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Journal of Virology, October 1999, p. 8848-8850, Vol. 73, No. 10
0022-538X/99/$04.00+0

Evidence that the Genomic RNA of Hepatitis E Virus Is Capped

Yamina Kabrane-Lazizi,* Xiang-Jin Meng,dagger Robert H. Purcell, and Suzanne U. Emerson

Hepatitis Viruses and Molecular Hepatitis Sections, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892

Received 11 February 1999/Accepted 14 June 1999

Hepatitis E virus (HEV) is an unclassified virus with a positive-sense RNA genome and an undefined replication strategy. In order to determine whether the HEV genome is capped or not, we developed a reverse transcription-PCR assay that is based on the ability of a monoclonal antibody to recognize 7-methylguanosine (m7G). Antibody to m7G bound RNA extracted from virions of two different HEV genotypes. The cap analog competitively inhibited the binding of virion RNAs, demonstrating that HEV has a capped RNA genome.

* Corresponding author. Mailing address: Hepatitis Viruses and Molecular Hepatitis Sections, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892. Phone: (301) 496-6227. Fax: (301) 402-0524. E-mail: ykabrane{at}atlas.niaid.nih.gov.

dagger Present address: Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061.

Journal of Virology, October 1999, p. 8848-8850, Vol. 73, No. 10
0022-538X/99/$04.00+0


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