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Journal of Virology, November 1999, p. 9348-9361, Vol. 73, No. 11
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Transcriptional Activation by the Product of Open Reading Frame 50 of Kaposi's Sarcoma-Associated Herpesvirus Is Required for Lytic Viral Reactivation in B Cells

David M. Lukac, Jessica R. Kirshner, and Don Ganem*

Departments of Microbiology and Medicine and Howard Hughes Medical Institute, University of California, San Francisco, California 94143

Received 21 May 1999/Accepted 2 August 1999

Kaposi's sarcoma (KS)-associated herpesvirus (KSHV) is a lymphotropic virus strongly linked to the development of KS, an endothelial cell neoplasm frequent in persons with AIDS. Reactivation from latency in B cells is thought to be an important antecedent to viral spread to endothelial cells during KS pathogenesis. Earlier experiments have posited a role for the transcriptional activator encoded by KSHV open reading frame 50 (ORF50) in such reactivation, since ectopic overexpression of this protein induces reactivation in latently infected B cells. Here we have explored several aspects of the expression, structure, and function of this protein bearing on this role. The ORF50 gene is expressed very early in lytic reactivation, before several other genes implicated as candidate regulatory genes in related viruses, and its expression can upregulate their promoters in transient assays. The protein is extensively phosphorylated in vivo and bears numerous sites for phosphorylation by protein kinase C, activators of which are potent stimulators of lytic induction. The C terminus of the ORF50 protein contains a domain that can strongly activate transcription when targeted to DNA; deletion of this domain generates an allele that expresses a truncated protein which retains the ability to form multimers with full-length ORF50 and functions as a dominant-negative protein. Expression of this allele in latently infected cells ablates spontaneous reactivation from latency and strikingly suppresses viral replication induced by multiple stimuli, including phorbol ester, ionomycin, and sodium butyrate. These results indicate that the ORF50 gene product plays an essential role in KSHV lytic replication and are consistent with its action as a putative molecular switch controlling the induction of virus from latency.

* Corresponding author. Mailing address: Departments of Microbiology and Medicine and Howard Hughes Medical Institute, Box 0414, University of California, San Francisco, CA 94143. Phone: (415) 476-2826. Fax: (415) 476-0939. E-mail: Ganem{at}socrates.ucsf.edu.

Journal of Virology, November 1999, p. 9348-9361, Vol. 73, No. 11
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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  • Brown, H. J., Song, M. J., Deng, H., Wu, T.-T., Cheng, G., Sun, R. (2003). NF-{kappa}B Inhibits Gammaherpesvirus Lytic Replication. J. Virol. 77: 8532-8540 [Abstract] [Full Text]  
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  • Caselli, E., Galvan, M., Santoni, F., Rotola, A., Caruso, A., Cassai, E., Luca, D. D. (2003). Human herpesvirus-8 (Kaposi's sarcoma-associated virus) ORF50 increases in vitro cell susceptibility to human immunodeficiency virus type 1 infection. J. Gen. Virol. 84: 1123-1131 [Abstract] [Full Text]  
  • Liao, W., Tang, Y., Lin, S.-F., Kung, H.-J., Giam, C.-Z. (2003). K-bZIP of Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 (KSHV/HHV-8) Binds KSHV/HHV-8 Rta and Represses Rta-Mediated Transactivation. J. Virol. 77: 3809-3815 [Abstract] [Full Text]  
  • Gwack, Y., Baek, H. J., Nakamura, H., Lee, S. H., Meisterernst, M., Roeder, R. G., Jung, J. U. (2003). Principal Role of TRAP/Mediator and SWI/SNF Complexes in Kaposi's Sarcoma-Associated Herpesvirus RTA-Mediated Lytic Reactivation. Mol. Cell. Biol. 23: 2055-2067 [Abstract] [Full Text]  
  • Jia, Q., Sun, R. (2003). Inhibition of Gammaherpesvirus Replication by RNA Interference. J. Virol. 77: 3301-3306 [Abstract] [Full Text]  
  • Wang, S. E., Wu, F. Y., Fujimuro, M., Zong, J., Hayward, S. D., Hayward, G. S. (2002). Role of CCAAT/Enhancer-Binding Protein Alpha (C/EBP{alpha}) in Activation of the Kaposi's Sarcoma-Associated Herpesvirus (KSHV) Lytic-Cycle Replication-Associated Protein (RAP) Promoter in Cooperation with the KSHV Replication and Transcription Activator (RTA) and RAP. J. Virol. 77: 600-623 [Abstract] [Full Text]  
  • Bowser, B. S., DeWire, S. M., Damania, B. (2002). Transcriptional Regulation of the K1 Gene Product of Kaposi's Sarcoma-Associated Herpesvirus. J. Virol. 76: 12574-12583 [Abstract] [Full Text]  
  • Ueda, K., Ishikawa, K., Nishimura, K., Sakakibara, S., Do, E., Yamanishi, K. (2002). Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Replication and Transcription Factor Activates the K9 (vIRF) Gene through Two Distinct cis Elements by a Non-DNA-Binding Mechanism. J. Virol. 76: 12044-12054 [Abstract] [Full Text]  
  • Lee, B.-S., Paulose-Murphy, M., Chung, Y.-H., Connlole, M., Zeichner, S., Jung, J. U. (2002). Suppression of Tetradecanoyl Phorbol Acetate-Induced Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus by K1 Signal Transduction. J. Virol. 76: 12185-12199 [Abstract] [Full Text]  
  • Lim, C., Sohn, H., Lee, D., Gwack, Y., Choe, J. (2002). Functional Dissection of Latency-Associated Nuclear Antigen 1 of Kaposi's Sarcoma-Associated Herpesvirus Involved in Latent DNA Replication and Transcription of Terminal Repeats of the Viral Genome. J. Virol. 76: 10320-10331 [Abstract] [Full Text]  
  • DeWire, S. M., McVoy, M. A., Damania, B. (2002). Kinetics of Expression of Rhesus Monkey Rhadinovirus (RRV) and Identification and Characterization of a Polycistronic Transcript Encoding the RRV Orf50/Rta, RRV R8, and R8.1 Genes. J. Virol. 76: 9819-9831 [Abstract] [Full Text]  
  • Deng, H., Chu, J. T., Rettig, M. B., Martinez-Maza, O., Sun, R. (2002). Rta of the human herpesvirus 8/Kaposi sarcoma-associated herpesvirus up-regulates human interleukin-6 gene expression. Blood 100: 1919-1921 [Abstract] [Full Text]  
  • Liang, Y., Chang, J., Lynch, S. J., Lukac, D. M., Ganem, D. (2002). The lytic switch protein of KSHV activates gene expression via functional interaction with RBP-Jkappa (CSL), the target of the Notch signaling pathway. Genes Dev. 16: 1977-1989 [Abstract] [Full Text]  
  • Deng, H., Song, M. J., Chu, J. T., Sun, R. (2002). Transcriptional Regulation of the Interleukin-6 Gene of Human Herpesvirus 8 (Kaposi's Sarcoma-Associated Herpesvirus). J. Virol. 76: 8252-8264 [Abstract] [Full Text]  
  • Tang, S., Zheng, Z.-M. (2002). Kaposi's Sarcoma-associated Herpesvirus K8 Exon 3 Contains Three 5'-Splice Sites and Harbors a K8.1 Transcription Start Site. J. Biol. Chem. 277: 14547-14556 [Abstract] [Full Text]  
  • Song, M. J., Li, X., Brown, H. J., Sun, R. (2002). Characterization of Interactions between RTA and the Promoter of Polyadenylated Nuclear RNA in Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus 8. J. Virol. 76: 5000-5013 [Abstract] [Full Text]  
  • Curreli, F., Cerimele, F., Muralidhar, S., Rosenthal, L. J., Cesarman, E., Friedman-Kien, A. E., Flore, O. (2002). Transcriptional Downregulation of ORF50/Rta by Methotrexate Inhibits the Switch of Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 from Latency to Lytic Replication. J. Virol. 76: 5208-5219 [Abstract] [Full Text]  
  • Chang, P.-J., Shedd, D., Gradoville, L., Cho, M.-S., Chen, L.-W., Chang, J., Miller, G. (2002). Open Reading Frame 50 Protein of Kaposi's Sarcoma-Associated Herpesvirus Directly Activates the Viral PAN and K12 Genes by Binding to Related Response Elements. J. Virol. 76: 3168-3178 [Abstract] [Full Text]  
  • Gwack, Y., Hwang, S., Lim, C., Won, Y. S., Lee, C. H., Choe, J. (2002). Kaposi's Sarcoma-associated Herpesvirus Open Reading Frame 50 Stimulates the Transcriptional Activity of STAT3. J. Biol. Chem. 277: 6438-6442 [Abstract] [Full Text]  
  • Wang, S., Liu, S., Wu, M.-H., Geng, Y., Wood, C. (2001). Identification of a Cellular Protein That Interacts and Synergizes with the RTA (ORF50) Protein of Kaposi's Sarcoma-Associated Herpesvirus in Transcriptional Activation. J. Virol. 75: 11961-11973 [Abstract] [Full Text]  
  • Pari, G. S., AuCoin, D., Colletti, K., Cei, S. A., Kirchoff, V., Wong, S. W. (2001). Identification of the Rhesus Macaque Rhadinovirus Lytic Origin of DNA Replication. J. Virol. 75: 11401-11407 [Abstract] [Full Text]  
  • Wu, T.-T., Tong, L., Rickabaugh, T., Speck, S., Sun, R. (2001). Function of Rta Is Essential for Lytic Replication of Murine Gammaherpesvirus 68. J. Virol. 75: 9262-9273 [Abstract] [Full Text]  


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