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Journal of Virology, October 1999, p. 8689-8695, Vol. 73, No. 10
Department of Internal Medicine, University
of Iowa College of Medicine, Iowa City, Iowa
52242,1 and Department of Gene
Therapy, Genzyme Corp., Framingham Massachusetts
017092
Received 16 December 1998/Accepted 21 June 1999
In studies of the genetic disease cystic fibrosis, recombinant
adenovirus type 2 (Ad2) and Ad5 are being investigated as vectors to
transfer cystic fibrosis transmembrane conductance regulator cDNA to
airway epithelia. However, earlier work has shown that human airway
epithelia are resistant to infection by Ad2 and Ad5. Therefore, we
examined the efficiency of other adenovirus serotypes at infecting
airway epithelia. We found that several serotypes of adenoviruses, in
particular, wild-type Ad17, infected a greater number of cells than
wild-type Ad2. The increased efficiency of wild-type Ad17 could be
explained by increased fiber-dependent binding to the epithelia.
Therefore, we constructed a chimeric virus, Ad2(17f)/
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
A Chimeric Type 2 Adenovirus Vector with a Type 17 Fiber Enhances Gene Transfer to Human Airway Epithelia
Gal-2, which is
identical to Ad2/
Gal-2 with the exception of having the fiber
protein of Ad17 replace Ad2 fiber. This vector retained the increased
binding and efficiency of gene transfer to well-differentiated human
airway epithelia. These data suggest that inclusion of Ad17 fiber into
adenovirus vectors may improve the outlook for gene delivery to human
airway epithelia.
*
Corresponding author. Mailing address: University of
Iowa College of Medicine, 500 EMRB, Iowa City, IA 52242. Phone: (319) 353-5511. Fax: (319) 353-5572. E-mail:
Joseph-Zabner{at}uiowa.edu.
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