This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dominguez, G.
Right arrow Articles by Pellett, P. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dominguez, G.
Right arrow Articles by Pellett, P. E.

 Previous Article  |  Next Article 

Journal of Virology, October 1999, p. 8040-8052, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Human Herpesvirus 6B Genome Sequence: Coding Content and Comparison with Human Herpesvirus 6A

Geraldina Dominguez,1 Timothy R. Dambaugh,2 Felicia R. Stamey,1 Stephen Dewhurst,3 Naoki Inoue,1 and Philip E. Pellett1,*

Centers for Disease Control and Prevention, Atlanta, Georgia 303331; Qualicon, Inc., Wilmington, Delaware 198802; and University of Rochester Medical Center, Rochester, New York, 146423

Received 12 April 1999/Accepted 10 June 1999

Human herpesvirus 6 variants A and B (HHV-6A and HHV-6B) are closely related viruses that can be readily distinguished by comparison of restriction endonuclease profiles and nucleotide sequences. The viruses are similar with respect to genomic and genetic organization, and their genomes cross-hybridize extensively, but they differ in biological and epidemiologic features. Differences include infectivity of T-cell lines, patterns of reactivity with monoclonal antibodies, and disease associations. Here we report the complete genome sequence of HHV-6B strain Z29 [HHV-6B(Z29)], describe its genetic content, and present an analysis of the relationships between HHV-6A and HHV-6B. As sequenced, the HHV-6B(Z29) genome is 162,114 bp long and is composed of a 144,528-bp unique segment (U) bracketed by 8,793-bp direct repeats (DR). The genomic sequence allows prediction of a total of 119 unique open reading frames (ORFs), 9 of which are present only in HHV-6B. Splicing is predicted in 11 genes, resulting in the 119 ORFs composing 97 unique genes. The overall nucleotide sequence identity between HHV-6A and HHV-6B is 90%. The most divergent regions are DR and the right end of U, spanning ORFs U86 to U100. These regions have 85 and 72% nucleotide sequence identity, respectively. The amino acid sequences of 13 of the 17 ORFs at the right end of U differ by more than 10%, with the notable exception of U94, the adeno-associated virus type 2 rep homolog, which differs by only 2.4%. This region also includes putative cis-acting sequences that are likely to be involved in transcriptional regulation of the major immediate-early locus. The catalog of variant-specific genetic differences resulting from our comparison of the genome sequences adds support to previous data indicating that HHV-6A and HHV-6B are distinct herpesvirus species.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, 1600 Clifton Road, MS G18, Atlanta, GA 30333. Phone: (404) 639-2186. Fax: (404) 639-0049. E-mail: php1{at}cdc.gov.

Journal of Virology, October 1999, p. 8040-8052, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hall, C. B., Caserta, M. T., Schnabel, K., Shelley, L. M., Marino, A. S., Carnahan, J. A., Yoo, C., Lofthus, G. K., McDermott, M. P. (2008). Chromosomal Integration of Human Herpesvirus 6 Is the Major Mode of Congenital Human Herpesvirus 6 Infection. Pediatrics 122: 513-520 [Abstract] [Full Text]  
  • Flamand, L., Gravel, A., Boutolleau, D., Alvarez-Lafuente, R., Jacobson, S., Malnati, M. S., Kohn, D., Tang, Y.-W., Yoshikawa, T., Ablashi, D. (2008). Multicenter Comparison of PCR Assays for Detection of Human Herpesvirus 6 DNA in Serum. J. Clin. Microbiol. 46: 2700-2706 [Abstract] [Full Text]  
  • Isegawa, Y., Miyamoto, Y., Yasuda, Y., Semi, K., Tsujimura, K., Fukunaga, R., Ohshima, A., Horiguchi, Y., Yoneda, Y., Sugimoto, N. (2008). Characterization of the Human Herpesvirus 6 U69 Gene Product and Identification of Its Nuclear Localization Signal. J. Virol. 82: 710-718 [Abstract] [Full Text]  
  • Yeo, W. M., Isegawa, Y., Chow, V. T. K. (2008). The U95 Protein of Human Herpesvirus 6B Interacts with Human GRIM-19: Silencing of U95 Expression Reduces Viral Load and Abrogates Loss of Mitochondrial Membrane Potential. J. Virol. 82: 1011-1020 [Abstract] [Full Text]  
  • Kofod-Olsen, E., Ross-Hansen, K., Mikkelsen, J. G., Hollsberg, P. (2008). Human herpesvirus 6B U19 protein is a PML-regulated transcriptional activator that localizes to nuclear foci in a PML-independent manner. J. Gen. Virol. 89: 106-116 [Abstract] [Full Text]  
  • Tavakoli, N. P., Nattanmai, S., Hull, R., Fusco, H., Dzigua, L., Wang, H., Dupuis, M. (2007). Detection and Typing of Human Herpesvirus 6 by Molecular Methods in Specimens from Patients Diagnosed with Encephalitis or Meningitis. J. Clin. Microbiol. 45: 3972-3978 [Abstract] [Full Text]  
  • Crawford, J. R., Kadom, N., Santi, M. R., Mariani, B., Lavenstein, B. L. (2007). Human Herpesvirus 6 Rhombencephalitis in Immunocompetent Children. J Child Neurol 22: 1260-1268 [Abstract]  
  • Pedersen, S. M., Oster, B., Bundgaard, B., Hollsberg, P. (2006). Induction of Cell-Cell Fusion from Without by Human Herpesvirus 6B. J. Virol. 80: 9916-9920 [Abstract] [Full Text]  
  • Ehlers, B., Dural, G., Marschall, M., Schregel, V., Goltz, M., Hentschke, J. (2006). Endotheliotropic elephant herpesvirus, the first betaherpesvirus with a thymidine kinase gene.. J. Gen. Virol. 87: 2781-2789 [Abstract] [Full Text]  
  • Tomoiu, A., Gravel, A., Tanguay, R. M., Flamand, L. (2006). Functional Interaction between Human Herpesvirus 6 Immediate-Early 2 Protein and Ubiquitin-Conjugating Enzyme 9 in the Absence of Sumoylation.. J. Virol. 80: 10218-10228 [Abstract] [Full Text]  
  • Dewin, D. R., Catusse, J., Gompels, U. A. (2006). Identification and Characterization of U83A Viral Chemokine, a Broad and Potent {beta}-Chemokine Agonist for Human CCRs with Unique Selectivity and Inhibition by Spliced Isoform. J. Immunol. 176: 544-556 [Abstract] [Full Text]  
  • Zhen, Z., Bradel-Tretheway, B., Sumagin, S., Bidlack, J. M., Dewhurst, S. (2005). The Human Herpesvirus 6 G Protein-Coupled Receptor Homolog U51 Positively Regulates Virus Replication and Enhances Cell-Cell Fusion In Vitro. J. Virol. 79: 11914-11924 [Abstract] [Full Text]  
  • Lacoste, V., Verschoor, E. J., Nerrienet, E., Gessain, A. (2005). A novel homologue of Human herpesvirus 6 in chimpanzees. J. Gen. Virol. 86: 2135-2140 [Abstract] [Full Text]  
  • Donati, D., Martinelli, E., Cassiani-Ingoni, R., Ahlqvist, J., Hou, J., Major, E. O., Jacobson, S. (2005). Variant-Specific Tropism of Human Herpesvirus 6 in Human Astrocytes. J. Virol. 79: 9439-9448 [Abstract] [Full Text]  
  • Tadagaki, K., Nakano, K., Yamanishi, K. (2005). Human Herpesvirus 7 Open Reading Frames U12 and U51 Encode Functional {beta}-Chemokine Receptors. J. Virol. 79: 7068-7076 [Abstract] [Full Text]  
  • De Bolle, L., Naesens, L., De Clercq, E. (2005). Update on Human Herpesvirus 6 Biology, Clinical Features, and Therapy. Clin. Microbiol. Rev. 18: 217-245 [Abstract] [Full Text]  
  • van Cleef, K. W. R., Scaf, W. M. A., Maes, K., Kaptein, S. J. F., Beuken, E., Beisser, P. S., Stassen, F. R. M., Grauls, G. E. L. M., Bruggeman, C. A., Vink, C. (2004). The rat cytomegalovirus homologue of parvoviral rep genes, r127, encodes a nuclear protein with single- and double-stranded DNA-binding activity that is dispensable for virus replication. J. Gen. Virol. 85: 2001-2013 [Abstract] [Full Text]  
  • Dietrich, J., Blumberg, B. M., Roshal, M., Baker, J. V., Hurley, S. D., Mayer-Proschel, M., Mock, D. J. (2004). Infection with an Endemic Human Herpesvirus Disrupts Critical Glial Precursor Cell Properties. J. Neurosci. 24: 4875-4883 [Abstract] [Full Text]  
  • Mori, Y., Akkapaiboon, P., Yonemoto, S., Koike, M., Takemoto, M., Sadaoka, T., Sasamoto, Y., Konishi, S., Uchiyama, Y., Yamanishi, K. (2004). Discovery of a Second Form of Tripartite Complex Containing gH-gL of Human Herpesvirus 6 and Observations on CD46. J. Virol. 78: 4609-4616 [Abstract] [Full Text]  
  • Borenstein, R., Singer, O., Moseri, A., Frenkel, N. (2004). Use of Amplicon-6 Vectors Derived from Human Herpesvirus 6 for Efficient Expression of Membrane-Associated and -Secreted Proteins in T Cells. J. Virol. 78: 4730-4743 [Abstract] [Full Text]  
  • Gruijthuijsen, Y. K., Beuken, E. V. H., Smit, M. J., Leurs, R., Bruggeman, C. A., Vink, C. (2004). Mutational analysis of the R33-encoded G protein-coupled receptor of rat cytomegalovirus: identification of amino acid residues critical for cellular localization and ligand-independent signalling. J. Gen. Virol. 85: 897-909 [Abstract] [Full Text]  
  • Kondo, K., Nozaki, H., Shimada, K., Yamanishi, K. (2003). Detection of a Gene Cluster That Is Dispensable for Human Herpesvirus 6 Replication and Latency. J. Virol. 77: 10719-10724 [Abstract] [Full Text]  
  • Tam, A., Zhu, J., Hai, R., Haghjoo, E., Tong, T., Zhan, X., Lu, S., Liu, F. (2003). Murine Cytomegalovirus with a Transposon Insertional Mutation at Open Reading Frame M35 Is Defective in Growth In Vivo. J. Virol. 77: 7746-7755 [Abstract] [Full Text]  
  • Nakano, K., Tadagaki, K., Isegawa, Y., Aye, M. M., Zou, P., Yamanishi, K. (2003). Human Herpesvirus 7 Open Reading Frame U12 Encodes a Functional {beta}-Chemokine Receptor. J. Virol. 77: 8108-8115 [Abstract] [Full Text]  
  • Perelygina, L., Zhu, L., Zurkuhlen, H., Mills, R., Borodovsky, M., Hilliard, J. K. (2003). Complete Sequence and Comparative Analysis of the Genome of Herpes B Virus (Cercopithecine Herpesvirus 1) from a Rhesus Monkey. J. Virol. 77: 6167-6177 [Abstract] [Full Text]  
  • Mori, Y., Akkapaiboon, P., Yang, X., Yamanishi, K. (2003). The Human Herpesvirus 6 U100 Gene Product Is the Third Component of the gH-gL Glycoprotein Complex on the Viral Envelope. J. Virol. 77: 2452-2458 [Abstract] [Full Text]  
  • Kondo, K., Sashihara, J., Shimada, K., Takemoto, M., Amo, K., Miyagawa, H., Yamanishi, K. (2003). Recognition of a Novel Stage of Betaherpesvirus Latency in Human Herpesvirus 6. J. Virol. 77: 2258-2264 [Abstract] [Full Text]  
  • Davison, A. J., Dolan, A., Akter, P., Addison, C., Dargan, D. J., Alcendor, D. J., McGeoch, D. J., Hayward, G. S. (2003). The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome. J. Gen. Virol. 84: 17-28 [Abstract] [Full Text]  
  • Dockrell, D.H. (2003). Human herpesvirus 6: molecular biology and clinical features. J Med Microbiol 52: 5-18 [Abstract] [Full Text]  
  • Stanton, R., Fox, J. D., Caswell, R., Sherratt, E., Wilkinson, G. W. G. (2002). Analysis of the human herpesvirus-6 immediate-early 1 protein. J. Gen. Virol. 83: 2811-2820 [Abstract] [Full Text]  
  • De Bolle, L., Michel, D., Mertens, T., Manichanh, C., Agut, H., De Clercq, E., Naesens, L. (2002). Role of the Human Herpesvirus 6 U69-Encoded Kinase in the Phosphorylation of Ganciclovir. Mol. Pharmacol. 62: 714-721 [Abstract] [Full Text]  
  • Janelle, M.-E., Gravel, A., Gosselin, J., Tremblay, M. J., Flamand, L. (2002). Activation of Monocyte Cyclooxygenase-2 Gene Expression by Human Herpesvirus 6. ROLE FOR CYCLIC AMP-RESPONSIVE ELEMENT-BINDING PROTEIN AND ACTIVATOR PROTEIN-1. J. Biol. Chem. 277: 30665-30674 [Abstract] [Full Text]  
  • Collot, S., Petit, B., Bordessoule, D., Alain, S., Touati, M., Denis, F., Ranger-Rogez, S. (2002). Real-Time PCR for Quantification of Human Herpesvirus 6 DNA from Lymph Nodes and Saliva. J. Clin. Microbiol. 40: 2445-2451 [Abstract] [Full Text]  
  • Oster, B., Hollsberg, P. (2002). Viral Gene Expression Patterns in Human Herpesvirus 6B-Infected T Cells. J. Virol. 76: 7578-7586 [Abstract] [Full Text]  
  • Mori, Y., Seya, T., Huang, H. L., Akkapaiboon, P., Dhepakson, P., Yamanishi, K. (2002). Human Herpesvirus 6 Variant A but Not Variant B Induces Fusion from Without in a Variety of Human Cells through a Human Herpesvirus 6 Entry Receptor, CD46. J. Virol. 76: 6750-6761 [Abstract] [Full Text]  
  • Gravel, A., Gosselin, J., Flamand, L. (2002). Human Herpesvirus 6 Immediate-Early 1 Protein Is a Sumoylated Nuclear Phosphoprotein Colocalizing with Promyelocytic Leukemia Protein-associated Nuclear Bodies. J. Biol. Chem. 277: 19679-19687 [Abstract] [Full Text]  
  • Dhepakson, P., Mori, Y., Jiang, Y. B., Huang, H. L., Akkapaiboon, P., Okuno, T., Yamanishi, K. (2002). Human herpesvirus-6 rep/U94 gene product has single-stranded DNA-binding activity. J. Gen. Virol. 83: 847-854 [Abstract] [Full Text]  
  • Kondo, K., Shimada, K., Sashihara, J., Tanaka-Taya, K., Yamanishi, K. (2002). Identification of Human Herpesvirus 6 Latency-Associated Transcripts. J. Virol. 76: 4145-4151 [Abstract] [Full Text]  
  • Gruijthuijsen, Y. K., Casarosa, P., Kaptein, S. J. F., Broers, J. L. V., Leurs, R., Bruggeman, C. A., Smit, M. J., Vink, C. (2002). The Rat Cytomegalovirus R33-Encoded G Protein-Coupled Receptor Signals in a Constitutive Fashion. J. Virol. 76: 1328-1338 [Abstract] [Full Text]  
  • Franti, M., Gessain, A., Darlu, P., Gautheret-Dejean, A., Kosuge, H., Mauclere, P., Aubin, J.-T., Gurtsevitch, V., Yamanishi, K., Agut, H. (2001). Genetic polymorphism of human herpesvirus-7 among human populations. J. Gen. Virol. 82: 3045-3050 [Abstract] [Full Text]  
  • Manichanh, C., Olivier-Aubron, C., Lagarde, J.-P., Aubin, J.-T., Bossi, P., Gautheret-Dejean, A., Huraux, J.-M., Agut, H. (2001). Selection of the same mutation in the U69 protein kinase gene of human herpesvirus-6 after prolonged exposure to ganciclovir in vitro and in vivo. J. Gen. Virol. 82: 2767-2776 [Abstract] [Full Text]  
  • Takemoto, M., Shimamoto, T., Isegawa, Y., Yamanishi, K. (2001). The R3 Region, One of Three Major Repetitive Regions of Human Herpesvirus 6, Is a Strong Enhancer of Immediate-Early Gene U95. J. Virol. 75: 10149-10160 [Abstract] [Full Text]  
  • Van den Bosch, G., Locatelli, G., Geerts, L., Faga, G., Ieven, M., Goossens, H., Bottiger, D., Oberg, B., Lusso, P., Berneman, Z. N. (2001). Development of Reverse Transcriptase PCR Assays for Detection of Active Human Herpesvirus 6 Infection. J. Clin. Microbiol. 39: 2308-2310 [Abstract] [Full Text]  
  • Xu, Y., Linde, A., Dahl, H., Winberg, G. (2001). Definition of a Divergent Epitope That Allows Differential Detection of Early Protein p41 from Human Herpesvirus 6 Variants A and B. J. Clin. Microbiol. 39: 1449-1455 [Abstract] [Full Text]  
  • Abenes, G., Lee, M., Haghjoo, E., Tong, T., Zhan, X., Liu, F. (2001). Murine Cytomegalovirus Open Reading Frame M27 Plays an Important Role in Growth and Virulence in Mice. J. Virol. 75: 1697-1707 [Abstract] [Full Text]  
  • LaCroix, S., Stewart, J. A., Thouless, M. E., Black, J. B. (2000). An Immunoblot Assay for Detection of Immunoglobulin M Antibody to Human Herpesvirus 6. CVI 7: 823-827 [Abstract] [Full Text]  
  • Johnson, G., Nelson, S., Petric, M., Tellier, R. (2000). Comprehensive PCR-Based Assay for Detection and Species Identification of Human Herpesviruses. J. Clin. Microbiol. 38: 3274-3279 [Abstract] [Full Text]  
  • Vink, C., Beuken, E., Bruggeman, C. A. (2000). Complete DNA Sequence of the Rat Cytomegalovirus Genome. J. Virol. 74: 7656-7665 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»