envelope glycoproteins of two HTLV-1 isolates (2060 and 2072) which induced human antibodies with different neutralization patterns. The amino acid sequences of the envelope glycoproteins differed at four positions. Vectors coding for chimeric or point-mutated envelope proteins were derived from 2060 and 2072 HTLV-1 env genes. Syncytium formation induced by the wild-type or mutated envelope proteins was inhibited by human sera with different neutralizing specificities. We thus identified two amino acid changes, I173right-arrowV and A187right-arrowT, that play an important role in the antigenicity of neutralizable epitopes located in this region of the surface envelope glycoprotein.

* Corresponding author. Mailing address: EP630 CNRS-Université Victor Ségalen Bordeaux 2, IBGC, 1 rue Camille Saint Saëns, 33077 Bordeaux Cedex, France. Phone: (33) 05 56 99 90 24. Fax: (33) 05 56 99 90 57. E-mail: t.astier{at}ibgc.u-bordeaux2.fr.

Journal of Virology, November 1999, p. 9369-9376, Vol. 73, No. 11
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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