This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kern, F.
Right arrow Articles by Volk, H.-D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kern, F.
Right arrow Articles by Volk, H.-D.

 Previous Article  |  Next Article 

Journal of Virology, October 1999, p. 8179-8184, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Target Structures of the CD8+-T-Cell Response to Human Cytomegalovirus: the 72-Kilodalton Major Immediate-Early Protein Revisited

Florian Kern,1,* Ingolf Pascal Surel,1 Nicole Faulhaber,1,2 Claudia Frömmel,1 Jens Schneider-Mergener,1 Constanze Schönemann,3 Petra Reinke,2 and Hans-Dieter Volk1

Institut für Medizinische Immunologie, Medizinische Fakultät der Humboldt-Universität zu Berlin (Charité), 10098 Berlin,1 and Medizinische Klinik mit Schwerpunkt Hämatologie und Onkologie (Institut für Transfusionsmedizin und Immunhämatologie),3 and Medizinische Klinik mit Schwerpunkt Nephrologie und Intensivmedizin,2 Fakultät der Humboldt-Universität zu Berlin (Charité), 13353 Berlin, Germany

Received 26 April 1999/Accepted 28 June 1999

Cell-mediated immunity plays an essential role in the control of infection with the human cytomegalovirus (HCMV). However, only a few CD8+-T-cell epitopes are known, with the majority being contained in the pp65 phosphoprotein, which is believed to dominate the CD8+-T-cell response to HCMV. Here, we have readdressed the issue of CD8+ T cells specific for the 72-kDa major immediate-early protein (IE-1), which is nonstructural but is found very early and throughout the replicative cycle. Using a novel flow-cytometric assay, we were able to identify CD8+-T-cell epitopes (by IE-1 peptide-specific induction of cytokine synthesis) and simultaneously measure the frequency of cells directed against them. For this purpose, 81 pentadecamer peptides covering the complete 491-amino-acid sequence of IE-1 were tested on peripheral blood mononuclear cells of anti-HCMV immunoglobulin G-seropositive donors. At least 10 new epitopes were identified, and the fine specificity and presenting HLA molecule of the first of them was determined. The frequencies of CD8+ T cells directed against IE-1 were similar to those directed against pp65 in donors tested with known pp65-derived peptides. Importantly, additional testing of a corresponding set of peptides covering the complete sequence of pp65 on 10 of these donors identified individuals whose CD8+ T cells recognized IE-1 but not pp65 and vice versa, clearly illustrating that either protein may be a major target. In summary, our results suggest that IE-1 is far more important as a CD8+-T-cell target than current opinion suggests.

* Corresponding author. Mailing address: Institut für Medizinische Immunologie der Charité, Campus Mitte, COZ, 10098 Berlin, Germany. Phone: 030-28022858. Fax: 030-28025461. E-mail: florian.kern{at}charite.de.

Journal of Virology, October 1999, p. 8179-8184, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gras, S., Saulquin, X., Reiser, J.-B., Debeaupuis, E., Echasserieau, K., Kissenpfennig, A., Legoux, F., Chouquet, A., Le Gorrec, M., Machillot, P., Neveu, B., Thielens, N., Malissen, B., Bonneville, M., Housset, D. (2009). Structural Bases for the Affinity-Driven Selection of a Public TCR against a Dominant Human Cytomegalovirus Epitope. J. Immunol. 183: 430-437 [Abstract] [Full Text]  
  • Liu, J., Ruckwardt, T. J., Chen, M., Johnson, T. R., Graham, B. S. (2009). Characterization of Respiratory Syncytial Virus M- and M2-Specific CD4 T Cells in a Murine Model. J. Virol. 83: 4934-4941 [Abstract] [Full Text]  
  • Jagannathan, P., Osborne, C. M., Royce, C., Manion, M. M., Tilton, J. C., Li, L., Fischer, S., Hallahan, C. W., Metcalf, J. A., McLaughlin, M., Pipeling, M., McDyer, J. F., Manley, T. J., Meier, J. L., Altman, J. D., Hertel, L., Davey, R. T. Jr., Connors, M., Migueles, S. A. (2009). Comparisons of CD8+ T Cells Specific for Human Immunodeficiency Virus, Hepatitis C Virus, and Cytomegalovirus Reveal Differences in Frequency, Immunodominance, Phenotype, and Interleukin-2 Responsiveness. J. Virol. 83: 2728-2742 [Abstract] [Full Text]  
  • Noriega, V. M., Tortorella, D. (2009). Human Cytomegalovirus-Encoded Immune Modulators Partner To Downregulate Major Histocompatibility Complex Class I Molecules. J. Virol. 83: 1359-1367 [Abstract] [Full Text]  
  • Crough, T., Khanna, R. (2009). Immunobiology of Human Cytomegalovirus: from Bench to Bedside. Clin. Microbiol. Rev. 22: 76-98 [Abstract] [Full Text]  
  • Wilhelmi, V., Simon, C. O., Podlech, J., Bohm, V., Daubner, T., Emde, S., Strand, D., Renzaho, A., Lemmermann, N. A. W., Seckert, C. K., Reddehase, M. J., Grzimek, N. K. A. (2008). Transactivation of Cellular Genes Involved in Nucleotide Metabolism by the Regulatory IE1 Protein of Murine Cytomegalovirus Is Not Critical for Viral Replicative Fitness in Quiescent Cells and Host Tissues. J. Virol. 82: 9900-9916 [Abstract] [Full Text]  
  • Mersseman, V., Besold, K., Reddehase, M. J., Wolfrum, U., Strand, D., Plachter, B., Reyda, S. (2008). Exogenous introduction of an immunodominant peptide from the non-structural IE1 protein of human cytomegalovirus into the MHC class I presentation pathway by recombinant dense bodies. J. Gen. Virol. 89: 369-379 [Abstract] [Full Text]  
  • Vescovini, R., Biasini, C., Fagnoni, F. F., Telera, A. R., Zanlari, L., Pedrazzoni, M., Bucci, L., Monti, D., Medici, M. C., Chezzi, C., Franceschi, C., Sansoni, P. (2007). Massive Load of Functional Effector CD4+ and CD8+ T Cells against Cytomegalovirus in Very Old Subjects. J. Immunol. 179: 4283-4291 [Abstract] [Full Text]  
  • Day, E. K., Carmichael, A. J., ten Berge, I. J. M., Waller, E. C. P., Sissons, J. G. P., Wills, M. R. (2007). Rapid CD8+ T Cell Repertoire Focusing and Selection of High-Affinity Clones into Memory Following Primary Infection with a Persistent Human Virus: Human Cytomegalovirus. J. Immunol. 179: 3203-3213 [Abstract] [Full Text]  
  • Egli, A., Binggeli, S., Bodaghi, S., Dumoulin, A., Funk, G. A., Khanna, N., Leuenberger, D., Gosert, R., Hirsch, H. H. (2007). Cytomegalovirus and polyomavirus BK posttransplant. Nephrol Dial Transplant 22: viii72-viii82 [Abstract] [Full Text]  
  • Inokuma, M., dela Rosa, C., Schmitt, C., Haaland, P., Siebert, J., Petry, D., Tang, M., Suni, M. A., Ghanekar, S. A., Gladding, D., Dunne, J. F., Maino, V. C., Disis, M. L., Maecker, H. T. (2007). Functional T Cell Responses to Tumor Antigens in Breast Cancer Patients Have a Distinct Phenotype and Cytokine Signature. J. Immunol. 179: 2627-2633 [Abstract] [Full Text]  
  • Miles, D. J. C., van der Sande, M., Jeffries, D., Kaye, S., Ismaili, J., Ojuola, O., Sanneh, M., Touray, E. S., Waight, P., Rowland-Jones, S., Whittle, H., Marchant, A. (2007). Cytomegalovirus Infection in Gambian Infants Leads to Profound CD8 T-Cell Differentiation. J. Virol. 81: 5766-5776 [Abstract] [Full Text]  
  • Besold, K., Frankenberg, N., Pepperl-Klindworth, S., Kuball, J., Theobald, M., Hahn, G., Plachter, B. (2007). Processing and MHC class I presentation of human cytomegalovirus pp65-derived peptides persist despite gpUS2-11-mediated immune evasion. J. Gen. Virol. 88: 1429-1439 [Abstract] [Full Text]  
  • Khan, N., Best, D., Bruton, R., Nayak, L., Rickinson, A. B., Moss, P. A. H. (2007). T Cell Recognition Patterns of Immunodominant Cytomegalovirus Antigens in Primary and Persistent Infection. J. Immunol. 178: 4455-4465 [Abstract] [Full Text]  
  • Delmas, S., Martin, L., Baron, M., Nelson, J. A., Streblow, D. N., Davignon, J.-L. (2005). Optimization of CD4+ T Lymphocyte Response to Human Cytomegalovirus Nuclear IE1 Protein through Modifications of Both Size and Cellular Localization. J. Immunol. 175: 6812-6819 [Abstract] [Full Text]  
  • Trautmann, L., Rimbert, M., Echasserieau, K., Saulquin, X., Neveu, B., Dechanet, J., Cerundolo, V., Bonneville, M. (2005). Selection of T Cell Clones Expressing High-Affinity Public TCRs within Human Cytomegalovirus-Specific CD8 T Cell Responses. J. Immunol. 175: 6123-6132 [Abstract] [Full Text]  
  • Straathof, K. C., Leen, A. M., Buza, E. L., Taylor, G., Huls, M. H., Heslop, H. E., Rooney, C. M., Bollard, C. M. (2005). Characterization of Latent Membrane Protein 2 Specificity in CTL Lines from Patients with EBV-Positive Nasopharyngeal Carcinoma and Lymphoma. J. Immunol. 175: 4137-4147 [Abstract] [Full Text]  
  • Sylwester, A. W., Mitchell, B. L., Edgar, J. B., Taormina, C., Pelte, C., Ruchti, F., Sleath, P. R., Grabstein, K. H., Hosken, N. A., Kern, F., Nelson, J. A., Picker, L. J. (2005). Broadly targeted human cytomegalovirus-specific CD4+ and CD8+ T cells dominate the memory compartments of exposed subjects. JEM 202: 673-685 [Abstract] [Full Text]  
  • Sacre, K., Carcelain, G., Cassoux, N., Fillet, A.-M., Costagliola, D., Vittecoq, D., Salmon, D., Amoura, Z., Katlama, C., Autran, B. (2005). Repertoire, diversity, and differentiation of specific CD8 T cells are associated with immune protection against human cytomegalovirus disease. JEM 201: 1999-2010 [Abstract] [Full Text]  
  • Bunde, T., Kirchner, A., Hoffmeister, B., Habedank, D., Hetzer, R., Cherepnev, G., Proesch, S., Reinke, P., Volk, H.-D., Lehmkuhl, H., Kern, F. (2005). Protection from cytomegalovirus after transplantation is correlated with immediate early 1-specific CD8 T cells. JEM 201: 1031-1036 [Abstract] [Full Text]  
  • Trivedi, D., Williams, R. Y., O'Reilly, R. J., Koehne, G. (2005). Generation of CMV-specific T lymphocytes using protein-spanning pools of pp65-derived overlapping pentadecapeptides for adoptive immunotherapy. Blood 105: 2793-2801 [Abstract] [Full Text]  
  • Sadagopal, S., Amara, R. R., Montefiori, D. C., Wyatt, L. S., Staprans, S. I., Kozyr, N. L., McClure, H. M., Moss, B., Robinson, H. L. (2005). Signature for Long-Term Vaccine-Mediated Control of a Simian and Human Immunodeficiency Virus 89.6P Challenge: Stable Low-Breadth and Low-Frequency T-Cell Response Capable of Coproducing Gamma Interferon and Interleukin-2. J. Virol. 79: 3243-3253 [Abstract] [Full Text]  
  • Khan, N., Bruton, R., Taylor, G. S., Cobbold, M., Jones, T. R., Rickinson, A. B., Moss, P. A. H. (2005). Identification of Cytomegalovirus-Specific Cytotoxic T Lymphocytes In Vitro Is Greatly Enhanced by the Use of Recombinant Virus Lacking the US2 to US11 Region or Modified Vaccinia Virus Ankara Expressing Individual Viral Genes. J. Virol. 79: 2869-2879 [Abstract] [Full Text]  
  • Manley, T. J., Luy, L., Jones, T., Boeckh, M., Mutimer, H., Riddell, S. R. (2004). Immune evasion proteins of human cytomegalovirus do not prevent a diverse CD8+ cytotoxic T-cell response in natural infection. Blood 104: 1075-1082 [Abstract] [Full Text]  
  • Ferrari, G., Neal, W., Ottinger, J., Jones, A. M., Edwards, B. H., Goepfert, P., Betts, M. R., Koup, R. A., Buchbinder, S., McElrath, M. J., Tartaglia, J., Weinhold, K. J. (2004). Absence of Immunodominant Anti-Gag p17 (SL9) Responses among Gag CTL-Positive, HIV-Uninfected Vaccine Recipients Expressing the HLA-A*0201 Allele. J. Immunol. 173: 2126-2133 [Abstract] [Full Text]  
  • Wang, Z., La Rosa, C., Mekhoubad, S., Lacey, S. F., Villacres, M. C., Markel, S., Longmate, J., Ellenhorn, J. D. I., Siliciano, R. F., Buck, C., Britt, W. J., Diamond, D. J. (2004). Attenuated poxviruses generate clinically relevant frequencies of CMV-specific T cells. Blood 104: 847-856 [Abstract] [Full Text]  
  • Pelte, C., Cherepnev, G., Wang, Y., Schoenemann, C., Volk, H.-D., Kern, F. (2004). Random Screening of Proteins for HLA-A*0201-Binding Nine-Amino Acid Peptides Is Not Sufficient for Identifying CD8 T Cell Epitopes Recognized in the Context of HLA-A*0201. J. Immunol. 172: 6783-6789 [Abstract] [Full Text]  
  • Li Pira, G., Bottone, L., Ivaldi, F., Pelizzoli, R., Del Galdo, F., Lozzi, L., Bracci, L., Loregian, A., Palu, G., De Palma, R., Einsele, H., Manca, F. (2004). Identification of new Th peptides from the cytomegalovirus protein pp65 to design a peptide library for generation of CD4 T cell lines for cellular immunoreconstitution. Int Immunol 16: 635-642 [Abstract] [Full Text]  
  • Gibson, L., Piccinini, G., Lilleri, D., Revello, M. G., Wang, Z., Markel, S., Diamond, D. J., Luzuriaga, K. (2004). Human Cytomegalovirus Proteins pp65 and Immediate Early Protein 1 Are Common Targets for CD8+ T Cell Responses in Children with Congenital or Postnatal Human Cytomegalovirus Infection. J. Immunol. 172: 2256-2264 [Abstract] [Full Text]  
  • Brown, K., Gao, W., Alber, S., Trichel, A., Murphey-Corb, M., Watkins, S. C., Gambotto, A., Barratt-Boyes, S. M. (2003). Adenovirus-Transduced Dendritic Cells Injected into Skin or Lymph Node Prime Potent Simian Immunodeficiency Virus-Specific T Cell Immunity in Monkeys. J. Immunol. 171: 6875-6882 [Abstract] [Full Text]  
  • Gandhi, M. K., Wills, M. R., Okecha, G., Day, E. K., Hicks, R., Marcus, R. E., Sissons, J. G. P., Carmichael, A. J. (2003). Late diversification in the clonal composition of human cytomegalovirus-specific CD8+ T cells following allogeneic hemopoietic stem cell transplantation. Blood 102: 3427-3438 [Abstract] [Full Text]  
  • Karlsson, A. C., Deeks, S. G., Barbour, J. D., Heiken, B. D., Younger, S. R., Hoh, R., Lane, M., Sallberg, M., Ortiz, G. M., Demarest, J. F., Liegler, T., Grant, R. M., Martin, J. N., Nixon, D. F. (2003). Dual Pressure from Antiretroviral Therapy and Cell-Mediated Immune Response on the Human Immunodeficiency Virus Type 1 Protease Gene. J. Virol. 77: 6743-6752 [Abstract] [Full Text]  
  • Prod'homme, V., Retiere, C., Valtcheva, R., Bonneville, M., Hallet, M.-M. (2003). Cross-Reactivity of HLA-B*1801-Restricted T-Lymphocyte Clones with Target Cells Expressing Variants of the Human Cytomegalovirus 72kDa-IE1 Protein. J. Virol. 77: 7139-7142 [Abstract] [Full Text]  
  • Gallez-Hawkins, G., Villacres, M. C., Li, X., Sanborn, M. C., Lomeli, N. A., Zaia, J. A. (2003). Use of Transgenic HLA A*0201/Kb and HHD II Mice To Evaluate Frequency of Cytomegalovirus IE1-Derived Peptide Usage in Eliciting Human CD8 Cytokine Response. J. Virol. 77: 4457-4462 [Abstract] [Full Text]  
  • Gamadia, L. E., Remmerswaal, E. B. M., Weel, J. F., Bemelman, F., van Lier, R. A. W., Ten Berge, I. J. M. (2003). Primary immune responses to human CMV: a critical role for IFN-gamma -producing CD4+ T cells in protection against CMV disease. Blood 101: 2686-2692 [Abstract] [Full Text]  
  • Karrer, U., Sierro, S., Wagner, M., Oxenius, A., Hengel, H., Koszinowski, U. H., Phillips, R. E., Klenerman, P. (2003). Memory Inflation: Continuous Accumulation of Antiviral CD8+ T Cells Over Time. J. Immunol. 170: 2022-2029 [Abstract] [Full Text]  
  • Prod'homme, V., Retiere, C., Imbert-Marcille, B.-M., Bonneville, M., Hallet, M.-M. (2003). Modulation of HLA-A*0201-Restricted T Cell Responses by Natural Polymorphism in the IE1315-324 Epitope of Human Cytomegalovirus. J. Immunol. 170: 2030-2036 [Abstract] [Full Text]  
  • Addo, M. M., Yu, X. G., Rathod, A., Cohen, D., Eldridge, R. L., Strick, D., Johnston, M. N., Corcoran, C., Wurcel, A. G., Fitzpatrick, C. A., Feeney, M. E., Rodriguez, W. R., Basgoz, N., Draenert, R., Stone, D. R., Brander, C., Goulder, P. J. R., Rosenberg, E. S., Altfeld, M., Walker, B. D. (2003). Comprehensive Epitope Analysis of Human Immunodeficiency Virus Type 1 (HIV-1)-Specific T-Cell Responses Directed against the Entire Expressed HIV-1 Genome Demonstrate Broadly Directed Responses, but No Correlation to Viral Load. J. Virol. 77: 2081-2092 [Abstract] [Full Text]  
  • Lenfant, F., Pizzato, N., Liang, S., Davrinche, C., Le Bouteiller, P., Horuzsko, A. (2003). Induction of HLA-G-restricted human cytomegalovirus pp65 (UL83)-specific cytotoxic T lymphocytes in HLA-G transgenic mice. J. Gen. Virol. 84: 307-317 [Abstract] [Full Text]  
  • Khan, N., Shariff, N., Cobbold, M., Bruton, R., Ainsworth, J. A., Sinclair, A. J., Nayak, L., Moss, P. A. H. (2002). Cytomegalovirus Seropositivity Drives the CD8 T Cell Repertoire Toward Greater Clonality in Healthy Elderly Individuals. J. Immunol. 169: 1984-1992 [Abstract] [Full Text]  
  • Le Roy, E., Baron, M., Faigle, W., Clement, D., Lewinsohn, D. M., Streblow, D. N., Nelson, J. A., Amigorena, S., Davignon, J.-L. (2002). Infection of APC by Human Cytomegalovirus Controlled Through Recognition of Endogenous Nuclear Immediate Early Protein 1 by Specific CD4+ T Lymphocytes. J. Immunol. 169: 1293-1301 [Abstract] [Full Text]  
  • Wills, M R, Carmichael, A J, Sissons, J G P (2002). Vaccines against persistent DNA virus infections. Br Med Bull 62: 125-138 [Abstract] [Full Text]  
  • Amara, R. R., Villinger, F., Staprans, S. I., Altman, J. D., Montefiori, D. C., Kozyr, N. L., Xu, Y., Wyatt, L. S., Earl, P. L., Herndon, J. G., McClure, H. M., Moss, B., Robinson, H. L. (2002). Different Patterns of Immune Responses but Similar Control of a Simian-Human Immunodeficiency Virus 89.6P Mucosal Challenge by Modified Vaccinia Virus Ankara (MVA) and DNA/MVA Vaccines. J. Virol. 76: 7625-7631 [Abstract] [Full Text]  
  • Wills, M. R., Okecha, G., Weekes, M. P., Gandhi, M. K., Sissons, P. J. G., Carmichael, A. J. (2002). Identification of Naive or Antigen-Experienced Human CD8+ T Cells by Expression of Costimulation and Chemokine Receptors: Analysis of the Human Cytomegalovirus-Specific CD8+ T Cell Response. J. Immunol. 168: 5455-5464 [Abstract] [Full Text]  
  • Amara, R. R., Smith, J. M., Staprans, S. I., Montefiori, D. C., Villinger, F., Altman, J. D., O'Neil, S. P., Kozyr, N. L., Xu, Y., Wyatt, L. S., Earl, P. L., Herndon, J. G., McNicholl, J. M., McClure, H. M., Moss, B., Robinson, H. L. (2002). Critical Role for Env as well as Gag-Pol in Control of a Simian-Human Immunodeficiency Virus 89.6P Challenge by a DNA Prime/Recombinant Modified Vaccinia Virus Ankara Vaccine. J. Virol. 76: 6138-6146 [Abstract] [Full Text]  
  • Morello, C. S., Ye, M., Spector, D. H. (2002). Development of a Vaccine against Murine Cytomegalovirus (MCMV), Consisting of Plasmid DNA and Formalin-Inactivated MCMV, That Provides Long-Term, Complete Protection against Viral Replication. J. Virol. 76: 4822-4835 [Abstract] [Full Text]  
  • Moutaftsi, M., Mehl, A. M., Borysiewicz, L. K., Tabi, Z. (2002). Human cytomegalovirus inhibits maturation and impairs function of monocyte-derived dendritic cells. Blood 99: 2913-2921 [Abstract] [Full Text]  
  • Holtappels, R., Thomas, D., Podlech, J., Reddehase, M. J. (2002). Two Antigenic Peptides from Genes m123 and m164 of Murine Cytomegalovirus Quantitatively Dominate CD8 T-Cell Memory in the H-2d Haplotype. J. Virol. 76: 151-164 [Abstract] [Full Text]  
  • Peggs, K., Verfuerth, S., Pizzey, A., Ainsworth, J., Moss, P., Mackinnon, S. (2002). Characterization of human cytomegalovirus peptide-specific CD8+ T-cell repertoire diversity following in vitro restimulation by antigen-pulsed dendritic cells. Blood 99: 213-223 [Abstract] [Full Text]  
  • Gallot, G., Vivien, R., Ibisch, C., Lule, J., Davrinche, C., Gaschet, J., Vie, H. (2001). Purification of Ag-Specific T Lymphocytes After Direct Peripheral Blood Mononuclear Cell Stimulation Followed by CD25 Selection. I. Application to CD4+ or CD8+ Cytomegalovirus Phosphoprotein pp65 Epitope Determination. J. Immunol. 167: 4196-4206 [Abstract] [Full Text]  
  • Vaz-Santiago, J., Lule, J., Rohrlich, P., Jacquier, C., Gibert, N., Le Roy, E., Betbeder, D., Davignon, J.-L., Davrinche, C. (2001). Ex Vivo Stimulation and Expansion of both CD4+ and CD8+ T Cells from Peripheral Blood Mononuclear Cells of Human Cytomegalovirus-Seropositive Blood Donors by Using a Soluble Recombinant Chimeric Protein, IE1-pp65. J. Virol. 75: 7840-7847 [Abstract] [Full Text]  
  • Gratama, J. W., van Esser, J. W. J., Lamers, C. H. J., Tournay, C., Lowenberg, B., Bolhuis, R. L. H., Cornelissen, J. J. (2001). Tetramer-based quantification of cytomegalovirus (CMV)-specific CD8+ T lymphocytes in T-cell-depleted stem cell grafts and after transplantation may identify patients at risk for progressive CMV infection. Blood 98: 1358-1364 [Abstract] [Full Text]  
  • Bitmansour, A. D., Waldrop, S. L., Pitcher, C. J., Khatamzas, E., Kern, F., Maino, V. C., Picker, L. J. (2001). Clonotypic Structure of the Human CD4+ Memory T Cell Response to Cytomegalovirus. J. Immunol. 167: 1151-1163 [Abstract] [Full Text]  
  • Gamadia, L. E., Rentenaar, R. J., Baars, P. A., Remmerswaal, E. B. M., Surachno, S., Weel, J. F. L., Toebes, M., Schumacher, T. N. M., ten Berge, I. J. M., van Lier, R. A. W. (2001). Differentiation of cytomegalovirus-specific CD8+ T cells in healthy and immunosuppressed virus carriers. Blood 98: 754-761 [Abstract] [Full Text]  
  • Casazza, J. P., Betts, M. R., Picker, L. J., Koup, R. A. (2001). Decay Kinetics of Human Immunodeficiency Virus-Specific CD8+ T Cells in Peripheral Blood after Initiation of Highly Active Antiretroviral Therapy. J. Virol. 75: 6508-6516 [Abstract] [Full Text]  
  • Novak, E. J., Liu, A. W., Gebe, J. A., Falk, B. A., Nepom, G. T., Koelle, D. M., Kwok, W. W. (2001). Tetramer-Guided Epitope Mapping: Rapid Identification and Characterization of Immunodominant CD4+ T Cell Epitopes from Complex Antigens. J. Immunol. 166: 6665-6670 [Abstract] [Full Text]  
  • Tabi, Z., Moutaftsi, M., Borysiewicz, L. K. (2001). Human Cytomegalovirus pp65- and Immediate Early 1 Antigen-Specific HLA Class I-Restricted Cytotoxic T Cell Responses Induced by Cross-Presentation of Viral Antigens. J. Immunol. 166: 5695-5703 [Abstract] [Full Text]  
  • Koelle, D. M., Chen, H. B., Gavin, M. A., Wald, A., Kwok, W. W., Corey, L. (2001). CD8 CTL from Genital Herpes Simplex Lesions: Recognition of Viral Tegument and Immediate Early Proteins and Lysis of Infected Cutaneous Cells. J. Immunol. 166: 4049-4058 [Abstract] [Full Text]  
  • Peggs, K., Verfuerth, S., Mackinnon, S. (2001). Induction of cytomegalovirus (CMV)-specific T-cell responses using dendritic cells pulsed with CMV antigen: a novel culture system free of live CMV virions. Blood 97: 994-1000 [Abstract] [Full Text]  
  • Lewinsohn, D. M., Zhu, L., Madison, V. J., Dillon, D. C., Fling, S. P., Reed, S. G., Grabstein, K. H., Alderson, M. R. (2001). Classically Restricted Human CD8+ T Lymphocytes Derived from Mycobacterium tuberculosis-Infected Cells: Definition of Antigenic Specificity. J. Immunol. 166: 439-446 [Abstract] [Full Text]  
  • Holtappels, R., Thomas, D., Reddehase, M. J. (2000). Identification of a Kd-restricted antigenic peptide encoded by murine cytomegalovirus early gene M84. J. Gen. Virol. 81: 3037-3042 [Abstract] [Full Text]  
  • Betts, M. R., Casazza, J. P., Patterson, B. A., Waldrop, S., Trigona, W., Fu, T.-M., Kern, F., Picker, L. J., Koup, R. A. (2000). Putative Immunodominant Human Immunodeficiency Virus-Specific CD8+ T-Cell Responses Cannot Be Predicted by Major Histocompatibility Complex Class I Haplotype. J. Virol. 74: 9144-9151 [Abstract] [Full Text]  
  • Pepperl, S., Münster, J., Mach, M., Harris, J. R., Plachter, B. (2000). Dense Bodies of Human Cytomegalovirus Induce both Humoral and Cellular Immune Responses in the Absence of Viral Gene Expression. J. Virol. 74: 6132-6146 [Abstract] [Full Text]  
  • Retière, C., Prod'homme, V., Imbert-Marcille, B.-M., Bonneville, M., Vié, H., Hallet, M.-M. (2000). Generation of Cytomegalovirus-Specific Human T-Lymphocyte Clones by Using Autologous B-Lymphoblastoid Cells with Stable Expression of pp65 or IE1 Proteins: a Tool To Study the Fine Specificity of the Antiviral Response. J. Virol. 74: 3948-3952 [Abstract] [Full Text]  
  • Morello, C. S., Cranmer, L. D., Spector, D. H. (2000). Suppression of Murine Cytomegalovirus (MCMV) Replication with a DNA Vaccine Encoding MCMV M84 (a Homolog of Human Cytomegalovirus pp65). J. Virol. 74: 3696-3708 [Abstract] [Full Text]  
  • Zaia, J. A., Sissons, J.G. P., Riddell, S., Diamond, D. J., Wills, M.R., Carmichael, A.J., Weekes, M.P., Gandhi, M., La Rosa, C., Villacres, M., Lacey, S., Markel, S., Sun, J. (2000). Status of Cytomegalovirus Prevention and Treatment in 2000. ASH Education Book 2000: 339-355 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»