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Journal of Virology, October 1999, p. 8857-8866, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Requirement for Cell-to-Cell Contact in
Epstein-Barr Virus Infection of Nasopharyngeal Carcinoma Cells
and Keratinocytes
Yao
Chang,1
Che-Huang
Tung,2
Yu-Tzu
Huang,1
Jean
Lu,1
Jen-Yang
Chen,1 and
Ching-Hwa
Tsai1,*
Graduate Institute of Microbiology, College
of Medicine,1 and Graduate Institute
of Fisheries Science,2 National Taiwan
University, Taipei, Taiwan, Republic of China
Received 22 April 1999/Accepted 6 July 1999
Two nasopharyngeal carcinoma (NPC) cell lines and one keratinocyte
cell line could be infected with Epstein-Barr virus (EBV) by
cocultivation with virus-producing cells but not by cell-free virus.
Using porous culture inserts to manipulate the cell-to-cell contact, we
demonstrated that contact between EBV donor B cells and EBV recipient
epithelial cells was required for the infection. Cell-to-cell contact
not only provided a CR2-independent route of infection but also
enhanced CR2-mediated infection in a synergistic manner. Activity of
two EBV promoters (Cp and Wp) and expression of EBNA2 were detected in
the infected population. A small proportion of the infected cells
spontaneously entered an EBV lytic state, which could be induced
prominently by chemical treatment. This study provides information on
how EBV may infect epithelial cells in vivo, such as at the onset of
NPC development.
*
Corresponding author. Mailing address: Graduate
Institute of Microbiology, College of Medicine, National Taiwan
University, Number 1, Section 1, Jen-Ai Road, Taipei, Taiwan, Republic
of China. Phone: 886-2-2397-0800, ext. 8298. Fax: 886-2-2391-5180. E-mail: chtsai{at}ha.mc.ntu.edu.tw.
Journal of Virology, October 1999, p. 8857-8866, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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