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Journal of Virology, November 1999, p. 9274-9283, Vol. 73, No. 11
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Human Cytomegalovirus 86-Kilodalton IE2 Protein Blocks Cell Cycle Progression in G1

Lüder Wiebusch and Christian Hagemeier*

Laboratory for Molecular Biology, Department of Pediatrics, Charité, Humboldt-University, D-10098 Berlin, Germany

Received 18 February 1999/Accepted 16 August 1999

The 86-kDa IE2 protein of human cytomegalovirus (HCMV) is an important regulator of viral and host cell gene expression. Still, besides its function as a transcription factor, little is known about the biological activities of IE2. Here, we show that IE2 can induce a G1 arrest in several different cell lines, including HCMV-permissive U-373 cells. The known transcriptional activation domains of IE2 are dispensable for G1 arrest, favoring a posttranscriptional mechanism mediating this cell cycle effect. We show that like human primary fibroblasts U-373 cells arrest in G1 upon infection with HCMV. This G1 arrest occurs within 24 h after infection and in proliferating cells depends on viral gene expression. Our data therefore suggest that IE2 is at least partially responsible for blocking the transition from G1 to S phase, which is induced when cells are infected with HCMV.

* Corresponding author. Mailing address: Laboratory for Molecular Biology, Department of Pediatrics, Charité, Humboldt-University, Ziegelstrasse 5-9, D-10098 Berlin, Germany. Phone: 49 30 2802 6433. Fax: 49 30 2802 6528. E-mail: christian.hagemeier{at}charite.de.

Journal of Virology, November 1999, p. 9274-9283, Vol. 73, No. 11
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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