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Journal of Virology, October 1999, p. 8035-8039, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Simian Immunodeficiency Virus Envelope Glycoprotein Contains Two Epitopes Presented by the Mamu-A*01 Class I Molecule

Michelle Furchner,1 Ann L. Erickson,1 Todd Allen,2 David I. Watkins,2 Alessandro Sette,3 Philip R. Johnson,1 and Christopher M. Walker1,*

Children's Research Institute, Children's Hospital, Inc., and Department of Pediatrics, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio1; Department of Pathology, Wisconsin Regional Primate Center, University of Wisconsin, Madison, Wisconsin2; and Epimmune Corporation, San Diego, California3

Received 5 April 1999/Accepted 22 June 1999

Cytotoxic T lymphocyte (CTL) responses against the simian immunodeficiency virus (SIV) envelope and Gag proteins were monitored in a Mamu-A*01-positive rhesus macaque infected with SIVsmE660. Peripheral blood mononuclear cells (PBMC) cultured with synthetic peptides spanning the entire gp160 and Gag coding region recognized a total of three epitopes. One located in Gag was identified as the previously described Mamu-A*01-restricted p11cCright-arrowM epitope (CTPYDINQM). The other two epitopes, designated p15m and p54m, were located in the gp160 envelope protein. Both were nine amino acids in length and were predicted to bind Mamu-A*01 because they contained proline and leucine residues at positions 3 and 9, respectively. Indeed, expression of this class I major histocompatibility complex molecule was required for target cell recognition by envelope-specific CD8+ T cells directed against both epitopes. These Mamu-A*01-restricted epitopes in the SIV envelope will be useful for monitoring immune responses in vaccinated or infected animals.

* Corresponding author. Mailing address: Children's Hospital, W503, 700 Children's Dr., Columbus, OH 43205. Phone: (614) 722-2692. Fax: (614) 722-3273. E-mail: WalkerC{at}pediatrics.ohio-state.edu.

Journal of Virology, October 1999, p. 8035-8039, Vol. 73, No. 10
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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