Previous Article | Next Article ![]()
Journal of Virology, October 1999, p. 7952-7964, Vol. 73, No. 10
Department of Macromolecular Structure,
Centro Nacional de Biotecnología, Campus Universidad
Autónoma, Cantoblanco, 28049 Madrid, Spain
Received 11 March 1999/Accepted 23 June 1999
During the life cycle of the transmissible gastroenteritis
coronavirus (TGEV), two types of virus-related particles are detected in infected swine testis cells: large annular viruses and small dense
viruses. We have studied the relationships between these two types of
particles. Immunoelectron microscopy showed that they are closely
related, since both large and small particles reacted equally with
polyclonal and monoclonal antibodies specific for TGEV proteins.
Monensin, a drug that selectively affects the Golgi complex, caused an
accumulation of large annular viral particles in perinuclear elements
of the endoplasmic reticulum-Golgi intermediate compartment. A partial
reversion of the monensin blockade was obtained in both the absence and
presence of cycloheximide, a drug that prevented the formation of new
viral particles. After removal of monensin, the Golgi complex recovered
its perinuclear location, and a decrease in the number of perinuclear
large viral particles was observed. The release of small dense viral
particles into secretory vesicles and the extracellular medium was also observed, as was a partial recovery of infectivity in culture supernatants. Small viral particles started to be seen between the
third and the fourth Golgi cisternae of normally infected cells. All of
these data strongly indicate that the large annular particles are the
immature precursors of the small dense viruses, which are the
infectious TGEV virions. The immature viral particles need to reach a
particular location at the trans side of the Golgi stack to
complete their morphological maturation.
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Structural Maturation of the Transmissible
Gastroenteritis Coronavirus
*
Corresponding author. Mailing address: Department of
Macromolecular Structure, Centro Nacional de Biotecnología
(CSIC), Campus Universidad Autónoma, Cantoblanco, 28049 Madrid,
Spain. Phone: 34-91-5854550. Fax: 34-91-5854506. E-mail:
crisco{at}cnb.uam.es.
This article has been cited by other articles:
| J. Bacteriol. | Mol. Cell. Biol. | Microbiol. Mol. Biol. Rev. |
|---|
| Clin. Vaccine Immunol. | ALL ASM JOURNALS |
|---|