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Journal of Virology, October 1999, p. 8053-8063, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of the Complete DNA Sequences of Human Herpesvirus 6 Variants A and B

Yuji Isegawa,* Tetsu Mukai,dagger Kazushi Nakano, Miwa Kagawa, Jiguo Chen, Yasuko Mori, Tomimasa Sunagawa, Kazunobu Kawanishi, Junji Sashihara, Atsuko Hata, Ping Zou, Haruhiko Kosuge, and Koichi Yamanishi

Department of Microbiology, Osaka University Medical School C1, 2-2 Yamada-Oka Suita, Osaka 565-0871, Japan

Received 19 April 1999/Accepted 14 June 1999

Human herpesvirus 6 (HHV-6), which belongs to the betaherpesvirus subfamily and infects mainly T cells in vitro, causes acute and latent infections. Two variants of HHV-6 have been distinguished on the basis of differences in several properties. We have determined the complete DNA sequence of HHV-6 variant B (HHV-6B) strain HST, the causative agent of exanthem subitum, and compared the sequence with that of variant A strain U1102. A total of 115 potential open reading frames (ORFs) were identified within the 161,573-bp contiguous sequence of the entire HHV-6 genome, including some genes with remarkable differences in amino acid identity. All genes with <70% identity between the two variants were found to contain deleted regions when ORFs that could not be expressed were excluded from the comparison. Except in the case of U47, these differences were found in immediate-early/regulatory genes, DR2, DR7, U86/90, U89/90, and U95, which may represent characteristic differences of variants A and B. Also, we have successfully typed 14 different strains belonging to variant A or B by PCR using variant-specific primers; the results suggest that the remarkable differences observed were conserved evolutionarily as variant-specific divergence.

* Corresponding author. Mailing address: Department of Microbiology, Osaka University Medical School C1, 2-2 Yamada-Oka Suita, Osaka 565-0871, Japan. Phone: 81-6-6879-3323. Fax: 81-6-6879-3329. E-mail: isegawa{at}micro.med.osaka-u.ac.jp.

dagger Present address: Department of Virology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-Oka Suita, Osaka 565-0871, Japan.

Journal of Virology, October 1999, p. 8053-8063, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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