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Journal of Virology, October 1999, p. 8559-8570, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Mutations within the Autographa californica Nucleopolyhedrovirus FP25K Gene Decrease the Accumulation of ODV-E66 and Alter Its Intranuclear Transport

Sharon C. Braunagel,1 Jared K. Burks,2 German Rosas-Acosta,2 Robert L. Harrison,2,3 H. Ma,4 and M. D. Summers1,2,4,*

Texas Agricultural Experiment Station,1 Department of Entomology,2 and Department of Biochemistry and Biophysics,4 Texas A&M University, College Station, Texas 77843-2475, and Department of Entomology, Iowa State University, Ames, Iowa 500113

Received 27 January 1999/Accepted 23 June 1999

Previous reports indicate that mutations within the Autographa californica nucleopolyhedrosis virus FP25K gene (open reading frame 61) significantly reduce incorporation of enveloped nucleocapsids into viral occlusions. We report that FP25K is a nucleocapsid protein of both the budded virus (BV) and occluded virus (ODV), and we describe the effects of two FP25K mutations (480-1 [N-terminal truncation] and FP-beta gal [C-terminal fusion]) on the expression and cellular localization of ODV-E66 and ODV-E25. Significantly decreased amounts of ODV-E66 are detected in cells infected with 480-1 or FP-beta gal viral mutants, even though during FP-beta gal infection, steady-state levels of ODV-E66 transcripts remain unchanged. While ODV-E66 is normally detected in intranuclear microvesicles and ODV envelopes by 24 h postinfection (p.i.), ODV-E66 remains cytosolic throughout infection in cells infected with 480-1 virus (up to 96 h p.i.), and its intranuclear localization is not detected until 96 h p.i. in cells infected with the FP-beta gal mutant virus. The nuclear localization of ODV-E25 is not affected during infection by the FP-beta gal mutant; however, its trafficking is significantly delayed during infection by the 480-1 mutant. Temporal Western blot analyses of cell lysates show that both 480-1 and FP-beta gal mutant virus infections result in altered accumulation patterns of several structural proteins, including gp67, BV/ODV-E26, and the major capsid protein p39. In addition to BV/ODV-E26, ODV-E66 and gp67 may interact with FP25K, and ODV-E25 and p39 may also be components of a protein complex containing ODV-E66 and FP25K. Together, these data suggest that FP25K and its associated protein complex(es) may play an important role in the targeting and intracellular transport of viral proteins during infection.

* Corresponding author. Mailing address: Texas A&M University, Department of Entomology, College Station, TX 77843-2475. Phone:(409) 847-9036. Fax: (409) 845-8934. E-mail: m-summers{at}tamu.edu.

Journal of Virology, October 1999, p. 8559-8570, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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