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Journal of Virology, October 1999, p. 8817-8823, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Localization of a Passively Transferred Human Recombinant Monoclonal Antibody to Herpes Simplex Virus Glycoprotein D to Infected Nerve Fibers and Sensory Neurons In Vivo

Pietro Paolo Sanna,1,* Thomas J. Deerinck,2 and Mark H. Ellisman2

Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 92037,1 and The National Center for Microscopy and Imaging Research, University of California at San Diego, La Jolla, California 920932

Received 21 May 1999/Accepted 6 July 1999

A human recombinant monoclonal antibody to herpes simplex virus (HSV) glycoprotein D labeled with the fluorescent dye Cy5 was administered to mice infected in the cornea with HSV type 1 (HSV-1). The distribution of such antibody in the corneas and trigeminal ganglia of the mice was then investigated by confocal microscopy. The antibody was detected on HSV-infected nerve fibers in the cornea---identified by colocalization with HSV antigens and the neuritic markers neurofilament, GAP-43, synapsin-1, and CNPase---and on the perikarya of sensory neurons in the HSV-1-infected neurons in ipsilateral trigeminal ganglia. Antibodies have been shown to be effective against many neurotropic viruses, often in the absence of obvious cell damage. Observations from experimental HSV infections suggest that antibodies could act in part by interfering with virus expression in the ganglia and/or with axonal spread. The present results provide morphological evidence of the localization of antiviral antibodies at anatomical sites relevant to such putative antibody-mediated protective actions and suggest that viral glycoproteins are accessible to antibodies on infected nerve fibers and sensory neurons.

* Corresponding author. Mailing address: Department of Neuropharmacology, The Scripps Research Institute, CVN12, La Jolla, CA 92037. Phone: (858) 784-7180. Fax: (858) 784-7393. E-mail: psanna{at}scripps.edu.

Journal of Virology, October 1999, p. 8817-8823, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Milligan, G. N., Meador, M. G., Chu, C.-F., Young, C. G., Martin, T. L., Bourne, N. (2005). Long-Term Presence of Virus-Specific Plasma Cells in Sensory Ganglia and Spinal Cord following Intravaginal Inoculation of Herpes Simplex Virus Type 2. J. Virol. 79: 11537-11540 [Abstract] [Full Text]  
  • Shimeld, C., Efstathiou, S., Hill, T. (2001). Tracking the Spread of a lacZ-Tagged Herpes Simplex Virus Type 1 between the Eye and the Nervous System of the Mouse: Comparison of Primary and Recurrent Infection. J. Virol. 75: 5252-5262 [Abstract] [Full Text]  
  • Sanna, P. P., Burton, D. R. (2000). Role of Antibodies in Controlling Viral Disease: Lessons from Experiments of Nature and Gene Knockouts. J. Virol. 74: 9813-9817 [Full Text]  


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